Nineteen discrete fragments ranging from 100 bp to 10000 bp
Higher intensity reference bands at 500 bp, 1000 bp and 5000 bp
The Norgen HighRangerPlus 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our HighRangerPlus contains nineteen discrete fragments ranging from 100 bp to 10000 bp with higher intensity reference bands at 500 bp, 1000 bp and 5000 bp. This Ladder is ideal for size determination of digested DNA.
Contents: 1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
HighRanger Plus 100 bp DNA Ladder (Cat# 12000) – 100 loads
Ladder Properties:
Nineteen discrete fragments ranging from 100 bp to 10000 bp
Higher intensity bands at 500 bp, 1000 bp and 5000 bp for easy reference
Fragment
Size (bp)
Mass (ng)
1
10000
42
2
8000
33
3
6000
25
4
5000
42
5
4000
35
6
3000
27
7
2500
22
8
2000
18
9
1500
13
10
1000
40
11
900
28
12
800
25
13
700
34
14
600
19
15
500
44
16
400
13
17
300
18
18
200
13
19
100
10
Recommended Use: Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage: This kit is stable for 2 years after the date of shipment.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
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