The NGS DNA Fragmentation & Library Prep Kit (ion torrent platform) was developed for construction of high quality libraries for next generation sequencing for ion torrent platform. The kit uses intact genomic DNA (both EDTA-free DNA and DNA in TE buffer are compatible) as input DNA without an additional DNA fragmentation step. Our NGS kit has a fast and simple workflow with only three step. In addition,  all steps can be performed in one tube. The DNA libraries can be generated within one hour with less than 10 minutes of  hands-on time. Library multiplexing up to 12 samples is possible.

NGS DNA Fragmentation & Library Prep Kit Workflow

The incorporation of DNA fragmentation in the kit makes it possible to directly use intact genomic DNA as input DNA without the need of mechanical DNA shearing or enzymatic DNA fragmentation. The protocol is optimized to generate libraries from 200 bp to 500 bp in size. The library size is inversely correlated with the incubation time of step 1 at 20°C.

Kit features:

• • • Fast: 1-hour library construction from intact genomic DNA to NGS library
    • • Total time: 1 hr
      • Hands-on time: 5 min
    • Intact genomic DNA can be used directly as input; DNA fragmentation is not required.
    • Works with both EDTA-free DNA samples and DNA samples in TE buffer
    • Simple workflow
    • • Three steps
      • Only one beads purification step
    • Guaranteed quality: high library conversion efficiency based on our chemistry

Library conversion efficiency for NGS DNA Fragmentation & Library Prep Kit (Ion Torrent Platform). 100 ng, 300 ng and 500 ng of DNA were used as input.

NGS DNA Fragmentation & Library Prep Kit (Ion Torrent Platform): Library size distribution with different incubation time.

The NGS DNA Fragmentation & Library Prep Kit (ion torrent platform) was developed for construction of high quality libraries for next generation sequencing for ion torrent platform. The kit uses intact genomic DNA (both EDTA-free DNA and DNA in TE buffer are compatible) as input DNA without an additional DNA fragmentation step. Our NGS kit has a fast and simple workflow with only three step. In addition, all steps can be performed in one tube. The DNA libraries can be generated within one hour with less than 10 minutes of hands-on time. Library multiplexing up to 12 samples is possible.

Introduction

MagPure Stool DNA Kit is specially designed for high throughput DNA extraction from stool samples. It can get high purity microbial DNA from stool samples (≤200mg). This kit is based on magnetic beads purification and unique inhibiting factors adsorption technology, no phenol-chloroform extraction or alcohol precipitation. It can adsorb humic acid and other inhibiting factors in the solution efficiently. DNA can be directly used for downstream applications such as PCR, Viral DNA testing, bacterial DNA testing, etc.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from 100-150mg stool samples
Applications	PCR, southern blot and enzyme digestion, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Stool
Sample amount	100-150 mg
Elution volume
Time per run

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

• Fast – several samples can be extracted in 60 minutes (after digestion)
• High purity – unique adsorbent can completely remove inhibitory factors
• High recovery – DNA can be recovered at the level of PG

Kit Contents

Contents	D636401	D636402	D636404
Purification Times	48 Preps	96 Preps	400 Preps
MagPure Particles N	1.7 ml	3.4 ml	14 ml
2ml Bead Tubes	48	96	400
RNase A	10 mg	20 mg	75 mg
Proteinase K	24 mg	48 mg	180 mg
Protease Dissolve Buffer	3 ml	5 ml	20 ml
Buffer ATL	60 ml	110 ml	300 ml
PVP-10	1.2 g	2.2 g	6 g
Buffer PCI	50 ml	100 ml	300 ml
Buffer MLE	30 ml	60 ml	180 ml
Buffer GW1*	22 ml	44 ml	132 ml
Elution Buffer	20 ml	20 ml	60 ml

Storage and Stability

MagPure Particles, RNase A and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

MagPure Stool DNA Kit is specially designed for high throughput DNA extraction from stool samples. It can get high purity microbial DNA from stool samples (≤200mg). This kit is based on magnetic beads purification and unique inhibiting factors adsorption technology, no phenol-chloroform extraction or alcohol precipitation. It can adsorb humic acid and other inhibiting factors in the solution efficiently. DNA can be directly used for downstream applications such as PCR, Viral DNA testing, bacterial DNA testing, etc.

Overview

• Detection kits for Cryptosporidium
• CE-IVDD marked in accordance with the European Commission Directive 98/79/EC.
• Research version available
• Available in Taqman Probe format for analysis

Cryptosporidium is a parasite found in water that causes an infection in mammals termed cryptosporidiosis. It is one of the most common water-borne diseases and is found world-wide. It affects the intestines of mammals and typically causes an acute short-term infection. The most common symptom is self-limiting diarrhea in healthy individuals, however in immunocompromised individuals the symptoms are particularly severe and often fatal. There is no specific treatment for cryptosporidiosis other than fluid rehydration and management of any pain. Therefore early detection of Cryptosporidium in water is the foremost action to prevent the infection.

Cryptosporidium TaqMan RT-PCR Kit, 24 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Details

Supporting Data

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Storage Conditions

The Cryptosporidium TaqMan PCR Kit Dx is shipped on dry ice. The components of the kit should be frozen upon arrival. If one or more of the components is not frozen when the kit is received, or if any of the components have been compromised during shipment, please contact Norgen Biotek for assistance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 3 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. DxTM39100 (24 rxns)
MDx TaqMan 2X RT-PCR Master Mix Dx	550 µL
Cryptosporidium Primer & Probe Mix Dx	2 x 70 µL
Cryptosporidium Positive Control Dx – 200,000 μL	50 µL
Nuclease-Free Water	2 x 1.25 mL
Product Insert	1