RNase H activity in a convenient and sensitive colormetric assay that delivers results in real time. Great for Quality Testing for RNase H activity in enzyme preparations and determination of RNase H unit activity.
Attogene has developed the first of its kind and patent pending technology that uses a novel RNA/DNA substrate tagged on the 5′ and 3′ end with a Biotin that is attached to our streptavidin colloidal gold reporter molecules and a test line tag. In the absence of RNases H, the gold tagged RNA/DNA molecule will flow up the lateral flow strip and bind to the test line using an anti tag antibody (INDICATING that NO RNase H is detected). When RNases H is present or added, however, the RNA strand of the RNA/DNA substrate is cleaved, and the Biotin tagged 5′ and 3′ tags are spatially separated in solution cauing the test line to disapear.
This test can be used to rapidly and efficiently detect ribonucleases H activity and is a perfect tool for monitoring unit activity or residual RNAse H activity.
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Low Abundance RNA Quantification Kit
Product Info
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Product Info
Overview
Quantify RNA of a wide spectrum of concentrations, including the lower ng per µL and pg per µL range
Compatible with any Real-Time PCR system
RNA is accurately quantified using a standard curve constructed from the provided RNA standard
Norgen’s Low Abundance RNA Quantification Kit offers a PCR-based detection procedure to quantify RNA of a wide spectrum of concentrations, including the lower ng per µL and pg per µL range. The kit has two main enzymatic components – reverse transcription using Norgen’s microScript Reverse Transcription system and PCR Master Mix used in conjunction with a specially formulized primer mixture, to amplify human RNA from different types of inputs (such as various liquid biopsies). The kit is compatible with any Real-Time PCR system with the addition of fluorescent nucleic acid stains such as SYBR Green. The unknown RNA is accurately quantified by using a standard curve constructed from the provided RNA Standard.
Storage Conditions Upon receipt, store Norgen’s Low Abundance RNA Quantification Kit at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.
(1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl Succinimidyl Carbonate is a Succinimidyl ester/NHS-functionalized cyclooctyne derivative for addition of the cyclooctyne moiety into amine-containing compounds or biomolecules. The BCN group can react with azide-tagged biomolecules under copper free conditions.
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(1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl Succinimidyl Carbonate is a Succinimidyl ester/NHS-functionalized cyclooctyne derivative for addition of the cyclooctyne moiety into amine-containing compounds or biomolecules. The BCN group can react with azide-tagged biomolecules under copper free conditions.
Peptone provide carbon and nitrogen sources to meet the needs of bacterial growth; sodium chloride maintains osmotic equilibrium; potassium dihydrogen phosphate and disodium hydrogen phosphate as buffer.
How to use: 1. Suspend 20g of product, adding 1L of distilled or deionized water, heated to boiling stirring until completely dissolved, dispensing into flask, autoclave at 121 ℃ for 15min, set aside. 2.Diluted and treated samples.
Quality control：
Item
The name and number of strain
Growth
Colony Color
1
Salmonella typhi CMCC (B) 50071
good
Cloudy broth
2
Salmonella typhimurium CMCC (B) 50115
good
Cloudy broth
3
Paratyphoid Salmonella CMCC (B) 50093
good
Cloudy broth
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 250g/bottle; 225ml*10bag/box
Reference: 1.GB/T4789.4-2003 People’s Republic of China national standards of food hygiene Examination of Salmonella microorganisms 2.GB/T4789.28-2003 People’s Republic of China national standards of food hygiene microbiological examination Staining, media and reagents 3.SN0170-92 People’s Republic of China Import and Export Commodity Inspection industry standard Salmonella food for export (including Arizona bacteria) test method 4.GB/T4789.4-2008 People’s Republic of China national standards of food hygiene Examination of Salmonella microorganisms 5.GB 4789.4-2010 national standards for food safety standards of food hygiene inspection and microbiological testing of salmonella People’s Republic of China 6.GB 13091-91 People’s Republic of China National Standard Test Method for Salmonella in feed 7.GB 4789.40-2010 national standards for food safety standards of food hygiene inspection microorganism People’s Republic of China E.sakazakii test 8. ISO 6579-2002 Microbiology of food and animal feeding stuffs —– Horizontal method for the detection of Salmonella spp. 9. ISO22964-2006 Milk and milk products —– Detection of Enterobacter sakazakii 10. ISO6785-2001 Milk and milk products —– Detection of Salmonella spp
