Overview
- Fast, reproducible and easy processing of samples
- High yield and high quality genomic DNA with no RNA or protein contamination
- DNA ready for any application including PCR, qPCR, genotyping and more
Norgen’s Cells and Tissue DNA Isolation Kits are designed for the rapid preparation of genomic DNA from cultured cells as well as various tissue samples and urine. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with PCR and Southern Blot analysis.
Cells and Tissue DNA Isolation Kit (Spin Column)
Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The protocol can be completed in approximately 30 minutes for cells and within 90 minutes for tissues. Each kit contains sufficient materials for 50 preparations.
Cells and Tissue DNA Isolation Micro Kit (Micro)
Optimized for small inputs of cells and tissues, such as Laser-Captured Microdissection (LCM). Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. Preparation time for a single sample is approximately 60 minutes, and each kit contains sufficient materials for 50 preparations.
Cells and Tissue DNA Isolation Kit (Magnetic Bead System)
Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. Preparation for 10 purifications is approximately 40 minutes of hands-on time.
Cells and Tissue DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. The Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) also can be integrated with a robotic automation system.
Details
Supporting Data
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| Kit Specifications | |
| Maximum Cells and Tissue Input | 20 mg of animal tissue 3 x 106 cells |
| Average Yield* | 8-10 µg (20 mg of animal tissue) 8-12 µg (3 x 106 cells) |
| Average Purity (OD260/280) | 1.8 – 1.9 |
| Time to Complete 10 Purifications | 40 minutes hands-on time (Cat. 59100) 50 minutes hands on time (Cat. 62500) |
* Average DNA yield will vary depending on the donor
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). This kit is stable for 1 year after the date of shipment.
| Component | Cat. 53100 (50 preps) | Cat. 57300 (50 preps) | Cat. 59100 (50 preps) | Cat. 62500 (192 preps) |
|---|---|---|---|---|
| Lysis Buffer B | 20 mL | 20 mL | 20 mL | 1 x 40 mL 1 x 20 mL |
| Solution WN | 18 mL | 18 mL | 18 mL | 55 mL |
| Wash Solution A | 18 mL | 18 mL | – | – |
| Elution Buffer B | 30 mL | 8 mL | 15 mL | 1 x 30 mL 1 x 15 mL |
| Proteinase K | 1.2 mL | 1.2 mL | 1.2 mL | – |
| Proteinase K in Sotrage Buffer | – | – | – | 4 mL |
| Magnetic Bead Suspension | – | – | 2 x 1.1 mL | 8.5 mL |
| 96-Well Plate | – | – | – | 2 |
| Spin Columns | 50 | – | – | – |
| Micro Spin Columns | – | 50 | – | – |
| Collection Tubes | 50 | 50 | – | – |
| Elution Tubes (1.7 mL) | 50 | 50 | 50 | – |
| 96-Well Elution Plate | – | – | – | 2 |
| Adhesive Tape | – | – | – | 2 |
| Product Insert | 1 | 1 | 1 | 1 |
