High yield and high quality genomic DNA with no RNA or protein contamination
DNA ready for any application including PCR, qPCR, genotyping and more
Norgen’s Cells and Tissue DNA Isolation Kits are designed for the rapid preparation of genomic DNA from cultured cells as well as various tissue samples and urine. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with PCR and Southern Blot analysis.
Cells and Tissue DNA Isolation Kit (Spin Column)
Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The protocol can be completed in approximately 30 minutes for cells and within 90 minutes for tissues. Each kit contains sufficient materials for 50 preparations.
Cells and Tissue DNA Isolation Micro Kit (Micro)
Optimized for small inputs of cells and tissues, such as Laser-Captured Microdissection (LCM). Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. Preparation time for a single sample is approximately 60 minutes, and each kit contains sufficient materials for 50 preparations.
Cells and Tissue DNA Isolation Kit (Magnetic Bead System)
Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. Preparation for 10 purifications is approximately 40 minutes of hands-on time.
Cells and Tissue DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. The Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) also can be integrated with a robotic automation system.
20 mg of animal tissue Up to 150 μL of viral suspension 3 x 106 cells
Column Binding Capacity
> 50 μg
Average Yield
8 μg (from 1 x 106 HeLa Cells) 10 μg (from 10 mg kidney)
Elution Volume
50 – 200 μL
Analyte Purified
Genomic DNA, mitochondrial DNA, viral DNA
Format
Spin Column
Time to Complete 10 Purifications
30 min (cells) and 90 min (tissue)
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
Component
Cat. 53100 (50 preps)
Cat. 57300 (50 preps)
Cat. 59100 (50 preps)
Cat. 62500 (192 preps)
Lysis Buffer B
20 mL
20 mL
20 mL
1 x 40 mL 1 x 20 mL
Solution WN
18 mL
18 mL
18 mL
55 mL
Wash Solution A
18 mL
18 mL
–
–
Elution Buffer B
30 mL
8 mL
15 mL
1 x 30 mL 1 x 15 mL
Proteinase K
1.2 mL
1.2 mL
1.2 mL
–
Proteinase K in Sotrage Buffer
–
–
–
4 mL
Magnetic Bead Suspension
–
–
2 x 1.1 mL
8.5 mL
96-Well Plate
–
–
–
2
Spin Columns
50
–
–
–
Micro Spin Columns
–
50
–
–
Collection Tubes
50
50
–
–
Elution Tubes (1.7 mL)
50
50
50
–
96-Well Elution Plate
–
–
–
2
Adhesive Tape
–
–
–
2
Product Insert
1
1
1
1
Other Products
Sulfo DBCO-PEG3-NHS ester
Product Info
Document
Product Info
Sulfo DBCO-PEG3-NHS ester is a water soluble, click chemistry reagent which can undergo copper free click chemistry Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Sulfo DBCO-PEG3-NHS ester is a water soluble, click chemistry reagent which can undergo copper free click chemistry Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
1. User-Friendly Design: The chamber lid and tabletop are covered with heat-insulating resin materials, providing both protection against burns and an aesthetically pleasing appearance. The upward-opening flip-top door saves space, while the rounded corners prevent injury. The specially designed steam discharge and collection system minimizes steam burn risks and enhances operational convenience.
2. Interlock Mechanism and Safety Protection: The safety interlock system is designed to be more sensitive and secure. The chamber lid cannot be opened if there is pressure or steam inside the chamber. The lid can only be opened when the pressure gauge reads “0” MPa. The sterilizer will not start heating if the chamber lid is not properly locked. These features enhance the overall safety of the machine.
3. Over-temperature and Heating Protection: The system continuously monitors the temperature inside the sterilization chamber. If the temperature becomes too high or heating is abnormal, power will be automatically cut off to prevent overheating.
4. Automatic Fault Detection System: The machine performs an automatic self-check when powered on. If a fault occurs during operation, an audible alarm will sound, and a fault code will be displayed.
5. Lid Closure Check System: The system automatically detects whether the chamber lid is securely locked. If the lid is not properly locked, the sterilizer will not start.
6. Leakage, Over-current, and Short-Circuit Protection: If there is leakage in the heating elements or other components, or if over-current or short-circuit conditions occur, the system will automatically cut off power to prevent user injury.
7. Water Shortage and Independent Dry-Burning Protection: Equipped with a low water level sensor and independent dry-burning protection device, the system can monitor the water level in the chamber in real-time to prevent dry burning and avoid false readings. If the low water level sensor fails, the overheat and dry-burning protection system will automatically activate, cut off power to the heating ring, and sound an alarm to ensure safety.
