Opentrons Flex Prep

TD6 Table top laboratory centrifuge

TD6 Features:

1. Widely used in hospital, laboratory and blood bank.

2. Brushless DC motor for model TD6 which in great torque, free maintenance, no powder pollution, quick in speed up and down.

3. The flexible axle driven system which drive the rotor directly, smooth in operation, low noise and small vibration.

4. Micro computer control system, digital display the RCF、time and speed. There are 10 kinds of program and 10 kinds of acceleration and deceleration for your choice.

5. Electric lid lock, compact design, super speed and imbalance protection.

6. The centrifuge body is made of high-quality steel, safe and reliable.

TD6 Technical Parameters:

Max speed	6000rpm	Max RCF：	4430xg
Max volume	6x100ml	Noise：	≤55dBA
Timer	0～99min	Net weight：	32KG
Dimension(HxDxW)：	540×370×280mm	Power supply	AC 110/220V 50HZ/60hz
Speed accuracy	±20rpm	Acc/Dec	10 Kinds
Certifications	CE, ISO & Calibration report is available.	Warranty	1 Year

Matched Rotors for TD6:

Order No.	Rotor	Max Speed   (rpm)	Max Volume(ml)	Max. RCF(×g）
D6-1	Angle Rotor	6000	6×15/10/7/5ml	3660
D6-2	Angle Rotor	6000	12×15/10/7/5ml	4430
D6-3	Angle Rotor	6000	6×50ml	4110
D6-4	Angle Rotor	6000	4×50ml	3620
D6-5	Angle Rotor	6000	4×100ml	3790
D6-6	Angle Rotor	5000	24×15/10/7/5ml	3500
D6-7	Angle Rotor	4000	12×50ml	2470
D6-8	Angle Rotor	5000	6×100ml	3130
D6-9	Swing Rotor	5000	4x100ml	4420
D6-10	Swing Rotor	5000	4x50ml	4420
D6-11	Swing Rotor	5000	4x4x15ml	2820
D6-12	Swing Rotor	4000	4x6x10ml	2580
D6-13	Swing Rotor	4000	4x4x10ml	2580
D6-14	Microplate	4000	2x2x96well	2020

Overview

• Detection kits for Bacillus cereus
• CE-IVD marked version available for in vitro diagnostic use
• Available in TaqMan format for analysis

Bacillus cereus is a rod-shaped, gram-positive bacterium. Some strains of B. cereus cause foodpoisoning and other diseases such as keratitis. B. cereus grows at a wide range of temperature, from 4°C to 37°C. In fact, many foodborne illnesses caused by B. cereus are a consequence of improperly cooked or improperly stored food, including dairy products and meats. The majority of food poisoning by B. cereus could be divided into two types of symptoms – the emetic type and the diarrheal type, both of which are caused by the enterotoxin produced by the bacteria.

Bacillus cereus TaqMan PCR Kit,
100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Bacillus cereus TaqMan PCR Probe/Primer Set and Controls,
100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

Component	Cat. TM36950 (100 preps)	Cat. TM36910 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
Bacillus cereus Primer & Probe Mix	280 μL	280 μL
Bacillus cereus Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Overview

• Purification and enrichment of intact exosomes from plasma, serum, urine, cell culture media and saliva in less than 30 minutes.
• Versatile sample input ranging from 250 µL to 30 mL
  • Urine Exosome Purification Mini Kit (250 µL – 1 mL urine)
  • Urine Exosome Purification Midi Kit (2 mL – 10 mL urine)
  • Urine Exosome Purification Maxi Kit (11 mL – 30 mL urine)
• Exosome purification is based on Norgen’s proprietary resin separating matrix through exosomes’ surface proteins.
• No precipitation reagents, overnight incubation, protease or coagulant treatments required
• No time-consuming ultracentrifugation, filtration or special syringes required
• Purify intact exosomes with a size ranging from 40-200 nm depending on sample input type
• Purified exosomes are compatible with functional studies.
• Purified exosomes are free from any protein-bound cell-free circulating RNA
• Purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration.
• Exosomal RNA can be extracted from the purified exosomes using Norgen’s Exosomal RNA Purification technology or any other RNA extraction method.

The Urine Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for pure intact exosomes from different urine sample volumes ranging from 250 µL to 30 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different urine sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin.

These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression.

NanoSight® Analysis

Exosomes enriched with Norgen’s Urine Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration.

Exosomal RNA Analysis

Exosomal RNA can be isolated using Norgen’s Exosomal RNA Isolation Kit from exosomes enriched using Norgen’s Urine Exosome Purification Kits for gene expression analysis using RT-qPCR, microarray or NGS and for Biomarker discovery.

Details

Supporting Data

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Kit Specifications
Urine Input (Cat. 57700)	250 μL – 1 mL
Urine Input (Cat. 57800)	2 mL – 10 mL
Urine Input (Cat. 57900)	11 mL – 30 mL
Size of Exosomes Purified	40 nm – 150 nm
Elution Volume	Variable depending on the urine input volume
Time to Complete 10 Purifications	15 – 30 minutes

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

Component	Cat. 57700 (50 preps)	Cat. 57800 (25 preps)	Cat. 57900 (15 preps)
Slurry E	12.5 mL	12.5 mL	12.5 mL
ExoC Buffer	8 mL	30 mL	50 mL
ExoR Buffer	12 mL	12 mL	12 mL
Mini Filter Spin Columns inserted into 2 mL tubes	50	25	15
Product Insert	1	1	1