Rapid spin column purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positive
Bead tubes (provided) allow for effective mechanical homogenization
Purified DNA is of high quality and integrity and compatible with any sensitive downstream applications such as PCR, qPCR, RFLP and more
These kits provide fast and reliable procedures for the purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positive. Genomic DNA is efficiently extracted from the cells by a combination of heat treatment, detergents and Bead Tubes (provided). An optional lyticase treatment allows for improved DNA yields with certain fungal and yeast species. Recovered genomic DNA is of excellent yield and purity for any downstream application including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), Amplified Fragment Length Polymorphism (AFLP), sequencing, SNP analysis and more.
Fungi/Yeast Genomic DNA Isolation Kit (Spin Column)
This kit provides rapid spin column purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positive. Preparation time for a single sample is less then 30 minutes, and each kit contains sufficient materials for 50 preparations.
Fungi/Yeast Genomic DNA Isolation 96-Well Kit (HT)
Norgen’s Fungi/Yeast Genomic DNA Isolation 96-Well Kit provides a fast, reliable and simple procedure for high throughput isolation of DNA from viable yeast cells, fungal spores or mycelium and Gram-positive bacteria. The purification could be performed on either a vacuum manifold or using centrifugation. Complete 96 purifications in 40 minutes.
* Yield will vary depending on the type of sample processed
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component
Cat. 27300 (50 preps)
Cat. 27350 (192 preps)
Lysis Buffer L
30 mL
2 x 60 mL
Resuspension Solution A
20 mL
60 mL
Solution BX
28 mL
2 x 28 mL
Wash Solution A
18 mL
2 x 38 mL
Elution Buffer B
15 mL
30 mL
Bead Tubes
50
200
Spin Columns
50
–
Collection Tubes
50
–
96-Well Plate
–
2
96-Well Collection Plate
–
2
Adhesive Tape
–
4
Elution Tubes (1.7 mL)
50
–
96-Well Elution Plate
–
2
Product Insert
1
1
Other Products
N-(Propargyl-PEG2)-DBCO-PEG3-NHS ester
Product Info
Document
Product Info
N-(Propargyl-PEG2)-DBCO-PEG3-NHS ester is a PEG linker with a terminal NHS ester to perform facile reactions with amine groups of molecules as well as a propargyl to react with azides to form a triazole. The DBCO can participate in copper-free Click Chemistry reactions.
Document
N-(Propargyl-PEG2)-DBCO-PEG3-NHS ester is a PEG linker with a terminal NHS ester to perform facile reactions with amine groups of molecules as well as a propargyl to react with azides to form a triazole. The DBCO can participate in copper-free Click Chemistry reactions.
Yingtai pilot-type freeze dryer completely simulates the technical requirements of the industrial freeze dryer,with a compact and reasonable design,a large redundancy range,and conforms to GMP and FDA standards.The ice condensation capacity is 4~18kg,and the WRL controller and liquid temperature control shelf are adopted,so that the process of pre-freezing and freeze-drying can be precisely controlled on the main machine,and the process results are quite comparable with the production machine.In addition to supporting the eutectic point test system,pressure rise and pressure comparison method to determine the freeze-drying end point and freeze-drying curve recording software,hallan pilot-type freeze dryer can also be equipped with engineering WRL control software to directly Graphical computer control the freeze-drying process.It can be configured with H202 sterilization and high-pressure steam sterilization orintegrated glove box system, which can be used for clean room operation through the wall.The cold trap and freeze-drying chamber are all designed with 316L stainless steel to ensure the cleanliness of the chamber and good ultimate vacuum and vacuum leakage rate to the greatest extent.
Refrigeration Series
● Dual compressors,Secop special compressors,air cooling (water cooling system is optional)
Silicone oil conducts heat,with uniform temperature and accuracy up to 0.1°C.Silicone oil has a wide temperature range,up to-100°C~+600°C.
● Shelf refrigeration system and cold trap refrigeration system are completely independent,without any switching,refrigeration temperature is lower and more stable.
