The Opentrons Flex 96-Channel pipette enables accurate high throughput pipetting, optimized for working in 96-well plates. Flex Pipettes use air displacement technology to offer highly accurate pipetting with pipette volume ranges from 5 – 1000 μL.
Smart sensors support automatic calibration, real-time positioning, and error detection. The 96-channel pipette occupies both pipette mounts and can easily be removed and swapped to an alternate pipette configurations to adapt your workflow needs.
Four 96-Channel Tip Rack Adapters are included with the purchase of one Flex 96-Channel Pipette.
Detail
The Opentrons Flex 96-Channel pipette enables accurate high throughput pipetting, optimized for working in 96-well plates. Flex Pipettes use air displacement technology to offer highly accurate pipetting with pipette volume ranges from 5 – 1000 μL.
Smart sensors support automatic calibration, real-time positioning, and error detection. The 96-channel pipette occupies both pipette mounts and can easily be removed and swapped to an alternate pipette configurations to adapt your workflow needs.
Four 96-Channel Tip Rack Adapters are included with the purchase of one Flex 96-Channel Pipette.
The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.
Mixed linkage β-glucanase (endo-1,3:1,4-β-glucanase) / lichenase (EC 3.2.1.73) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).
Note that the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g. Trichoderma sp.) but this does not result in an increase in absorbance.
Discover more assay kits for enzyme activity measurement.
Data calculators are located in the Documents tab.
Advantages
Very cost effective
All reagents stable for > 2 years
Specific for endo-1,3:1,4-β-glucanase/lichenase
Simple, convenient, rapid assay
Well suited to automation
Malt flour standard and lichenase standard included
Document
The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.
Introducing the Fastin Assay Kit: Your Straightforward Solution for Elastin Quantification! Our user-friendly kit utilizes a dye-based method to measure elastin from in-vivo and in-vitro sources. It can be used to quantify various elastin forms, spanning from immature tropoelastin to mature, ‘insoluble’ elastin fibers.
Colorimetric Detection (513nm) (Endpoint)
Understanding Elastin: The Key to Tissue Flexibility
Tissues like lungs and arteries must maintain the ability to stretch and recoil repeatedly throughout an organism’s life. Elastin, a mature protein, is responsible for this elasticity and is usually present as insoluble fibers within the ECM. During development, these fibers are initially formed from a soluble precursor called tropoelastin.
What is the Fastin Assay?
The Biocolor Fastin assay is a user-friendly, dye-based means of quantifying elastins derived from both in-vivo and in-vitro sources. A variety of elastin forms can be assayed, from immature tropoelastin to mature ‘insoluble’ elastin fibres.
Further information on how the assay works can be found on the ‘Mode of Action‘ tab.
A list of suggested sample types can be found under the ‘Assay Specification‘ tab.
How does the Fastin assay detect Elastin?
The Fastin Dye Reagent contains an elastin-binding synthetic porphyrin, TPPS (5,10,15,20-tetraphenyl- 21H,23H-porphine). This affinity of TPPS for elastin was first observed when used as a ‘vital stain’ on live animals. Most tissues took up the dye initially but only elastin retained the TPPS molecules over time. [Winkelman, J. (1962), Cancer Res. 22, 589-596; Winkelman, J & Spicer, S. (1962), Stain Technol. 37, 303-305].
It has been proposed that the elastin binding of TPPS may be due to the retention of the acidic dye (which contains four charged sulfate groups) by the basic amino acid side chain residues of elastin.
How does the Fastin assay work?
Step 1. Incubation of samples containing soluble elastin with the Fastin Dye Reagent causes an elastin-dye complex to form. This insoluble complex then precipiates.
Step 2. Dye-labelled elastin is then isolated by centrifugation and the unbound dye removed. Elastin-bound dye is then eluted and measured spectrophotometrically.
Step 3. The elastin content of unknown samples can be calculated by comparison against a calibration curve prepared using a standard comprising water-soluble elastin (supplied with the kit).
Assay range
50 – 500µg/ml
Limit of Detection
50µg/ml
Detection Method
Colorimetric Detection (513nm) (Endpoint)
Measurements per kit
110 in total (allows a maximum of 48 samples to be run in duplicate alongside a standard curve).
Suitable Samples
In-vivo: tissues and fluids. Insoluble elastin will first require conversion to water soluble α-elastin using the oxalic acid reagents and extraction protocol supplied with the kit.
In-vitro: Elastin produced by cells during 2D/3D cell culture. NB elastin in conditioned cell media is typically below the detection limit of the kit.
Precautions
This kit is designed for research use only. Not for use in diagnostic procedures. Kit requires access to a centrifuge, heated water bath or block, as well as a spectrophotometer or colorimeter capable of absorbance detection at 513nm. Specific sample preparation protocols may require customer to provide further reagents, consult assay manual for further information.
Fastin elastin kit contents:
1. Dye Reagent (1x110ml)
2. α-elastin Reference Standard (1x5ml, 1.0 mg/ml soluble Bovine elastin)
6. 1.5ml micro-centrifuge tubes for dye-labelling reaction.
7. Assay kit manual
NB: Additional reagents may be required for sample preparation prior to assay. Consult manual or contact us for further details.
Document
Introducing the Fastin Assay Kit: Your Straightforward Solution for Elastin Quantification! Our user-friendly kit utilizes a dye-based method to measure elastin from in-vivo and in-vitro sources. It can be used to quantify various elastin forms, spanning from immature tropoelastin to mature, ‘insoluble’ elastin fibers. Colorimetric Detection (513nm) (Endpoint)
