Fourteen discrete fragments ranging from 25 bp to 650 bp in 50 bp increments
Double intensity reference bands at 200 bp and 400 bp
The Norgen MiniSizer 50 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains fourteen discrete fragments ranging from 25 bp to 650 bp in 50 bp increments with double intensity reference bands at 200 bp and 400 bp. The MiniSizer is ideal for PCR product size confirmation.
Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
MiniSizer 50 bp DNA Ladder (Cat# 11200) – 100 loads
Ladder Properties: • Fourteen discrete bands, ranging from 50 bp to 650 bp • Higher intensity bands at 200 bp and 400 bp for easy reference
Fragment
Size (bp)
Mass (ng)
1
650
61
2
600
46
3
550
36
4
500
30
5
450
35
6
400
85
7
350
27
8
300
25
9
250
16
10
200
54
11
150
33
12
100
21
13
50
17
14
25
14
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Other Products
Plasma/Serum Circulating DNA Purification Kits (Slurry Format)
Product Info
Document
Product Info
Overview
Isolate all sizes of circulating DNA from plasma and serum samples
Isolate Viral and Bacterial DNA
Versatile plasma and serum input volumes
Concentrate circulating DNA into small elution volumes
Different elution strategies depending on the downstream application
Isolate inhibitor-free circulating DNA for any application including PCR, qPCR, methylation-sensitive PCR
Purification is based on Norgen’s proprietary Silicon Carbide resin matrix
Norgen’s Plasma/Serum Circulating DNA Purification Kits (Slurry Format) provide efficient methods for the purification of fragmented free-circulating DNA from human plasma or serum. The kit is able to isolate all sizes of circulating DNA. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating DNA from large sample volumes. DNA can be isolated from either fresh or frozen samples using this kit. The kit will also isolate viral and bacterial DNA from plasma/serum. Typical yields of purified free-circulating DNA will vary depending on the input sample (1-100ng/mL circulating DNA in human plasma), with more concentrated samples tending to yield more free-circulating nucleic acids. The purified, inhibitor-free plasma/serum free-circulating DNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis.
Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format)
Norgen’s Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 50 μL to 400 μL. Preparation time for a single sample is less than 30 minutes.
Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format)
Norgen’s Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 400 μL to 2 mL. Preparation time for a single sample is less than 45 minutes.
Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format)
Norgen’s Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 2 mL to 10 mL. Preparation time for a single sample is less than 45 minutes.
Plasma/Serum Circulating and Exosomal RNA Purification Kits (Slurry Format)
Product Info
Document
Product Info
Overview
Isolate all sizes of circulating and exosomal RNA, including microRNA
Versatile plasma and serum input volumes
Concentrate circulating and exosomal RNA into small elution volumes
Isolate inhibitor-free circulating and exosomal RNA
Bind and elute all RNA irrespective of size or GC content, without bias
Available in Spin Column or 96-well plate formats
Process 96-well plates using vacuum or centrifugation
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
Compatible with Streck Cell-Free RNA BCT® Tubes
Purification is based on Norgen’s proprietary resin matrix
These kits are able to isolate all sizes of circulating and exosomal RNA, including microRNA, without the use of phenol or chloroform. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating and exosomal RNA from large sample volumes. RNA can be isolated from either fresh or frozen samples using this kit. This kit is suitable for the isolation of RNA from serum or plasma prepared from blood collected only on either EDTA or citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications including RT-PCR. The purified plasma/serum free-circulating and exosomal RNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.
Free-circulating plasma and serum RNA can serve as both tumor- and fetal-specific markers for cancer detection and prenatal diagnosis. As well, free-circulating RNAs have the potential to provide biomarkers for other disease states. Free-circulating RNA in plasma or serum are usually present as short fragments of less than 1000nt, and free-circulating miRNA (21nt) can also be found in plasma and serum.
Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry)
Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating RNA and exosomal RNA from plasma and serum samples ranging from 0.25 mL to 5 mL.
Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Mini Slurry)
Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating and exosomal RNA from plasma and serum samples ranging from 0.25 mL to 2 mL.
Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification 96-Well Kit (Slurry Format) provides a high throughput method for isolating circulating and exosomal RNA from plasma and serum samples using either a vacuum manifold or centrifugation.
Plasma/Serum Circulating and Exosomal RNA Purification Maxi Kit (Maxi Slurry)
Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Maxi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating RNA and exosomal RNA from various amounts of plasma/serum ranging from 2 mL to 5 mL.
Cat.29500 Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
TGM12 Table top capillary vessel Haematocrit centrifuge
Product Info
Document
Product Info
TGM12 Table top capillary vessel Haematocrit centrifuge
Application:
Measure the ratio of blood corpuscle and separate microblood and micro solution.
Model: TGM12 Table top high speed capillary vessel centrifuge
Features:
Brushless DC motor, free maintenance, no powder pollution, micro computer control, digital display indicates the time、speed and RCF. Electric lid lock、 over speed and imbalance protection. The centrifuge body is made of high quality steel, Safe and reliable.
