Overview
- Eight discrete bands, ranging from 100 to 1,000 bases
- Higher intensity band at 500 bases for easy reference
- No need for staining or destaining as loading dye contains ethidium bromide
- Convenient lyophilized format provides better product stability
The Norgen 100 b RNA Ladder is a set of RNA transcripts derived from recombinant DNA templates. This ladder is suitable for precise sizing of small RNA molecules using native or denaturing agarose gels, and can be easily visualized under UV.
Contents
- 2 vials of lyophilized RNA ladder (25 loads each)
- 1x loading buffer: 33.3% formamide, 11.7% formaldehyde, 0.012% bromophenol blue, 0.05mM EDTA, 0.012% ethidium bromide, and 0.7x MOPS solution
- 2x loading buffer
Details
Supporting Data
Figure 1 / 1
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Specifications:
- Eight discrete bands, ranging from 100 to 1,000 bases
- Higher intensity band at 500 bases for easy reference
Contents:
- 2 vials of lyophilized RNA ladder (25 loads each)
- 1x loading buffer: 33.3% formamide, 11.7% formaldehyde, 0.012% bromophenol blue, 0.05mM EDTA, 0.012% ethidium bromide, and 0.7x MOPS solution
- 2x loading buffer
Instructions:
To reconstitute the lyophilized RNA ladder, add 250 µL of 1x loading buffer to each 25 loads vial and vortex gently.
Heat at 80°C for 10 minutes. Incubate on ice for 1 min. Load 10 µL on a 1.5-2% gel. For optimal results, use Norgen 2x loading buffer with each RNA sample. There is no need for staining and destaining denaturing gels since Norgen’s loading buffer contains ethidium bromide.
Storage:
Store at -20°C.
