DNase activity in a convenient and sensitive lateral flow colormetric assay that delivers results in real time. Great for Quality Testing for DNase contamination of materials and supplies
Detail
DNase activity in a convenient and sensitive lateral flow colormetric assay that delivers results in real time. Great for Quality Testing for DNase contamination of materials and supplies.
Attogene’s DNaseAlarm Lateral Flow test is designed for the sensitive and accurate analysis of DNAse activity in liquid samples. DNase Alarm uses a synthetic DNA substrate that attaches to the streptavidin colloidal reporter molecule (gold) using a 5’ biotin. The DNA substrate also contains a FAM molecule that enables it to be captured by the anti-FAM antibody (test line). In the absence of DNases, the DNA oligo tethers gold to the test line giving a visual test line. When DNases are present, the DNA substrate is degraded, and the gold particles can no longer be tethered to the test line thus, signal is lost. Since the cleavage of the DNA Substrate increases over time when DNase activity is present, results can be evaluated kinetically. This assay has applications for quality control testing and analysis of unit activities of DNase and DNase inhibitors. DNase’s can cause havoc in laboratories working with DNA and are important to perform routine testing.
This test can be used to rapidly and efficiently detect DNase’s in both liquid and on solid surfaces and a perfect tool for monitoring manufacturing.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 6 months under recommended storage conditions
Analyte:
Acetaldehyde
Assay Format:
Spectrophotometer, Microplate, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
0.5 to 20 µg of acetaldehyde per assay
Limit of Detection:
0.18 mg/L
Reaction Time (min):
~ 4 min
Application examples:
Wine, champagne, beer, liqueurs, brandy, dairy products (e.g. yogurt), bread, fruit juices, soft drinks, cocoa, vegetable and fruit products, coffee, and other materials (e.g. biological cultures, samples, etc.).
Method recognition:
Methods based on this principle have been accepted by MEBAK
The Acetaldehyde test kit is a simple, reliable and accurate method for the measurement and analysis of acetaldehyde in beverages and foodstuffs.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Lyophilized format designed for room temperature shipping
Available in TaqMan format for analysis
Norgen’s EBV TaqMan PCR Lyophilized Kit is designed for the detection of EBV specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.
Norgen’s EBV TaqMan Lyophilized Probe/Primer and Control Set is designed for the detection of EBV specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.
All kit components should be stored at -20°C upon arrival.
Once reconstituted, repeated thawing and freezing (>2 times) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
High yield and high quality genomic DNA with no RNA or protein contamination
DNA ready for any application including PCR, qPCR, genotyping and more
Norgen’s Cells and Tissue DNA Isolation Kits are designed for the rapid preparation of genomic DNA from cultured cells as well as various tissue samples and urine. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with PCR and Southern Blot analysis.
Cells and Tissue DNA Isolation Kit (Spin Column)
Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The protocol can be completed in approximately 30 minutes for cells and within 90 minutes for tissues. Each kit contains sufficient materials for 50 preparations.
Cells and Tissue DNA Isolation Micro Kit (Micro)
Optimized for small inputs of cells and tissues, such as Laser-Captured Microdissection (LCM). Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. Preparation time for a single sample is approximately 60 minutes, and each kit contains sufficient materials for 50 preparations.
Cells and Tissue DNA Isolation Kit (Magnetic Bead System)
Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. Preparation for 10 purifications is approximately 40 minutes of hands-on time.
Cells and Tissue DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. The Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) also can be integrated with a robotic automation system.
20 mg of animal tissue Up to 150 μL of viral suspension 3 x 106 cells
Column Binding Capacity
> 50 μg
Average Yield
8 μg (from 1 x 106 HeLa Cells) 10 μg (from 10 mg kidney)
Elution Volume
50 – 200 μL
Analyte Purified
Genomic DNA, mitochondrial DNA, viral DNA
Format
Spin Column
Time to Complete 10 Purifications
30 min (cells) and 90 min (tissue)
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
This test can be used to rapidly and efficiently detect DNase’s in both liquid and on solid surfaces and a perfect tool for monitoring manufacturing. 
