The NGS DNA Library Prep Kit (Ion Torrent platform) was developed for construction of high quality DNA libraries for next generation sequencing using Ion Torrent platform. The kit uses short double strand DNA fragments (blunt ends and/or sticky ends) as input DNA for NGS library construction, and is compatible with DNA fragments generated from both enzymatic methods (BioDynami DNA Fragmentation Enzyme Mix and DNA Fragmentation & A-tailing Enzyme Mix etc) and mechanical methods (sonication, nebulization etc.). Our unique technology increases library conversion efficiency and eliminates insert concatemer ligation. Library multiplexing up to 12 samples is possible.
Detail
The NGS DNA Library Prep Kit (Ion Torrent platform) was developed for construction of high quality DNA libraries for next generation sequencing using Ion Torrent platform. The kit uses short double strand DNA fragments (blunt ends and/or sticky ends) as input DNA for NGS library construction, and is compatible with DNA fragments generated from both enzymatic methods (BioDynami DNA Fragmentation Enzyme Mix and DNA Fragmentation & A-tailing Enzyme Mix etc) and mechanical methods (sonication, nebulization etc.). Our unique technology increases library conversion efficiency and eliminates insert concatemer ligation. Library multiplexing up to 12 samples is possible.
NGS DNA Library Prep Kit Workflow
Kit features
Simple procedure: Reactions for all three steps are in one tube
Fast protocol
Total protocol time is around 1 hour
Hands-on time is only ~5 minutes
Guaranteed high library conversion efficiency as compared to other kits
Input DNA amount: from 50 ng to 1 ug
No insert concatemer ligation
Other Products
D-Glucose Assay Kit (GOPOD Format)
Product Info
Document
Product Info
K-GLUC
SKU: 700004297
660 assays per kit
Content:
660 assays per kit
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
D-Glucose
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
510
Signal Response:
Increase
Linear Range:
4 to 100 μg of glucose per assay
Limit of Detection:
40 mg/L
Reaction Time (min):
~ 20 min
Application examples:
Wine, beer, fruit juices, soft drinks, milk, jam, dietetic foods, bakery products, candies, fruit and vegetables, tobacco, cosmetics, pharmaceuticals, feed, paper and other materials (e.g. biological cultures, samples, etc.).
Method recognition:
Widely used and accepted in clinical chemistry and food analysis
The D-Glucose test kit contains high purity reagents for the measurement and analysis of D-glucose in cereal extracts and for use in combination with other Megazyme kits.
All reagents stable for > 12 months after preparation
Very competitive price (cost per test)
Simple format
Standard included
Document
The D-Glucose test kit contains high purity reagents for the measurement and analysis of D-glucose in cereal extracts and for use in combination with other Megazyme kits.
Heat-Labile Exonuclease I (HL-ExoI) is a 3’ – 5’ exonuclease, specific for single stranded DNA. The enzyme is recombinantly produced in E. coli. HL-ExoI is active at 25 – 37°C and inactivated by 1 minute incubation at 80°C or 15 minutes at 60°C.
HL-ExoI is used for degradation of ssDNA such as primers and oligos. It is also ideal for treatment of sensitive samples and useful in the development of novel molecular diagnostics applications.
Key Features
3’-5’ exonuclease specific for single stranded DNA
High activity at 25 – 37°C
Easily heat-inactivated by 1 min incubation at 80°C, or 15 min at 60°C
Moderate salt tolerance
Application
Removal of primers post-PCR prior to DNA sequencing or SNP detection
Figures
Properties
Quality Control
ArcticZymes is dedicated to the quality of our products. We manufacture all products at our ISO 13485 certified facility in Norway.
Document
Heat-Labile Exonuclease I (HL-ExoI) is a 3’ – 5’ exonuclease, specific for single stranded DNA. The enzyme is recombinantly produced in E. coli. HL-ExoI is active at 25 – 37°C and inactivated by 1 minute incubation at 80°C or 15 minutes at 60°C.
[PM2600] ExcelBand™ 3-color High Range Protein Marker (9-245 kDa), 250 μl x 2
Product Info
Document
Product Info
Description
The PM2600 ExcelBand™ 3-color High Range Protein Marker is a ready-to-use three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine Buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10 to 235 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer). The PM2600 3-color High Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Features
Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.
Two reference bands — 75 kDa (red) and 25 kDa (green)
Contents
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol).
Quality Control
Under suggested conditions, PM2600 ExcelBand™ 3-color High Range Protein Marker resolves 12 major bands in 15% SDS-PAGE (Tris-Glycine buffer, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane.
Storage
4°C for 3 months -20°C for long term storage
Document
The PM2600 ExcelBand™ 3-color High Range Protein Marker is a ready-to-use three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine Buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10 to 235 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer). The PM2600 3-color High Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
