Opentrons Flex Magnetic Block (GEN 1) is a 96-well magnetic well plate holder containing static ring magnets, specifically designed for use with the Flex Robot. Use it with the Flex Gripper and skirted 96-well PCR or deep-well plates to facilitate quick and efficient separation using magnetic beads. This module is not compatible with the OT-2.
Opentrons Flex Magnetic Block (GEN 1) is a 96-well magnetic well plate holder containing static ring magnets, specifically designed for use with the Flex Robot. Use it with the Flex Gripper and skirted 96-well PCR or deep-well plates to facilitate quick and efficient separation using magnetic beads. This module is not compatible with the OT-2.
Norgen’s EXTRAClean Urine Cell-Free Circulating RNA Purification Kits provide a fast, reliable, reproducible, and simple procedure for isolating circulating RNA and exosomal RNA from various urine inputs ranging from 250 μL and up to 30 mL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extensions, and expression array assays.
EXTRAClean Cell-Free Circulating RNA Purification Mini Kit:
For sample volumes ranging from 250 μL to 2 mL
EXTRAClean Cell-Free Circulating RNA Purification Midi Kit:
For sample volumes ranging from 2mL to 10mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL
EXTRAClean Cell-Free Circulating RNA Purification Maxi Kit:
For sample volumes ranging from 10 mL to 30 mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL.
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| Kit Specifications | |
|---|---|
| Sample Type | Fresh or frozen urine |
| Sample Volume Range | 250 μL to 2mL |
| Minimum Elution Volume | 50 μL |
| Maximum Elution Volume | 100 μL |
| Time to Complete 10 Purifications | 25–30 minutes |
| Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
| Average Yields ¥ | Variable depending on specimen |
¥Please check page 5 of protocol for average urine yields and common RNA quantification methods.
All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
| Component | Mini (Cat. 72900) | Midi (Cat. 72910) | Maxi (Cat. 72920) |
|---|---|---|---|
| Binding Solution K | 25 mL | 75 mL | 1 x 75 mL 1 x 25 mL |
| Lysis Buffer A | 30 mL | 20 mL | 20 mL |
| Wash Solution A | 18 mL | 18 mL | 18 mL |
| Elution Solution A | 6 mL | 6 mL | 6 mL |
| Collection Tubes | 50 | 20 | 10 |
| Elution Tubes (1.7 mL) | 50 | 20 | 10 |
| EXTRAClean Mini Spin Columns | 50 | 20 | 10 |
| EXTRAClean Midi Spin Columns | – | 20 | – |
| EXTRAClean Maxi Spin Columns | – | – | 10 |
| Product Insert | 1 | 1 | 1 |
HiPure FFPE Nucleic acid Kit supplies a simple and rapid DNA extraction for formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 20 minutes (not including digestion time). DNA can be directly used for downstream applications such as PCR, southern blot and viral DNA detection, etc.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from FFPE tissue samples |
| Applications | PCR, southern blot and viral DNA detection, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples |
| Sample amount | <20mg |
| Elution volume | >15µl |
| Time per run | ≤20 minutes |
| Liquid carrying volume per column | 4ml |
| Binding yield of column | 100μg |
Hipure FFPE Nuclear acid kit adopts silica gel column purification. The sample is deparaffinated by xylene and digested by lysate and protease. After de crosslinked at 90 ℃, DNA/RNA is released into the lysate. Adding ethanol to adjust the binding conditions, the sample is transferred to the column where DNA/RNA is adsorbed on the membrane and protein is removed without adsorption. Protein and other impurities are washed by buffer GW1, and the salt is removed by buffer GW2. Finally, the DNA / RNA is eluted by low salt buffer.
| Contents | D312602 | D312603 |
| Purification Times | 50 Preps | 250 Preps |
| HiPure DNA Mini Columns I | 50 | 250 |
| 2ml Collection Tubes | 50 | 250 |
| Buffer DPS | 30 ml | 150 ml |
| Buffer ATL | 15 ml | 60 ml |
| Buffer AL | 15 ml | 60 ml |
| Buffer GW1* | 22 ml | 88 ml |
| Buffer GW2* | 12 ml | 50 ml |
| Proteinase K | 24 mg | 120 mg |
| Protease Dissolve Buffer | 1.8 ml | 10 ml |
| Buffer AE | 10 ml | 30 ml |
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Experiment Data
HiPure FFPE Nucleic acid Kit supplies a simple and rapid DNA extraction for formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 20 minutes (not including digestion time). DNA can be directly used for downstream applications such as PCR, southern blot and viral DNA detection, etc.
DDL6 4x1000ml Swing rotor for 4x500ml Blood Bags Refrigerated Centrifuge
DDL6 Blood Bags Refrigerated Centrifuge Application:
DDL6 Refrigerated centrifuge widely used in the field of blood bank station, pharmaceutical factory and laboratory, can hold 500ml triple, 400ml triple and 250ml triple blood bags.
DDL6 Blood Bags Refrigerated Centrifuge Features:
1. Brushless frequency motor which in great torque, free maintenance, no powder pollution, quick in speed up and down.
2. Digital display which indicates the program, speed, time, RCF and temperature.
3. Micro computer control, there are 10 kinds of program and 10 kinds of acceleration/deceleration for your choice.
4. There are 6 kinds of rotors for your choice.
5. Electric lid lock, compact design, super speed and imbalance protection.
6. The centrifuge body is made of high-quality steel, safe and reliable.
DDL6 Blood Bags Refrigerated Centrifuge Technical Parameters:
| Max Speed:6000r/min | Max RCF:6600g |
| Max Volume；4x1000ml | Power supply:AC220V,50HZ,15A |
| Timer:1~9h/59min | Noise:≤56dBA |
| Dimension:800x675x830mm | Net Weight:260KG |
| Speed Accuracy:±20r/min | Package (W*D*H):920x800x1320mm |
| Temperature range: | -20℃ ~ 40℃ |
DDL6 Blood Bags Refrigerated Centrifuge Matched Rotors:
| Rotor No. | Rotor type | Max.Speed (rpm) | Max.Volume (ml) | Max.RCF (xg) |
| No33097 | Swing out rotor | 4,000 | 4x1000ml (round/square cup ) | 4,060 |
| No30221 | Angle rotor | 6,000 | 4x300ml | 5,390 |
| No30222 | Angle rotor | 6,000 | 6x300ml | 5,660 |
| No30223 | Angle rotor | 6,000 | 6x500ml | 6,600 |
The Corners of our Company____________________________________
Metal housing with 3 layers protection. Quiet noise, Less vibration & Fast cooling guarantee a perfect separation.
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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