The 16S V1-V3 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V1-V3 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The 16S V1-V3 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V1-V3 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
Storage Conditions and Product Stability Norgen’s 16S V1-V3 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
Step
Component
Cat. 70200 (24 preps)
Cat. 70210, 70220, 70230, 70240 (96 preps)
Amplicon PCR (PCR 1)
MGX Master Mix
330 µL
1,320 µL
16S V1-V3 Primer Mix
70 µL
280 µL
Index PCR (PCR 2)
Indexing Master Mix
660 µL
2 x 1,320 µL
N7xx Index Primer
50 µL
50 µL
S5xx Index Primer
70 µL
70 µL
PCR Clean-Up
Resuspension Buffer
2 x 1,250 µL
2 x 5,000 µL
Nuclease-free water
1,250 µL
1 x 6,000 µL
Other Products
65L/ 90L/ 110L High-pressure Steam Autoclave
Product Info
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Product Info
Safety Features
1. User-Friendly Design: The chamber lid and tabletop are covered with heat-insulating resin materials, providing both protection against burns and an aesthetically pleasing appearance. The upward-opening flip-top door saves space, while the rounded corners prevent injury. The specially designed steam discharge and collection system minimizes steam burn risks and enhances operational convenience.
2. Interlock Mechanism and Safety Protection: The safety interlock system is designed to be more sensitive and secure. The chamber lid cannot be opened if there is pressure or steam inside the chamber. The lid can only be opened when the pressure gauge reads “0” MPa. The sterilizer will not start heating if the chamber lid is not properly locked. These features enhance the overall safety of the machine.
3. Over-temperature and Heating Protection: The system continuously monitors the temperature inside the sterilization chamber. If the temperature becomes too high or heating is abnormal, power will be automatically cut off to prevent overheating.
4. Automatic Fault Detection System: The machine performs an automatic self-check when powered on. If a fault occurs during operation, an audible alarm will sound, and a fault code will be displayed.
5. Lid Closure Check System: The system automatically detects whether the chamber lid is securely locked. If the lid is not properly locked, the sterilizer will not start.
6. Leakage, Over-current, and Short-Circuit Protection: If there is leakage in the heating elements or other components, or if over-current or short-circuit conditions occur, the system will automatically cut off power to prevent user injury.
7. Water Shortage and Independent Dry-Burning Protection: Equipped with a low water level sensor and independent dry-burning protection device, the system can monitor the water level in the chamber in real-time to prevent dry burning and avoid false readings. If the low water level sensor fails, the overheat and dry-burning protection system will automatically activate, cut off power to the heating ring, and sound an alarm to ensure safety.
8. Dual Pressure Protection: The system includes both a mechanical safety valve and an electronic over-pressure protection system. If pressure becomes abnormal, the system will relieve pressure and cut off power while sounding an alarm.
9. Back end Safety Testing: The system can be periodically tested for safety and protective measures.
10. Over-pressure Testing: Regular testing ensures that the instrument’s over-pressure protection system can automatically cut off power or that the safety valve can open to relieve pressure.
11. Performance Testing: The instrument’s safety performance has been tested by national authorized inspection agencies, and a test report is available.
Technical Parameters
Model
HB65A Standard TypeHB65B Auto Water Replenishment TypeHB65C Auto Drying Type
HB90AStandard TypeHB90BAuto Water Replenishment TypeHB90CAuto Drying Type
HB110AStandard TypeHB110BAuto Water Replenishment TypeHB110CAuto Drying Type
Capacity
65L
90L
110L
Dimensions (L × W × H) mm
664×660×994.5
664×660×994.5
664×660×1200
Sterilization Chamber Dimensions (Diameter × Height) mm
Timer Pre-set Range: Set operating time (year, month, day, hour, minute), check current time (year, month, day, hour, minute), total sterilization cycles, fault occurrence time, and code.
Pressure Gauge Display Range
0～0.6Mpa
Pressure
Chamber Design Pressure: 0.35 MPa; Each product comes with a quality certificate and a certificate issued by the special equipment manufacturing supervision and inspection department.
Pressure Safety Protection
Dual independent pipeline measurement and control, mechanical and electronic dual pressure protection
Fully automatic internal exhaust (see instructions for exhaust temperature settings), built-in large-capacity steam condensate collection bottle and water tank
Door lock interlocking device; Cover closing check; Dry burn protection; Water level sensor monitoring; Electronic over pressure protection; Mechanical safety valve; Over temperature and heating protection; Over current and short circuit protection; Leakage protection; Cooling lock; Burn injury safety protection; Automatic fault detection system
Printer, cooling fan, item temperature sensor, USB output
Stainless Steel Baskets
(φ360×230)1pcs(φ360×200)1pcs
(φ360×200)1pcs(φ360×400)1pcs
(φ360×200)2pcs(φ360×400)1pcs
Note: The upper limits for cooling lock door temperature and exhaust temperature are related to the boiling point.
Other Optional Accessories
1. Dot Matrix Printer: Capable of printing temperature, time, F0 value, and pressure data. USB data export and curve plotting.
2. Stainless Steel Basket with Base.
3. Pressure Sensor
4. Stainless Steel Bucket
5. Item Thermometer
6. 3Q Validation Adapter
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Features and functions:
1. Large-diameter sterilization chamber：The 40 cm large-diameter sterilization chamber provides an exceptionally spacious environment to meet a wide range of sterilization needs.