8. Dual Pressure Protection: The system includes both a mechanical safety valve and an electronic over-pressure protection system. If pressure becomes abnormal, the system will relieve pressure and cut off power while sounding an alarm.
9. Back end Safety Testing: The system can be periodically tested for safety and protective measures.
10. Over-pressure Testing: Regular testing ensures that the instrument’s over-pressure protection system can automatically cut off power or that the safety valve can open to relieve pressure.
11. Performance Testing: The instrument’s safety performance has been tested by national authorized inspection agencies, and a test report is available.
Technical Parameters
Model
HB65A Standard TypeHB65B Auto Water Replenishment TypeHB65C Auto Drying Type
HB90AStandard TypeHB90BAuto Water Replenishment TypeHB90CAuto Drying Type
HB110AStandard TypeHB110BAuto Water Replenishment TypeHB110CAuto Drying Type
Capacity
65L
90L
110L
Dimensions (L × W × H) mm
664×660×994.5
664×660×994.5
664×660×1200
Sterilization Chamber Dimensions (Diameter × Height) mm
Timer Pre-set Range: Set operating time (year, month, day, hour, minute), check current time (year, month, day, hour, minute), total sterilization cycles, fault occurrence time, and code.
Pressure Gauge Display Range
0～0.6Mpa
Pressure
Chamber Design Pressure: 0.35 MPa; Each product comes with a quality certificate and a certificate issued by the special equipment manufacturing supervision and inspection department.
Pressure Safety Protection
Dual independent pipeline measurement and control, mechanical and electronic dual pressure protection
Fully automatic internal exhaust (see instructions for exhaust temperature settings), built-in large-capacity steam condensate collection bottle and water tank
Door lock interlocking device; Cover closing check; Dry burn protection; Water level sensor monitoring; Electronic over pressure protection; Mechanical safety valve; Over temperature and heating protection; Over current and short circuit protection; Leakage protection; Cooling lock; Burn injury safety protection; Automatic fault detection system
Printer, cooling fan, item temperature sensor, USB output
Stainless Steel Baskets
(φ360×230)1pcs(φ360×200)1pcs
(φ360×200)1pcs(φ360×400)1pcs
(φ360×200)2pcs(φ360×400)1pcs
Note: The upper limits for cooling lock door temperature and exhaust temperature are related to the boiling point.
Other Optional Accessories
1. Dot Matrix Printer: Capable of printing temperature, time, F0 value, and pressure data. USB data export and curve plotting.
2. Stainless Steel Basket with Base.
3. Pressure Sensor
4. Stainless Steel Bucket
5. Item Thermometer
6. 3Q Validation Adapter
Document
Features and functions:
1. Large-diameter sterilization chamber：The 40 cm large-diameter sterilization chamber provides an exceptionally spacious environment to meet a wide range of sterilization needs.
2. Sterilization temperature：105-138℃.
3. Exhaust control ：After sterilization is complete, the exhaust valve can automatically open to release the exhaust at a pre-set temperature.
4. Verification interface: Provides temperature and pressure inspection ports for easy calibration.
5. Saturated steam monitoring：The system automatically monitors the expulsion of cold air to ensure a pure steam sterilization environment, guaranteeing optimal sterilization effectiveness.
6. Pressure performance：The chamber is designed to withstand a pressure of up to 0.35 MPa, and each unit is tested with a water pressure of up to 0.44 MPa. Advanced pressure protection ensures the safety load of the equipment.
7. Intelligent control system:The microcomputer chip-based intelligent control system monitors the temperature and pressure inside the sterilization chamber in real-time. The chamber lid can only be opened under safe temperature and pressure conditions. The system also provides fully automatic control of the sterilization process and allows for convenient maintenance, inspection, calibration, recording, and future upgrades.
8. Six preset programs:Includes six fixed programs for liquid sterilization and holding, solid sterilization and drying, agar melting and holding, and waste sterilization.
9. Memory storage system:Users can set their own parameters, create multiple programs, and access them at any time. The system allows for operation permissions to be set at three levels—user, administrator, and engineer—to facilitate effective use and management of the sterilizer.
10. Door-opening temperature: Each program can be set with a specific cooling lock door-opening temperature based on the thermal inertia of the sterilized items. This prevents users from opening the door when the items (especially liquid sterilized items) are still in an unsafe condition, thus avoiding burns.
11. Drying function: Thorough drying that meets CE standards, with a cooling lock mechanism.
12. One-touch inquiry function: Allows checking of current time, total number of sterilization cycles, fault occurrence time, and codes.
13. Scheduled start function: Allows you to set a start time so that the sterilizer automatically starts at the scheduled time.
