Description
- Appearance: White/Blue with lens
- • Length of strip: 86mm
- • Width of strip: 5mm
- • Length of device: 140mm
- • Width of device: 15mm
HiPure Plant DNA Mini Kit supplies a simple and rapid extraction of genomic DNA from different plant samples. The kit is based on silica gel column and CTAB lysis purification technology. The whole extraction process is only 30-50 minutes. Purified DNA can be used directly for PCR, SSR, AFLP, RAPD and Southern Blot, etc.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from 150 mg plant and fungal tissue |
| Applications | PCR, SSR, AFLP, RAPD and southern blot, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Fresh / frozen plant samples, dried plant / seed samples |
| Sample amount | Fresh / frozen plant samples: 50-150 mgDried plant / seed samples: 15-40 mg |
| Elution volume | ≥40μl |
| Time per run | 30-50 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
This product is based on silica column purification. The sample is lysed with CATB Buffer. DNA is released into the lysate. Cell debris, precipitated proteins and polysaccharides are removed by chloroform extraction. After adjust the binding condition, transfer to an adsorption column. DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Kit Contents
| Contents | D318702 | D318703 |
| Purification Times | 50 Preps | 250 Preps |
| Buffer PAL | 60 ml | 200 ml |
| Buffer GWP | 60 ml | 200 ml |
| Buffer DW1 | 30 ml | 150 ml |
| Buffer GW2* | 20 ml | 50 ml |
| Buffer AE | 20 ml | 60 ml |
| HiPure DNA Mini Columns II | 50 | 250 |
| 2 ml Collection Tubes | 50 | 250 |
Storage and Stability
This product can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
| Name | CAT NO | Fresh / frozen tissue amount | Dried tissue amount | Column type | Elution volume | Yield (muscle) |
| HiPure HP Plant DNA Kit | D3187 | 150mg | 40mg | 1.5ml column | 50 – 100μl | 3-70μg |
| HiPure HP Plant DNA Maxi Kit | D3163 | 5g | 1g | 50ml column | 0.7 – 3ml | 75-1250μg |
| HiPure SF Plant DNA Kit | D3164 | 100mg | 20mg | 1.5ml column | 50 – 100μl | 3-50μg |
| HiPure SF Plant DNA 96 Kit | D3167 | 50mg | 15mg | 96 well plate | 75 – 150μl | 3-30μg |
HiPure Plant DNA Mini Kit supplies a simple and rapid extraction of genomic DNA from different plant samples. The kit is based on silica gel column and CTAB lysis purification technology. The whole extraction process is only 30-50 minutes. Purified DNA can be used directly for PCR, SSR, AFLP, RAPD and Southern Blot, etc.
This kit provides a fast and simple procedure for the effective depletion of major serum proteins including albumin, α-antitrypsin, transferrin and haptoglobin from serum and plasma samples. Such abundant proteins usually obscure less abundant proteins in gel electrophresis making them difficult to visualize and recover. The kit reduces sample complexity to allow for convenient investigation of less abundant proteins. In other applications such as mass spectroscopy, fractionating abundant proteins improves the resolution of less abundant proteins. The kit is ‘generic’ in that it is not antibody-based and can thus be used to deplete abundant proteins from any starting material – human or various other animals – making it more versatile, flexible and affordable than other kits. Resulting proteins are ready for a range of applications including 2D gel electrophoresis, SDS-PAGE, Mass Spectrometry, Protein microarrays, DIGE (Difference Gel Electrophoresis) and more.
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| Kit Specifications | |
| Maximum Column Capacity | 500 μg |
| Minimum Column Capacity | 200 μg |
| Minimum Elution Volume | 30 μL |
| Time to Process 10 Samples | 30 minutes |
Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 17300 (25 preps) |
|---|---|
| Wash Solution G | 125 mL |
| Elution Buffer C | 8 mL |
| Protein Neutralizer | 4 mL |
| Spin Columns | 25 |
| Collection Tubes | 25 |
| Elution Tubes (1.7 mL) | 25 |
| Product Insert | 1 |
The kit is developed for RNA quantification. The RNA Quantification HS kit includes HS Dye, HS Dilution Buffer, and two RNA Standards. Simply dilute the HS Dye with the HS Dilution Buffer, add RNA sample, then read the concentration using the Qubit Fluorometer. The assay is accurate for RNA concentrations from 250 pg/µL to 100 ng/µL (Figure 1). The RNA Quantification High Sensitivity Kit has several advantages over traditional RNA quantitation such as stability, sensitivity, and contaminant tolerance.
Features
The performance of the BioDynami RNA Quantification HS kit is nearly identical to that of Thermo Fisher’s Qubit RNA HS kit (figure below).
Common contaminants such as detergents, solvents, salts, free nucleotides, or protein are well tolerated in the assay (Table 1).
Qubit is a registered trademark of Thermo Fisher Scientific.
The kit is developed for RNA quantification. The RNA Quantification HS kit includes HS Dye, HS Dilution Buffer, and two RNA Standards. Simply dilute the HS Dye with the HS Dilution Buffer, add RNA sample, then read the concentration using the Qubit Fluorometer. The assay is accurate for RNA concentrations from 250 pg/µL to 100 ng/µL (Figure 1). The RNA Quantification High Sensitivity Kit has several advantages over traditional RNA quantitation such as stability, sensitivity, and contaminant tolerance.