The Opentrons® 3-piece Aluminum Block Set is used with our Temperature Deck. The set comes with a 24-well, 96-well and flat bottom plate format block. These are used to keep your 2mL tubes, 1.5mL tubes, 96-well PCR plates, PCR strips and flat bottom plates at a constant temperature. These blocks holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).
The Opentrons® 3-piece Aluminum Block Set is used with our Temperature Deck. The set comes with a 24-well, 96-well and flat bottom plate format block. These are used to keep your 2mL tubes, 1.5mL tubes, 96-well PCR plates, PCR strips and flat bottom plates at a constant temperature. These blocks holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).
Product Description
Lyophilized RNA Amplification Kit -20°C Format Easy And Efficient
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39 ºC~42 ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands, and binds the auxiliary protein and single strand With the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology position and strand exchange begins; accompanied by the recombinase from the complex Upon dissociation, the polymerase also binds to the 3′ end of the primer, initiating chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
| Parameters | Details |
|---|---|
| Product Name | RNA Isothermal Amplification Kit NFO |
| Manufacturer | Amp-future |
| Storage Temperature | -20°C |
| Kit Components | Enzymes, Buffers ,Reagents |
| Packaging | 48 Tests/box |
| Detection Limit | 500-1000copies/µL |
| Shipping | ICE |
| Test Time | 5-20mins |
Isothermal nucleic acid Applications
Suitable for RNA isothermal rapid amplification kit(NFO type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
RNA NFO kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
E-cadherin is an intercellular adhesion molecule present in epithelial cells. Anti-E-cadherin stains glandular epithelium, as well as lung, gastrointestinal and ovarian adenocarcinomas. A panel of antibodies against E-cadherin and p120 is also used to differentiate ductal (membranous staining) and lobular breast cancer (cytoplasmic staining). Anti-E-cadherin also stains some thyroid cancers.
Attogene Fluorescent Universal Lateral Flow Assay Kits are a convenient ready-to-use kit for quick and cost-effective development of a fluorescent lateral flow dipstick assay for detection of DNA and RNA products.
Fluorescent lateral flow assays can be 2-100 fold more sensitive that gold based lateral flow tests.
Formats (fluorescent broad range UV light excitation range of 100nm to 400nm, 655nm emission) Streptavidin conjugate pad):
• Detectable using a black light such as a black light UV flashlight or fluorescent lateral flow reader.Inquire about custom configurations: [email protected].
For example, this product can be configured with alternative/custom streptavidin fluorescent particles.
Kit Components
Features & Benefits
