RNA/DNA/Protein Purification Plus Kits

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Detail

Overview

  • Sequentially purify total RNA (and miRNA), DNA and proteins from a single sample
  • No sample splitting or need to use phenol or precipitation methods
  • Purify RNA/DNA/Protein from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

RNA/DNA/Protein Purification Plus Kit

This kit provides a rapid method for the high throughput isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, or yeast. The kit employs two columns: 1) for gDNA purification and 2) for RNA purification utilizing Norgen’s resin columns (superior for the binding of all RNA sizes including miRNA). The proteins are also purified on the second column after RNA elution. The proteins are eluted in buffer and are ready for downstream application without any further clean up required. The proteins can be quantified directly, used in western blots, ELISA or mass spectrometry. This kit provides a rapid spin-column method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants.

Workflow
Workflow

RNA/DNA/Protein Purification Plus Micro Kit

This kit provides a rapid spin-column method for the isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, microdissected samples including LCM, stem cells, sorted cells, and CTC. The total RNA, genomic DNA and proteins are all column purified in less than 30 minutes. The RNA and DNA can be eluted in as little as 20 µL while the protein can be eluted in as little as 50 µL. This kit provides the same performance as if the samples were isolated from dedicated kits.

RNA/DNA/Protein Purification 96-Well Plus Kit

The kit employs two plates: 1) for DNA purification and 2) for RNA purification utilizing Norgen’s resin (superior for the binding of all RNA sizes including miRNA). Please see the protocol schematic below.

Details

Supporting Data

Figure 1 / 4

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RNA/DNA/Protein Purification Plus Kit Figure 1
RNA/DNA/Protein Purification Plus Kit Figure 1
RNA/DNA/Protein Purification Plus Kit Figure 2
RNA/DNA/Protein Purification Plus Kit Figure 3
RNA/DNA/Protein Purification Plus Kit Figure 4

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Kit Specifications
Maximum Column Binding Capacity50 μg for RNA
20 μg for DNA
200 μg for protein
Maximum Column Loading Volume650 μL
Size of RNA PurifiedAll sizes, including small RNA
(< 200 nt)
Size of DNA Purified≥ 30 kb
Maximum Amount of Starting Material:
Animal Cells
Animal Tissues
Blood
Bacteria
Yeast
Fungi
Plant Tissues
5 x 106 cells
25 mg (for selected tissues)
100 μL
1 x 109 cells
1 x 108 cells
50 mg
50 mg
Time to Complete 10 Purifications30 minutes
Average Yield:HeLa Cells (1 x 106 cells)HeLa Cells (1 x 106 cells)HeLa Cells (1 x 106 cells)
15 μg RNA8 μg DNA150 μg protein

 
Storage Conditions and Product Stability
The Protein Loading Dye should be stored at -20°C after the addition of DL-Dithiothreitol (DTT). All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

ComponentCat. 47700 (50 preps)Cat. 51600 (50 preps)Cat. 51700 (96 preps)
Buffer SKP40 mL40 mL
Lysis Buffer Q40 mL
Wash Solution A2 x 38 mL
1 x 18 mL
2 x 38 mL
1 x 18 mL
2 x 38 mL
1 x 18 mL
Elution Solution A6 mL6 mL20 mL
Elution Buffer F15 mL15 mL2 x 15 mL
Wash Solution C30 mL30 mL60 mL
Binding Buffer A8 mL8 mL8 mL
Elution Buffer C8 mL8 mL30 mL
Protein Neutralizer4 mL4 mL4 mL
Protein Loading Dye2 mL2 mL3 x 2 mL
gDNA Purification Columns50
gDNA Purification Micro Columns50
gDNA Purification 96-Well Plate1
RNA/Protein Purification Columns50
RNA/Protein Purification Micro Columns50
RNA/Protein Purification 96-Well Plate1
Collection Tubes150150
Collection Plate5
Elution Tubes (1.7 mL)150150
Elution Plate3
Lysis Preparation Plate2
Adhesive Tape4
Product Insert111

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