DBCO-PEG4-C3-sulfonic acid serves as a bifunctional PEG linker. The DBCO click chemistry handle conjugates with azides on target molecules to form stable triazole linkages. The sulfonic acid can participate in esterification, halogenation and displacement reactions.

DBCO-PEG4-C3-sulfonic acid serves as a bifunctional PEG linker. The DBCO click chemistry handle conjugates with azides on target molecules to form stable triazole linkages. The sulfonic acid can participate in esterification, halogenation and displacement reactions.

For the simultaneous detection of up to four targets in one reaction. Save time, cost, and consumables while maximising data generation.

About

ProbeSure Multiplex Master Mix is an enhanced version of ProbeSure Master Mix, formulated to enable users to analyse up to four targets in one reaction well. For example, two bi-allelic SNPs or one reference gene and a further three genes of interest.

Users will require a plate reader capable of reading FAM, HEX, ATTO 550, ATTO 647N and ATTO 633 (the wavelengths of each of these can be found in our  ProbeSure Multiplex Master Mix User Guide). ProbeSure Multiplex Master Mix is supplied at 2x concentration for convenience and is supplied with the ATTO 633 normalising dye at either high level (500 nM final concentration), low level (25 nM final concentration) or without ATTO 633.

For the simultaneous detection of up to four targets in one reaction. Save time, cost, and consumables while maximising data generation.

Introduction

A highly efficient, easily automated PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. This kit can be easily used in manual and automated 96 or 384-well formats.

Details

Specifications

Features	Specifications
Main Functions	Recover 100-400μl DNA/ RNA from PCR products / enzymatic reaction solution / or crude DNA / RNA
Applications	Automatic sequencing, enzyme digestion, PCR and labeling
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Agarose Gel, PCR products, enzymatic reaction solution
Sample amount	100-400µl
Recovery	80%
Elution volume	≥30μl

Principle

The MagPure method contains magnetic particles in an optimized binding buffer to selectively bind DNA fragments 100bp and larger toparamagnetic beads. Excess primers, nucleotides, salts, and enzymes can be removed using a simple washing procedure.

Advantages

• High recovery efficiency – up to 80% DNA recovery

Kit Contents

Contents	D500301	D500302	D500303
Purification Times	50 Preps	500 Preps	5000 Preps
Buffer AL	10 ml	60 ml	550 ml
Buffer BD*	5 ml	25 ml	2 x 100 ml
MagPure RNA Particles	1.2 ml	12 ml	120 ml
RNase Free Water	5 ml	30 ml	250 ml

Storage and Stability

MagPure RNA Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

A highly efficient, easily automated PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. This kit can be easily used in manual and automated 96 or 384-well formats.