Thermal adapter for a range of ANSI/SLAS compliant labware, e.g., Corning 384 Well Plate 112 µL. Compatible with the Opentrons Heater-Shaker Module.
Thermal adapter for a range of ANSI/SLAS compliant labware, e.g., Corning 384 Well Plate 112 µL. Compatible with the Opentrons Heater-Shaker Module.
Thermal adapter for a range of ANSI/SLAS compliant labware, e.g., Corning 384 Well Plate 112 µL. Compatible with the Opentrons Heater-Shaker Module.
This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 3 mL to 25 mL. Both high molecular weight DNA (greater than 1 kb in size; mostly cell associated) and the smaller DNA (150 – 250 bp; derived from the circulation) is effectively isolated and purified with a rapid and convenient spin column protocol. Multiple samples can be processed in 45 minutes. Salts, metabolic wastes, proteins and other contaminants are removed to yield inhibitor-free DNA for use in sensitive applications such as PCR, qPCR, DNA fingerprinting, methylation studies and more. This kit can also be used to isolate DNA from a broad range of viruses.
This kit is fully compatible with Norgen’s Urine Collection and Preservation Tubes.
Norgen’s Urine DNA Isolation Kit Dx (Slurry Format) does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.
NOTE: This product is not available for sale in the United States.
Figure 1 / 3
Click for expanded view
| Kit Specifications – Spin Column | |
| Minimum Urine Input | 3 mL |
| Maximum Urine Input | 25 mL |
| Time to Complete 10 Purifications | < 30 minutes |
| Size of Urine DNA Purified | Large (> 1 kb) and small (150-250 bp) |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. All solutions and plastics can be used until the expiration date specified on their labels. It is recommended to warm Solution A and Solution B for 20 minutes at 60°C if any salt precipitation is observed
| Component | Cat. Dx48800 (50 preps) |
|---|---|
| Solution A | 18 mL |
| Solution B | 30 mL |
| Wash Solution | 22 mL |
| Elution Buffer | 6 mL |
| Mini Filter Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
HiPure FFPE RNA Kit supplies a simple and rapid RNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 30 minutes (not including digestion time). RNA can be directly used for downstream applications such as RT-PCR, Northern blot, vitro translation and other experiments.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from FFPE tissue and section samples |
| Applications | RT-PCR, quantitative RT-PCR, Northern hybridization, Poly A purification, nucleic acid protection and in vitro translation |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | FFPE tissue sample |
| Sample amount | 6mg |
| Yield | 20μg |
| Elution volume | ≥20μl |
| Time per run | ≤60 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 100µg |
This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis with proteinase K digestion requires only 15 minutes. After lysis, samples are incubated at 80ºC for 15 minutes. Transfer to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-salt buffer.
Advantages
Kit Contents
| Contents | R414302 | D414303 |
| Purification Times | 50 Preps | 250 Preps |
| HiPure RNA Micro Columns | 50 | 250 |
| 2ml Collection Tubes | 50 | 250 |
| Buffer DPS | 60 ml | 250 ml |
| Buffer FRL | 15 ml | 60 ml |
| Buffer RLC | 15 ml | 60 ml |
| Buffer RWC* | 10 ml | 50 ml |
| Buffer RW2* | 20 ml | 2 x 50 ml |
| Protease Dissolve Buffer | 1.8 ml | 10 ml |
| Proteinase K | 24 mg | 120 mg |
| RNase Free Water | 10 ml | 20 ml |
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
HiPure FFPE RNA Kit supplies a simple and rapid RNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 30 minutes (not including digestion time). RNA can be directly used for downstream applications such as RT-PCR, Northern blot, vitro translation and other experiments.
The HiPure Plasmid EF Maxi Kit combines the power of HiPure technology with Magen’s innovative endotoxin removal technology (ETR) to deliver high-quality plasmid DNA with low endotoxin levels for use in eukaryotic transfection, and in vitro experiments. Up to 1000 µg high copy number plasmid DNA or 200 µg low copy number plasmid DNA can be purified from 150 mL overnight culture.
F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here
Specifications
| Features | Specifications |
| Main Functions | Isolation up to 1.5mg endotoxin-free plasmid DNA from 200ml bacterial culture |
| Applications | Cell transfection, animal injection, etc. |
| Purification method | Maxi spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Conventional plasmid vector |
| Sample amount | High copy plasmid vector: 100-150ml culture mediumLow copy plasmid vector: 150-200ml culture medium |
| Yield | 200-1500μg |
| Elution volume | ≥0.7ml |
| Time per run | ≤60 minutes |
| Liquid carrying volume per column | 20ml |
| Binding yield of column | 1mg |
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
Kit Contents
| Contents | P115602 | P115603 |
| Purification Times | 10 Preps | 50 Preps |
| RNase A | 30 mg | 150 mg |
| Buffer P1 | 100 ml | 500 ml |
| Buffer P2 | 100 ml | 500 ml |
| Buffer LN3 | 50 ml | 250 ml |
| Buffer PW1 | 33 ml | 180 ml |
| Buffer PW2 | 20 ml | 100 ml |
| Elution Buffer | 15 ml | 120 ml |
| Buffer CL | 33 ml | 180 ml |
| HiPure DNA Maxi Columns C | 10 | 50 |
| Lysate Clear Midi Syringe | 10 | 50 |
| 50 ml Collection Tubes C | 20 | 100 |
Storage and Stability
The kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers,warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2–8°C.
For any technical problems or customized products, please contact us.
F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here
The HiPure Plasmid EF Maxi Kit combines the power of HiPure technology with Magen’s innovative endotoxin removal technology (ETR) to deliver high-quality plasmid DNA with low endotoxin levels for use in eukaryotic transfection, and in vitro experiments. Up to 1000 µg high copy number plasmid DNA or 200 µg low copy number plasmid DNA can be purified from 150 mL overnight culture.