The dsDNA Quantification Broad Range Kit and dsDNA Quantification High Sensitivity Kit are developed for double stranded DNA quantification using microplate readers. The DNA Quantification BR kit includes BR Dye, BR Dilution Buffer, and BR DNA Standards. The DNA Quantification HS kit includes HS Dye, HS Dilution Buffer, and HS DNA Standards. Simply dilute the Dye with the Dilution Buffer, add DNA sample, then read the concentration using microplate reader. The BR assay is accurate in the linear range from 4 to 1000 ng and the HS assay is accurate in the linear range from 0.2 to 100 ng. Both kits are highly selective for double-stranded DNA over RNA.
The Quantification Kits have several advantages over traditional DNA quantitation such as sensitivity, stability, and tolerance of contaminants.
Both kits reduce the cost of DNA quantification by 60% as compared to other brands.
Selectivity and sensitivity of the kits
Save 60% of the costs.
The performance of the kit is nearly identical to that of Thermo Fisher’s kit (figure below).
Comparison of BioDynami dsDNA Quantification Broad Range Kit with Thermo Fisher kit.
Comparison of BioDynami dsDNA Quantification High Sensitivity Kit with Thermo Fisher kit.
Common contaminants such as salts, free nucleotides, solvents, detergents, RNA, single stranded DNA, or protein are well tolerated in the assay (Table 1).
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Leukocyte RNA Purification Kits
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Product Info
Overview
Fractionate leukocytes from whole blood in minutes with provided RBC Lysis Buffer
Isolate total RNA, including microRNA, without phenol
Rapid and convenient spin-column format and 96-well plate for high throughput applications
Purified RNA is ready for any downstream application including RT-PCR, qRT-PCR, NGS, arrays and more. Find out more information on Norgen’s NGS services
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
These kits provide a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from mammalian blood samples. These kits are supplied with an RBC (red blood cell) Lysis Buffer for selective removal of red blood cells and fractionation of leukocytes by centrifugation. Isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. These kits are able to isolate total leukocyte RNA, including both large mRNA and all small RNA species containing microRNA (miRNA) and small silencing RNA (siRNA). The purified RNA is of the highest quality and can be used in a number of downstream applications.
Leukocyte RNA Purification Kit (Spin Column)
This kit provides a rapid method for the isolation and purification of total leukocyte RNA from mammalian blood samples in 40 minutes. Allowable blood input ranges from 10 μL to 2 mL or 3 x 106 Leukocytes
Leukocyte RNA Purification Plus Kit (Plus)
Norgen’s Leukocyte RNA Purification Plus Kit provides a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from up to 3 mL of mammalian blood samples. Complete 10 purifications in as little as 40 minutes.
Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Norgen’s kit allows for the isolation of total leukocyte RNA, including all small RNA species. The purified RNA is of the highest quality and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, northern blotting, RNase protection and primer extension, and expression array assays. Allowable blood input ranges from 10 μL to 1 mL or 1.5 x 106 Leukocytes.
Average Yield Human Blood (500 μL) Hamster Blood (100 μL)
1.5 μg 2.5 μg
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The RBC Lysis Buffer should be stored at 4°C upon arrival.
Discover our Modified Tryptone Soya Broth, designed for the enrichment and cultivation of a wide range of microorganisms, ensuring accurate and reliable microbiological testing.
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Discover our Modified Tryptone Soya Broth, designed for the enrichment and cultivation of a wide range of microorganisms, ensuring accurate and reliable microbiological testing.
Carbendazim is a fungicide used for the treatment and control of fungal diseases. Carbendazim continues to be used, frequently in combination with other fungicides.
This kit is based on ELISA technology, which is fast, easy, accurate and sensitive compared with common instrumental analysis and only needs 1.5 hours in one operation, it can considerably minimize operation error and work intensity.
