Opentrons Flex Filter Tips, 200 µL

Description 

The PM2800 ExcelBand™ 3-color Extra Range Protein Marker is a ready-to-use three-color protein standard with 13 pre-stained proteins covering an extra range of molecular weights from 10 to 310 kDa in Tris-Glycine buffer (9 to 290 kDa in Bis-Tris (MOPS) buffer and 10 to 290 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for three reference bands (one green and two red bands at 25 kDa and 75, 310 kDa respectively) when separated on SDS-PAGE in Tris-Glycine buffer. The PM2800 ExcelBand™ 3-color Extra Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for approximating the size of proteins. The marker is supplied in the gel loading buffer and is ready to use. Do NOT heat, dilute, or add reducing agents before loading.

Features

• Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.
• Three reference bands — 310 kDa (red), 75 kDa (red) and 25 kDa (green)

Contents

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25℃), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol). 

Quality Control

Under suggested conditions, PM2800 3-color Extra Range Protein Marker resolves 13 major bands in SDS-PAGE (Tris-Glycine buffer, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane. 

Storage

4°C for 3 months
-20°C for long term storage

The PM2800 ExcelBand™ 3-color Extra Range Protein Marker is a ready-to-use three-color protein standard with 13 pre-stained proteins covering an extra range of molecular weights from 10 to 310 kDa in Tris-Glycine buffer (9 to 290 kDa in Bis-Tris (MOPS) buffer and 10 to 290 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for three reference bands (one green and two red bands at 25 kDa and 75, 310 kDa respectively) when separated on SDS-PAGE in Tris-Glycine buffer. The PM2800 ExcelBand™ 3-color Extra Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for approximating the size of proteins. The marker is supplied in the gel loading buffer and is ready to use. Do NOT heat, dilute, or add reducing agents before loading.

Overview

• Convenient optimized on-column DNase treatment using Norgen’s RNA Purification Kits
• Also includes protocol for digestion in-solution followed by RNA Clean-Up
• Guaranteed RNase-Free
• Includes Enzyme Incubation Buffer
• Cat. 25710 contains one vial of 1,600 units and Cat. 25720 contains 4 vials (1,600 units/vial)

Norgen’s RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen’s RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen’s RNA purification kits. Alternatively, after isolating total RNA using one of Norgen’s RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen’s RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.

Details

Each RNase-Free DNase I Kit is supplied complete with sufficient enzyme and enzyme incubation buffer for 50 or 200 reactions.

Storage Conditions
The DNase I provided is in lyophilized form. It is stable for at least 3 months if stored at room temperature. However, it is recommended to store the DNase I vial at 2 – 8ºC (or below) upon receipt to maintain stability beyond 3 months. Buffer DR and Enzyme Incubation Buffer can be stored at room temperature. After reconstitution with Buffer DR (see product manual), the DNase I should be stored at -20ºC. All reagents should remain stable for at least 1 year in their unopened containers at the appropriate storage temperature.

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target Accurate controls to confirm findings