Everything you need to run a trial PACE® allele-specific PCR Genotyping Reaction on your existing lab equipment. Each PACE Trial Kit includes Test DNA samples, PACE Genotyping Assays, PACE Master Mix and a comprehensive PACE Genotyping Trial Kit Manual.
WHO IS THIS TRIAL KIT FOR?
Anyone who wants to try PACE genotyping reagents in their lab for the first time with a set of validated DNA samples, SNP assays and PACE Master Mix.
TRIAL KIT OVERVIEW
Step 1. Dispense each of the three trial DNA samples (DNA 1, 2 and 3) plus water (No Template Control) in triplicate onto a PCR plate using the suggested volumes.
Step 2. Combine appropriate volumes of PACE Genotyping Master Mix with PACE Genotyping Assay in a tube, as directed, then mix.
Step 3. Dispense the combined mixtures into each of the wells containing DNA using volumes indicated. Each test now contains a complete PACE Genotyping Reaction.
Step 4. Seal your PCR plate with an optically clear seal and centrifuge to ensure all components are at the bottom of the wells.
Step 5.Thermally cycle the reaction plate using the thermal cycling conditions provided.
Step 6. Read the plate and compare data produced with the expected results provided in the manual. Simple!
PACE MECHANISM
More information on the PACE genotyping chemistry and how it works can be found here: www.3crbio.com/#pace. PACE allele-specific PCR is used for the detection of SNPs, Indels and other sequence variants.
REQUIRED COMPONENTS
qPCR machine or Thermocycler + Fluorescent plate reader
PCR plate or equivalent and appropriate optically clear seal
PCR-grade water
Other Products
Aspergillus fumigatus – IgG ELISA
Product Info
Document
Product Info
Name of Product
Aspergillus fumigatus – IgG ELISA
Catalog Number
AF 6100
Short Info
This ELISA kit is intended for the quantitative detection of IgG antibodies against Aspergillus fumigatus in human Serum.
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Method/Platform
ELISA in microplate format
Range/Assay Sensivity
pNPP, λ=405 nm
Test Principle
Aspergillus fumigatus specific antibodies in the sample bind to Aspergillus fumigatus antigens sensitized on microtiter plates. The presence of fungal specific antibodies is detected with a Protein A – alkaline phosphatase conjugate.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Document
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
150 reactions
The HiPure Plant RNA Midi Kit provides fast purification of high-quality RNA from plants, cell, tissues, and yeast using silica-membrane spin columns with a binding capacity of 1mg RNA. There is no need for phenol / chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from <1g simple plant sample without chloroform
Applications
RT-PCR, qPCR, Northern hybridization, second generation sequencing, nucleic acid protection, in vitro translation
Purification method
Midi spin column
Purification technology
Silica technology, DNA filtration technology
Process method
Manual (centrifugation or vacuum)
Sample type
Plant leaves, roots, stems, fruits, fungi, etc.
Sample amount
0.5-1 g
Elution volume
≥400μl
Time per run
≤40 minutes
Liquid carrying volume per column
4ml
Binding yield of column
1mg
Principle
The Kit isolates total RNA from up to 1g plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 40 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to a RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 400µl water using the Kit.
Advantages
Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
High quality – high purity total RNA can be directly used in various sensitive downstream applications
Fast – medium isolation of several samples can be completed in 40 minutes
Safe – no phenol chloroform extraction required
Sensitive – RNA can be recovered at the level of PG
Broad spectrum – various types of plant samples can be processed by diversity of operating procedures
Kit Contents
Contents
R415202
D415203
Purification Times
20 Preps
100 Preps
HiPure DNA Midi Column II
20
100
HiPure RNA Midi Columns II
20
100
15ml Collection Tubes
40
200
Buffer RLC
120 ml
550 ml
Buffer PRC1
120 ml
550 ml
Buffer RW1
70 ml
400 ml
Buffer RW2*
20 ml
100 ml
RNase Free Water
30 ml
120 ml
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Make sure that all buffers are at room temperature when used. During shipment, crystals or precipitation may form in the Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.
Purchase Guide
1. When dealing with woody or uncommon samples, R4150 is recommended first. R4150 contains two polysaccharide/polyphenol lysis buffer, which is the most universal product.
2. R4151 is recommended for handling common economic crop samples for the first time. Strong lysis solution can be used to process easy-extraction samples. The amount of corn or rice leaves samples can reach up to 300mg.
3. R4165 adopts CTAB/chloroform method, which can also handle a large number of difficult-to-extraction plants, but requires contact with chloroform substitutes, which is less safe than other kits. This kit uses DNase Ⅰ to remove DNA, which is also a good choice for extracting polysaccharide/polyphenol-rich plant samples.
4. R4014 is recommended for fruit/starch plant samples, which uses improved trizol pre-treatment, single column operation and is more economical.
Select the right purification kit to get impactful results：
The HiPure Plant RNA Midi Kit provides fast purification of high-quality RNA from plants, cell, tissues, and yeast using silica-membrane spin columns with a binding capacity of 1mg RNA. There is no need for phenol / chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
