Urine DNA Isolation Kit Dx (Slurry Format)

Overview

• Detection kits for Candida albicans
• CE-IVD marked version available for in vitro diagnostic use
• Available in TaqMan format for analysis

Candida albicans is an opportunistic yeast and is the most common fungal pathogen found in the human body. C. albicans can be detected in the gastrointestinal tract, mouth, and vagina of approximately half of healthy adults. It is typically a commensal organism and makes up part of the natural human microflora; however it can overgrow and become pathogenic as a result of various conditions. For example, individuals who have taken recent courses of antibiotics, have a weakened immune system or have diabetes have an increased risk of developing a Candida albicans infection. When an overgrowth occurs, this can lead to common infections such as urinary tract infections, genital yeast infections, oral thrush, and mucocutaneous candidiasis. In the more severe cases, when Candida albicans enters the bloodstream or organs, it can lead to loss of sight, blood and bone infections, endocarditis, meningitis or inflammation of the intraabdominal lining.

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

Component	Cat. TM34050 (100 preps)	Cat. TM34010 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
Candida albicans Primer & Probe Mix	280 μL	280 μL
Candida albicans Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Overview

• Sequentially purify total RNA and total proteins from a single sample
• Kit includes a gDNA elimination column  
• No sample splitting required
• No phenol step required for efficient isolation
• Ideal for small or difficult to obtain samples
• Purify RNA and proteins from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants
• Rapid and efficient spin column procedure
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

This kit provides a rapid, single column method for the isolation and purification of total RNA (including miRNA) and proteins sequentially from a single sample of cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants. The total RNA and proteins are both column purified in under 25 minutes using a single column.

Purified RNA is of a high quality and yield, and is suitable for NGS, RT-qPCR and microarrays.  

This kit eliminates DNA efficiently using a gDNA removal column.

Proteins are eluted in buffer and are ready for downstream applications such as Western Blots, Mass Spec and ELISA.  The proteins will not require precipitation, resuspending of pellets, or any further cleaning.

This kit is ideal for researchers who are interested in studying the transcriptome and proteome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Norgen’s RNA/Protein Purification Plus Kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA and proteins are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications.

Protocol

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Supporting Data

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Kit Specifications
Maximum Column Binding Capacity	50 μg for RNA
Maximum Column Binding Capacity	200 μg for Protein
Maximum Column Loading Volume	650 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Time to Complete 10 Purifications	30 minutes
Average Yields*: HEK 293 Cells (1 x 106 cells) HEK 293 Cells (1 x 106 cells) Liver (15 mg) Liver (15 mg)	10-15 μg RNA 70-100 μg protein 30-35 μg RNA 100-150 μg protein

* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.

Storage Conditions and Product Stability
The Protein Loading Dye should be stored at -20°C after the addition of DL-Dithiothreitol (DTT). All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 48200 (50 preps)
Buffer SKP	40 mL
Wash Solution A	38 mL
Elution Solution A	6 mL
Wash Solution C	30 mL
Binding Buffer A	8 mL
Elution Buffer C	4 mL
Protein Neutralizer	4 mL
Protein Loading Dye	2 mL
gDNA Removal Columns	50
RNA/Protein Purification Columns	50
Collection Tubes	150
Elution Tubes (1.7 mL)	100
Product Insert	1