DNaseAlarm – Lateral Flow DNase Activity Assay Kit

Overview

• Rapid isolation of both small and large species of DNA from urine
• Convenient spin column format
• Effective removal of PCR inhibitors
• Purified DNA is highly suited to sensitive downstream applications
• Allows for the purification of viral DNA from urine

Both high molecular weight DNA (greater than 1 kb in size; mostly cell associated) and the smaller DNA (150 – 250 bp; derived from the circulation) is effectively isolated and purified using a rapid and convenient spin column protocol. This kit can be used to isolate DNA from a broad range of viruses in urine as well. Salts, metabolic wastes, proteins and other contaminants are removed to yield inhibitor-free DNA for use in sensitive applications. The DNA is of excellent quality for various downstream applications such as PCR, qPCR and DNA fingerprinting, methylation studies and more.

This kit is fully compatible with Norgen’s Urine Collection and Preservation Tubes.

Urine DNA Isolation Kit

This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 50 μL to 1.75 mL of urine. Multiple samples can be processed in 30 minutes.

Urine DNA Isolation Kit (Slurry Format)

This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 3 mL to 25 mL. Multiple samples can be processed in 30 minutes. Multiple samples can be processed in 45 minutes.

Urine DNA Isolation Maxi Kit (Slurry Format)

This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 25 mL of urine up to 80 mL. Multiple samples can be processed in 45 minutes.

Background

DNA found in urine can be divided into 2 basic categories. The larger species, genomic-DNA (gDNA), is generally greater than 1 kb in size, and appears to be derived mainly from exfoliated cells. The second species is smaller, generally between 150 and 250 bp (apoptotic-DNA), and derives, at least in part, from the circulation. The second species is also considered as an RNA/DNA hybrid as reported by Halicka et al. (2000). Both types of DNA can be isolated reliably using this kit.

Details

Supporting Data

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Kit Specifications – Spin Column
Minimum Urine Input	3 mL
Maximum Urine Input	25 mL
Time to Complete 10 Purifications	< 30 minutes
Size of Urine DNA Purified	Large (> 1 kb) and small (150-250 bp)

Kit Specifications – 96-well
Urine Input Volume	3 – 25 mL
Elution Volume	1st Elution 100 μL 2nd Elution 100 μL
Size of DNA Purified	Large (> 1 kb) and small (150-250 bp)
Time to Complete 96 Purifications	< 1 hour

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm up Slurry B1 and Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed. Slurry B1 contains a grey resin that will not dissolve when warmed.

Component	Cat. 18100 (50 preps)	Cat. 48800 (50 preps)	Cat. 50100 (50 preps)
Binding Solution K	15 mL	–	–
Slurry B1	–	18 mL	18 mL
Proteinase K in Storage Buffer	2 mL	–	–
Pronase K in Storage Buffer	2 mL	–	–
Soluton WN	9 mL	–	–
Binding Buffer A	–	–	50 mL
Lysis Buffer A	–	30 mL	30 mL
Wash Solution A	–	38 mL	38 mL
Wash Solution B	30 mL	–	–
Wash Solution D	9 mL	–	–
Binding Solution K	15 mL	–	–
Elution Buffer B	15 mL	15 mL	15 mL
Micro Spin Columns	50	–	–
Mini Filter Spin Columns	–	50	50
Collection Tubes	50	50	50
Elution Tubes (1.7 mL)	100	100	100
Product Insert	1	1	1

Propargyl-PEG2-alcohol has propargyl and alcohol end groups. The propargyl can participate in copper-catalyzed Click Chemistry reactions with azides. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG2-alcohol has propargyl and alcohol end groups. The propargyl can participate in copper-catalyzed Click Chemistry reactions with azides. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Overview

• Ready-to-use
• Quantitative
• Precise
• Highly Stable
• Thirteen discrete fragments ranging from 300 bp to 10000 bp
• Higher intensity reference band at 5000 bp

The HighRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains thirteen discrete fragments ranging from 300 bp to 10,000 bp with a higher intensity reference band at 5000 bp. This Ladder is ideal for quick sizing of PCR products and restriction digests.

Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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HighRanger 1kb DNA Ladder (Cat# 11900) – 100 loads

Ladder Properties:
• Thirteen discrete bands, ranging from 300 bp to 10000 bp
• Higher intensity band at 5000 bp for easy reference

Fragment	Size (bp)	Mass (ng)
1	10000	58
2	8000	53
3	6000	46
4	5000	67
5	4000	56
6	3000	47
7	2500	41
8	2000	34
9	1500	29
10	1000	18
11	700	13
12	500	23
13	300	14

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 

This kit is stable for 2 years after the date of shipment.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.