The Opentrons Temperature Module, compatible with both the OT-2 and Flex Robots, is a fully automated thermoelectric module and integrated for use with the Opentrons platform. It is used to achieve temperature changes ranging from 4 to 95°C for various applications.
The Temperature Module GEN2 is compatible with the new Opentrons Flex Robot when paired with the Flex Temperature Caddy and Calibration Adapter. The caddy sits below the deck to enable below-deck cable routing and labware placed on modules remain closer to the deck surface. The calibration adapter is used to calibrate the location of the module. If you choose to include the Opentrons Flex Caddy and Calibration Adapter, your order will ship in 20 business days.
Detail
The Opentrons Temperature Module, compatible with both the OT-2 and Flex Robots, is a fully automated thermoelectric module and integrated for use with the Opentrons platform. It is used to achieve temperature changes ranging from 4 to 95°C for various applications.
The Temperature Module GEN2 is compatible with the new Opentrons Flex Robot when paired with the Flex Temperature Caddy and Calibration Adapter. The caddy sits below the deck to enable below-deck cable routing and labware placed on modules remain closer to the deck surface. The calibration adapter is used to calibrate the location of the module. If you choose to include the Opentrons Flex Caddy and Calibration Adapter, your order will ship in 20 business days.
Other Products
Urine DNA Isolation Kit for Exfoliated Cells or Bacteria
Product Info
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Product Info
Overview
Isolate genomic DNA from either exfoliated cells or bacteria found in urine
Isolate and detect genomic DNA from as little as 1 mL of urine and up to 50 mL urine
High quality DNA for sensitive applications
Rapid processing time
Norgen’s Urine DNA Isolation Kit for Exfoliated Cells or Bacteria is designed for the rapid isolation of either: 1) human genomic DNA from exfoliated cells that have been shed into the urine from the urinary tract; or 2) bacterial genomic DNA from urine samples. The kit allows for the isolation of DNA from 1 to 50 mL of urine. The genomic DNA isolated from exfoliated cells can be used in a number of diagnostic and research applications including the diagnosis and monitoring of bladder, kidney, or other urinary-tract cancers. Bacterial genomic DNA from both human urine samples and urine samples from animals can be isolated with this kit in order to study the levels and types of bacteria that are present. The kit allows for the isolation of genomic DNA from both Gram negative and Gram positive bacteria, including E. coli, Proteus spp., Klebsiella spp., Enterobacter spp., Serratia spp., Pseudomonas spp, Clostridial ssp. and Leptospirosis spp.Chlamydia trachomatis and Neisseria gonorrhoeae.
Typical yields of human genomic DNA from exfoliated cells will vary depending on the cell density of the urine sample, which is affected by a number of factors including health, diet and sex of the individual donating the urine. Typical yields of bacterial genomic DNA will vary depending on the urine sample and the bacterial species, if any, present in the urine. Healthy humans generally have < 10, 000 CFU of bacteria per mL of urine, and this kit is sufficiently sensitive to isolate and detect DNA from even this minimal amount of bacteria. The genomic DNA purified with this kit is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications such as PCR, qPCR and Southern Blot analysis.
10 minutes (Plus a 30 minute incubation – Bacteria) (Plus a 15 minute incubation – Exfoliated)
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.
500 Lateral Flow Cassettes (top and bottom) Cassette Lettering: CT Fits strips that are 86 mm x 6 mm Perfect for development and product launch Large quantities available
Solid Phase Reversible Immobilization (SPRI) beads are a simple and effective reagent for DNA purification, but not for short oligo purification. The beads are paramagnetic particles coated with carboxyl groups that can reversibly bind to nucleic acid. However, SPRI beads can only purify DNA/RNA fragments that are 100 base pairs or longer. DNA/RNA fragments shorter than 100 base pairs are not effectively recovered.
Oligo purification can also be performed using spin column-based technology. The oligo size limitation for recovery is around 20 nt, as oligos under 20 nt have a very low recovery rate.
We have developed Magnetic Beads (Short Oligo Purification) Kit for short oligo purification. Our proprietary bead technology enables the recovery of oligos as short as 6 nt. 80% of the 6 nt oligos and 90% of the > 8 nt oligos can be recovered. The reagent also effectively removes impurities and unwanted components such as salts, proteins, dNTPs, detergents, and other contaminants. The magnetic bead reagents are RNase free, and can be used for both DNA and RNA applications.
Recovery rates of of short oligos. Oligos with different sizes were used as input. BioDynami Short Oligo Quantification Kit (Cat. # 40046) was used to quantify the recovery rates with modifications.
Features
Effective purification of short oligos
6 nt oligos: 80% recovery rate
>8 nt oligos: >90% recovery rate
Removal of impurities and unwanted reaction components
Document
Solid Phase Reversible Immobilization (SPRI) beads are a simple and effective reagent for DNA purification, but not for short oligo purification. The beads are paramagnetic particles coated with carboxyl groups that can reversibly bind to nucleic acid. However, SPRI beads can only purify DNA/RNA fragments that are 100 base pairs or longer. DNA/RNA fragments shorter than 100 base pairs are not effectively recovered.