Vacuum series
● Vacuum pump:German Pfeiffer anti-corrosion,anti-oil return function vacuum pump.(Vacuum pump can be selected according to user needs)
● Pumping rate:9m³/h~62m³/h,multiple specifications are optional,the ultimate vacuum can reach 2×10-3mbar,and the oil mist filter is standard.
● The vacuum pipelines are all high-vacuum stainless steel vacuum tubes, which can effectively prevent vacuum leakage and corrosion.
● The double anti-oil return device completely eliminates the damage and
equipment and samples.
● Automatic backfill air device,the system has a built-in High efficiency particulate air(HEPA)filter to ensure the complete cleanliness of backfill air.
● The vacuum stabilization system can stabilize the vacuum degree of the freeze-drying process through the micro-pass vacuum control valve to ensure that the samples will not be affected by excessive fluctuations in the vacuum degree.
● Condensed liquid collection and discharge system,automatically completes the collection and discharge of condensed liquid after freeze-drying.
Control SystemCharacteristic
● Control:Automatic/Manual
● Can store at least 100 independent freeze-drying programs,each program contains 32 program segments (Can be
customized according to user needs),each program segment corresponds to a processing stage (pre-freezing,main
drying,post-drying).and Include the following segment values:Cycles,shelf temperature,vacuum and safety pressure
● All the equipment can be recorded in real time and the corresponding freeze-drying curves and data tables can be generated after the end.The records of all data and curves comply with the corresponding regulations and standards.
● It has hierarchical management authority,hierarchical management allocation authority and usage process tracking authority
● Remote System
O Fully support 2G/4G/GSM/Wi-Fivarious connection modes
o Users can remotely view all data on the device and operate it
o Technicians can remotely perform maintenance and fault detection on equipment,and can remotely guide and train users
o Can remotely upgrade and change the system of the equipment
(Optional)Sufficient amount and time for the program to run itself
User function customization
Can be customized and modified according to user needs
Sterilization method
HzOz/steam sterilization
Note:”/”optional.Pilot models can be customized according to user needs
Document
Yingtai pilot-type freeze dryer completely simulates the technical requirements of the industrial freeze dryer,with a compact and reasonable design,a large redundancy range,and conforms to GMP and FDA standards.The ice condensation capacity is 4~18kg,and the WRL controller and liquid temperature control shelf are adopted,so that the process of pre-freezing and freeze-drying can be precisely controlled on the main machine,and the process results are quite comparable with the production machine.In addition to supporting the eutectic point test system,pressure rise and pressure comparison method to determine the freeze-drying end point and freeze-drying curve recording software,hallan pilot-type freeze dryer can also be equipped with engineering WRL control software to directly Graphical computer control the freeze-drying process.It can be configured with H202 sterilization and high-pressure steam sterilization orintegrated glove box system, which can be used for clean room operation through the wall.The cold trap and freeze-drying chamber are all designed with 316L stainless steel to ensure the cleanliness of the chamber and good ultimate vacuum and vacuum leakage rate to the greatest extent.
Microcystin are a class of hepatotoxins produced by blue-green algae such as Microcystis aeruginosa. Microcystin-LR is the most common of the over 50 different congeners. Cyanobacteria can produce microcystin in large quantities during an algae bloom which then pose a major threat.
This kit can be used for Congener-Independent quantitative test of Microcystin in liquid samples such as drinking, ambient and waste water and algal cultures.
Additional Quality Control, Calibration Verification, and Spiking Solution materials can be obtained separately in optional Supplemental Pack for Method 546 (catalog # EL2024-Q).
Microcystin-LR
Informational sign postings about HABs at recreational waters: < 6 μg/L
Recreational public health advisory: 6 μg/L
Elevated recreational public health advisory (e.g. no contact): 20 μg/L
EPA 10-day drinking water health advisories:
Do Not Drink – 0.3 μg/L for bottle fed infants and preschool children, pregnant and nursing woman, elderly immunocompromised and liver conditions.
Do Not Drink – 1.6 μg/L for school-age children to adults.
Do not Use – 20 μg/L
Document
Format: 96-well microtiter plate (12 test strips of 8 wells) Standards: 0 | 0.15 | 0.4 | 1 | 2 | 5 ppb Incubation Time: 75 Minutes Compatible for use with US EPA Method 546
