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Human Papillomavirus (HPV) (High Risk) TaqMan PCR Detection Kits

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Detail

Overview

  • Detection kits for the HPV (High Risk)
  • Available in TaqMan format for analysis

More than 70 types of human papillomavirus (HPV) have been identified, and are generally classified as high-risk or low-risk depending on their relationship or lack of relationship with cancer and high-grade cervical intraepithelial neoplasia (CIN 2-3). HPV viruses are predominantly sexually transmitted and high-risk HPV types are a major risk factor for development of cervical cancer. Low-risk HPV types 6 and 11 have been associated with the presence of genital warts. There are many other low-risk HPV types that are not associated with genital warts or cervical cancer. Until now, HPV cannot be cultured in vitro, and immunological tests are inadequate to determine the presence of HPV cervical infection. On the other hand, biopsies can be analyzed by nucleic acid hybridization to directly detect the presence of HPV DNA. HPV 16 and HPV 18 have been considered as high-risk cancer associated HPV types.

HPV (High Risk) TaqMan PCR Kit, 100 reactions

  • Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
  • Specific Primer and Probe mix for the pathogen/virus/viroid of interest
  • Primer and Probe mix
  • Positive and negative control to confirm the integrity of the kit reagents

HPV (High Risk) TaqMan PCR Probe/Primer Set and Controls, 100 reactions

  • Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
  • Nuclease-free water
  • Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

Figure 1 / 5

Previous

Figure 1. Example of TaqMan PCR Positive result
Figure 1. Example of TaqMan PCR Positive result
Figure 2. Example of TaqMan PCR Positive (Cy5) result
Figure 3. Example of TaqMan PCR Positive (FAM) result
Figure 4. Example of TaqMan PCR Negative result
Figure 5. Example of TaqMan PCR inhibition result

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

ComponentCat. TM32250 (100 preps)Cat. TM32210 (100 preps)
MDx TaqMan 2X PCR Master Mix2 x 700 μL
HPV (High Risk) Primer & Probe Mix280 μL280 μL
HPV (High Risk) Positive Control150 μL150 μL
Nuclease-Free Water (Negative Control)1.25 mL1.25 mL
Product Insert11

Other Products

endo-BCN-PEG8-PFP ester

endo-BCN-PEG8-PFP ester is a PEG linker featuring a BCN group and a PFP ester. BCN is a click chemistry handle used to react with azides on target molecules while the PFP is a good leaving group which is readily displaced by amines to form amides.

16×10ml Angle Rotor 

D-Glucose Assay Kit (Megaplex Red)

K-MRGLUC

1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser)

Content:1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser)
Shipping Temperature:Ambient
Storage Temperature:Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability:> 2 years under recommended storage conditions
Analyte:D-Glucose
Assay Format:Spectrophotometer, Microplate, Auto-analyser
Detection Method:Absorbance, Fluorescence
Wavelength (nm):(Fluorescence) 530-560 (ex), ~ 590 (em)
(Absorbance) 570
Signal Response:Increase
Linear Range:(Fluorescence) 0.009-0.18 μg of D-glucose per assay (0.5-10 μM in-assay)
(Absorbance) 0.9-9.0 μg of D-glucose per assay (5-50 μM in-assay)
Limit of Detection:(Fluorescence) 0.09 ug/mL
(Absorbance)
 0.25 ug/mL
Reproducibility (%):(Fluorescence) ~ 6%
(Absorbance) ~ 7%
Reaction Time (min):(Fluorescence) 30 min
(Absorbance) 
30 min
Application examples:Food and beverage samples such as wine, beer, fruit juices, soft drinks, milk, jam, dietetic foods, bakery products, candies, fruit and vegetables. Other samples such as tobacco, cosmetics, pharmaceuticals (e.g. infusions), feed, paper (and cardboard) and other materials (e.g. biological cultures, samples, etc.).

This product has been discontinued.

The D-Glucose Assay Kit (Megaplex Red) provides a simple robust method for the measurement of D-glucose. This kit utilises a highly sensitive Megaplex Red probe which when coupled to horseradish peroxidase (HRP) and glucose oxidase (GOX) enzymatic reactions, allows for the measurement of D-glucose in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation between 530-560 nm and emission at ~ 590 nm* or colourimetrically at 570 nm.

*The excitation and emission maxima of Megaplex Red are 570 nm and 585 nm respectively.

See more related mono/disaccharide assay kit products. 

Fluorometric/UV method for the determination of D-glucose content in a variety of samples.

Scheme-K-MRGLUC

Advantages

  • Simple format
  • Very competitive price (cost per test)
  • Ability to run in fluorescence or absorbance mode
  • User friendly – Detailed protocol provided for the creation of calibration curve and the calculation of concentration in fluorescence mode. Single point standard for quicker and simpler analysis in absorbance mode.
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Suitable for manual, microplate and auto-analyser formats
  • All reagents stable for > 2 years