Overview
- Ensure optimal results for sensitive applications like NGS
- Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
- Isolates all sizes of exosomal and extracellular vesicle RNA, including microRNA.
- Bind and elute all RNA irrespective of size or GC content, without bias.
- No phenol extractions.
- No Proteinase K treatment.
- No carrier RNA.
- Concentrate isolated RNA into a flexible elution volume ranging from 50 μL to 100 μL.
- Purify high-quality RNA in 15-20 minutes.
- Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing.
- Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary separation matrix.
The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Details
Supporting Data
Figure 1 / 5
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| Sample Type | Exosomes purified using Norgen’s Purification Kits |
| Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
| Elution Volume | 50-100 μL |
| Time to Complete 10 Purifications | 35-40 minutes |
| Average Yields | Variable depending on specimen |
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