2. Sterilization temperature：105-138℃.
3. Exhaust control ：After sterilization is complete, the exhaust valve can automatically open to release the exhaust at a pre-set temperature.
4. Verification interface: Provides temperature and pressure inspection ports for easy calibration.
5. Saturated steam monitoring：The system automatically monitors the expulsion of cold air to ensure a pure steam sterilization environment, guaranteeing optimal sterilization effectiveness.
6. Pressure performance：The chamber is designed to withstand a pressure of up to 0.35 MPa, and each unit is tested with a water pressure of up to 0.44 MPa. Advanced pressure protection ensures the safety load of the equipment.
7. Intelligent control system:The microcomputer chip-based intelligent control system monitors the temperature and pressure inside the sterilization chamber in real-time. The chamber lid can only be opened under safe temperature and pressure conditions. The system also provides fully automatic control of the sterilization process and allows for convenient maintenance, inspection, calibration, recording, and future upgrades.
8. Six preset programs:Includes six fixed programs for liquid sterilization and holding, solid sterilization and drying, agar melting and holding, and waste sterilization.
9. Memory storage system:Users can set their own parameters, create multiple programs, and access them at any time. The system allows for operation permissions to be set at three levels—user, administrator, and engineer—to facilitate effective use and management of the sterilizer.
10. Door-opening temperature: Each program can be set with a specific cooling lock door-opening temperature based on the thermal inertia of the sterilized items. This prevents users from opening the door when the items (especially liquid sterilized items) are still in an unsafe condition, thus avoiding burns.
11. Drying function: Thorough drying that meets CE standards, with a cooling lock mechanism.
12. One-touch inquiry function: Allows checking of current time, total number of sterilization cycles, fault occurrence time, and codes.
13. Scheduled start function: Allows you to set a start time so that the sterilizer automatically starts at the scheduled time.
This product allows rapid and reliable isolation of high-quality genomic DNA from various soil, stool, and other environmental samples. Up to 500mg soil, 100mg stool, or 0.5g environmental samples can be processed in 60 minute. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 250-500mg soil sample
Applications
PCR, southern blot and enzyme digestion, etc.
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Soil
Sample amount
250-500mg
Elution volume
≥50μl
Time per run
≤70 minutes
Principle
This product is based on the purification method of high binding magnetic particles. Soil sample is homogenized and then treated in a specially formulated buffer containing detergent to lyse bacteria, yeast, and fungal samples. Humic acid, proteins, polysaccharides, and other contaminants are removed using our propietary Absorber Solution. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing buffer to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
High concentration – maximum extraction of total DNA from soil samples
High purity – purified DNA can be directly used in various downstream applications
Kit Contents
Contents
D635601
D635602
D635603
Purification Times
48 Preps
96 Preps
5 x 96 Preps
MagPure Particles
2.5 ml
5 ml
22 ml
2ml Bead Tubes
48
96
400
Buffer SOL
60 ml
100 ml
500 ml
Buffer SDS
6 ml
10 ml
50 ml
Reagent DX
1 ml
1 ml
5 ml
Buffer PS
10 ml
20 ml
90 ml
Absorber Solution
10 ml
20 ml
90 ml
Buffer GDP
70 ml
150 ml
2 x 350 ml
Buffer GW1*
22 ml
44 ml
220 ml
Elution Buffer
20 ml
20 ml
60 ml
Storage and Stability
MagPure Particles and Absorber Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
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This product allows rapid and reliable isolation of high-quality genomic DNA from various soil, stool, and other environmental samples. Up to 500mg soil, 100mg stool, or 0.5g environmental samples can be processed in 60 minute. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing.
The NGS DNA Library Prep Kit (Ion Torrent platform) was developed for construction of high quality DNA libraries for next generation sequencing using Ion Torrent platform. The kit uses short double strand DNA fragments (blunt ends and/or sticky ends) as input DNA for NGS library construction, and is compatible with DNA fragments generated from both enzymatic methods (BioDynami DNA Fragmentation Enzyme Mix and DNA Fragmentation & A-tailing Enzyme Mix etc) and mechanical methods (sonication, nebulization etc.). Our unique technology increases library conversion efficiency and eliminates insert concatemer ligation. Library multiplexing up to 12 samples is possible.
NGS DNA Library Prep Kit Workflow
Kit features
Simple procedure: Reactions for all three steps are in one tube
Fast protocol
Total protocol time is around 1 hour
Hands-on time is only ~5 minutes
Guaranteed high library conversion efficiency as compared to other kits
Input DNA amount: from 50 ng to 1 ug
No insert concatemer ligation
Document
The NGS DNA Library Prep Kit (Ion Torrent platform) was developed for construction of high quality DNA libraries for next generation sequencing using Ion Torrent platform. The kit uses short double strand DNA fragments (blunt ends and/or sticky ends) as input DNA for NGS library construction, and is compatible with DNA fragments generated from both enzymatic methods (BioDynami DNA Fragmentation Enzyme Mix and DNA Fragmentation & A-tailing Enzyme Mix etc) and mechanical methods (sonication, nebulization etc.). Our unique technology increases library conversion efficiency and eliminates insert concatemer ligation. Library multiplexing up to 12 samples is possible.
