Overview
- Rapid and efficient removal of abundant proteins from serum and plasma samples
- Process 10 samples in 30 minutes
- Allows for visualization of low abundance proteins
- Convenient and affordable spin column protocol
- Generic protocol allows for depletion from human and other animal samples
- Purification is based on spin column chromatography that uses Norgen’s resin separation matrix
This kit provides a fast and simple procedure for the effective depletion of major serum proteins including albumin, α-antitrypsin, transferrin and haptoglobin from serum and plasma samples. Such abundant proteins usually obscure less abundant proteins in gel electrophresis making them difficult to visualize and recover. The kit reduces sample complexity to allow for convenient investigation of less abundant proteins. In other applications such as mass spectroscopy, fractionating abundant proteins improves the resolution of less abundant proteins. The kit is ‘generic’ in that it is not antibody-based and can thus be used to deplete abundant proteins from any starting material – human or various other animals – making it more versatile, flexible and affordable than other kits. Resulting proteins are ready for a range of applications including 2D gel electrophoresis, SDS-PAGE, Mass Spectrometry, Protein microarrays, DIGE (Difference Gel Electrophoresis) and more.
Details
Supporting Data
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| Kit Specifications | |
| Maximum Column Capacity | 500 μg |
| Minimum Column Capacity | 200 μg |
| Minimum Elution Volume | 30 μL |
| Time to Process 10 Samples | 30 minutes |
Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 17300 (25 preps) |
|---|---|
| Wash Solution G | 125 mL |
| Elution Buffer C | 8 mL |
| Protein Neutralizer | 4 mL |
| Spin Columns | 25 |
| Collection Tubes | 25 |
| Elution Tubes (1.7 mL) | 25 |
| Product Insert | 1 |
