Note: Price not include shipment & duty, contact us to get full quote.
The resDNASEQ CHO Residual DNA Quantitation kit is designed for the quantification of residual DNA from CHO, in cell lines which are used for production of biopharmaceutical products. The Ducky Bio residual DNA CHO Assay, based on proven real-time qPCR technology, makes testing of residual DNA from the Chinese hamster ovary (CHO) cell line rapid, specifc. The PCR-based assay is sensitive and specific for DNA from the CHO cell line and not subject to detection of human or environmental DNA that might be introduced during sample handling. The kit was developed to meet the sensitivity requirements defined by WHO (10 ng CHO DNA per therapeutic dose).
Detail
Description
Features of the resDNASEQ CHO Residual DNA Quantitation kit include:
Simpler and Rapid
Only three steps will be need for Sample Preparation and All components of the Sample Preparation Kit can be stored at room temperature.
Only one Reagent for qPCR;
Only 1.5 hours will be needed for the whole test.
Accurate
Perfect amplification curve, good amplification efficiency and good precision.
Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology.
Limit of Detection (LOD): 0.01 fg/μL; Limit of Quantification (LOQ): 0.3 fg/μL.
The recovery rate of different concentration samples in the linear range is between 70% and 130%.
Kit Performance
Fig 1. Only three steps will be need for Sample Preparation and only 20 minitutes will be taken for Sample Preparation.
Fig 2. Seven concentration samples of 0.3fg/μL, 3fg/μL, 30fg/μL, 300fg/μL, 3pg/μL, 30pg/μL, 300pg/μL were detected. CV of each concentration was < 30%, Regression coefficient associated with standard solutions was 0.99992, and amplification efficiency was 100.370%.
Fig 3. Five concentration samples of 0.1 fg/μL, 0.3 fg/μL, 0.5 fg/μL, 1 fg/μL and 3 fg/μL were detected, and 10 multiple wells were detected for each concentration. The CV of concentrati on values of samples with 0.3 fg/μL and above concentrations were less than 30%.
Fig 4. DNA recovery can be determined by including samples spiked with known DNA amounts which are prepared from the corresponding DNA standards. Typically, the range for this value varies from 70% to 130%.
Fig 5. Only one Reagent for qPCR MIX.
Other Products
TCO-PEG24-DBCO
Product Info
Document
Product Info
TCO-PEG24-DBCO is a monodisperse, long PEG linker featuring a trans-cyclooctene and a DBCO group. DBCO easily reacts with azides through click chemistry, while the TCO group readily reacts with tetrazine-containing compounds.
Document
TCO-PEG24-DBCO is a monodisperse, long PEG linker featuring a trans-cyclooctene and a DBCO group. DBCO easily reacts with azides through click chemistry, while the TCO group readily reacts with tetrazine-containing compounds.
Silicone oil thermal conductive shelf, high-precision processing method of upper and lower clamping, shelf temperature uniformity can reach ±0.5°c
Integrated cavity:
Integrated chamber, the shelf and cold trap are located in the same chamber, the steam conduction is faster and the freeze-drying effect is better
Refrigerating and heating control system:
Using thermally conductive silicone oil with a high temperature range and precise PID control algorithm, the control accuracy can reach ±0.5°C. Sample shelf temperature range: -70°C~ +80°C
Freeze-drying endpoint testing system:
The pressure comparison method is used to determine the freeze-drying end point. The freeze-drying end point test can be automatically performed after the analytical drying stage to ensure that the moisture content of the material reaches the standard requirements.
Vacuum precise control and adjustment system:
The sublimation and analytical drying processes are controlled and adjusted with high precision to make the freeze-drying process warmer and the sample sublimation more reliable.
Pulse backfill system:
Three backfilling modes can be selected: slow, medium and fast, which avoids the problem of effective substances being blown away and lost due to the aeration of granular and flocculent materials after freeze-drying.
Manual automatic mode selection:
Manual mode is used to explore process parameters (strong human intervention), automatic mode is used in the mature stage of the process, one-click operation, simple and convenient
Data recording and analysis system:
Through the PC host computer, all operating parameters and background parameters of the equipment can be recorded in real time, and all parameter action changes and action changes of operating components can be recorded.
Freeze-drying process recipe storage function:
The host can store 60 sets of fixed or user-defined freeze-drying process recipes, and the PC can store >10,000 programs (depending on the computer hard drive)
Eutectic point test function:
The eutectic point temperature of the product can be tested offline and online, and the spectrum can be analyzed manually or automatically (optional)
Remote control system:
The operating status of the device can be monitored remotely, and the device can be monitored in real time via WiFi or the cloud.
Mobile phone monitoring system:
You can monitor the operating status of the equipment with your mobile phone, which is simple and convenient (optional)
Cold trap defrost system:
The hot air defrosting method has high safety performance; the defrosting speed is fast, which solves the inconvenience caused by traditional immersion defrosting.
The HiPure Fruit RNA Mini Kit provides fast purification of high-quality RNA from fruits, cell, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100µg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA(mRNA) purification, nuclease protection and in vitro translation.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from <150 mg fruit or seed using chloroform and Plantzol reagent
Applications
RT-PCR, qPCR, Northern hybridization, Poly A purification, nucleic acid protection and in vitro translation translation
Purification method
Mini spin column
Purification technology
Silica technology, DNA filtration technology
Process method
Manual (centrifugation or vacuum)
Sample type
Simple plant, petal, peel, seed
Sample amount
50-150mg
Elution volume
≥300μl
Time per run
≤15 minutes（1-24 samples）
Liquid carrying volume per column
800µl
Binding yield of column
100µg
Principle
The Kit isolates total RNA from up to 150mg plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 15 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 300µl water using the Kit.
Advantages
Efficient DNA removal – one step RNA extraction can effectively remove genomic DNA
High quality – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
Fast – several samples can be extracted in 15 minutes by column method
Wide range – samples including animals, plants, bacteria, cells, etc
High output – large sample amount, and the yield can be up to 200μg
Kit Contents
Contents
R401402
D401403
Purification Times
50 Preps
250 Preps
HiPure RNA Mini Columns
50
250
2ml Collection Tubes
50
250
PlantZol Reagent
60 ml
200 ml
Buffer GXP
30 ml
150 ml
Buffer RW1
50 ml
200 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml
Storage and Stability
The Kit can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. During shipment, crystals or precipitation may form in the Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.
Purchase Guide
1. When dealing with woody or uncommon samples, R4150 is recommended first. R4150 contains two polysaccharide/polyphenol lysis buffer, which is the most universal product.
2. R4151 is recommended for handling common economic crop samples for the first time. Strong lysis solution can be used to process easy-extraction samples. The amount of corn or rice leaves samples can reach up to 300mg.
3. R4165 adopts CTAB/chloroform method, which can also handle a large number of difficult-to-extraction plants, but requires contact with chloroform substitutes, which is less safe than other kits. This kit uses DNase Ⅰ to remove DNA, which is also a good choice for extracting polysaccharide/polyphenol-rich plant samples.
4. R4014 is recommended for fruit/starch plant samples, which uses improved trizol pre-treatment, single column operation and is more economical.
Document
The HiPure Fruit RNA Mini Kit provides fast purification of high-quality RNA from fruits, cell, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100µg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA(mRNA) purification, nuclease protection and in vitro translation.
