This kit is sufficient for 150 reactions: For characterizing cyanobacteria in environmental samples Use in combination with Attogene Algae DNA isolation kit Universal 16s PCR primers Perfect for Environmental DNA (eDNA) Characterization
Detail
This kit is sufficient for 150 reactions:
For characterizing cyanobacteria in environmental samples
Use in combination with Attogene Algae DNA isolation kit
Universal 16s PCR primers
Perfect for Environmental DNA (eDNA) Characterization
The 16S ribosomal RNA (rRNA) plays a crucial role in bacterial and archaeal ribosomes. This sequence is Highly conserved across bacteria and archaea, it contains variable regions useful for species differentiation and is part of the 30S subunit of prokaryotic ribosomes. 16s rRNA Binds to the Shine-Dalgarno sequence and contributes to the subunit’s structure. Acts as a scaffold for ribosomal proteins.
Because the 16s rRNA sequence is ubiquitous in bacteria and archaea, it can be used to identify a wide diversity of microbes within a single sample and single workflow.
PEG3-(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane)-(Amino-Tri-(carboxyethoxymethyl)-methane) is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acid groups can react with primary amino groups in the presence of activators (e.g. HATU) to form a stable amide bond.
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PEG3-(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane)-(Amino-Tri-(carboxyethoxymethyl)-methane) is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acid groups can react with primary amino groups in the presence of activators (e.g. HATU) to form a stable amide bond.
DBCO-PEG12-NHS Ester is a monodisperse PEG linker which enable free copper click chemistry with N3 group. NHS ester moiety can react specifically and efficiently with primary amines to form a covalent amide bond. The hydrophilic PEG spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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DBCO-PEG12-NHS Ester is a monodisperse PEG linker which enable free copper click chemistry with N3 group. NHS ester moiety can react specifically and efficiently with primary amines to form a covalent amide bond. The hydrophilic PEG spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
CE-IVD marked version available for in vitro diagnostic use
Vibrio cholerae is a comma-shaped, gram-negative bacterium. It is the cause of cholera in humans, which affects the upper small intestine. Transmission of the disease is mainly through contaminated food or water. Human subjects affected by cholera exhibit severe watery diarrhea and vomiting, caused by the cholera toxin produced by the bacterium. Many cases of cholera are life-threatening, as diarrhea and associated vomiting can lead to rapid dehydration and electrolyte loss. Even with the extensive research on its epidemiology, cholera still affects over 5 million people per year worldwide.
Vibrio cholerae TaqMan PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
Vibrio cholerae TaqMan PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix
Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
