Exceptional value for money Rapid detection of all clinically relevant subtypes Positive copy number standard curve for quantification Highly specific detection profile High priming efficiency Broad dynamic detection range (>6 logs) Sensitive to < 100 copies of target
Accurate controls to confirm findings
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
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Malaria TaqMan PCR Detection Kits
Product Info
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Product Info
Overview
Detection kits for Malaria
Available in TaqMan format for analysis
Malaria is a mosquito-borne infectious disease and considered one of the leading causes of death in the world. Malaria is a fatal tropical disease that is caused by a parasite knows as Plasmodium. It is usually spread through the bite of an infected female mosquito. 300-500 million new infected cases are estimated every year with 1.5-2.7 million deaths worldwide. Most of the malaria-related deaths occur in sub-Saharan Africa. . Although blood is the sample most frequently used to make a diagnosis, both saliva and urine have been investigated as alternative, less invasive specimens
Malaria TaqMan PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
Malaria TaqMan PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Usages: For selective isolation of Gram-negative bacteria, especially for Shigella and Salmonella.
Principle: Yeast extract powder provide nitrogen, vitamins, growth factors; sodium chloride to maintain osmotic equilibrium ferric ammonium citrate of iron salts to produce a black iron sulfide; agar as medium coagulant; phenol red as pH indicator.
Formulation（per liter）: Xylose 3.50g L-Lysine 5.00g Lactose 7.50g Sucrose 7.50 g Sodium Chloride 5.00 g Yeast Extract 3.00g Sodium Desoxycholate 2.50g Sodium Thiosulphate 6.80g Fe-Ammonium Citrate 0.80g Phenol Red 0.08g Agar 13.50 Final PH 7.4±0.2
How to use: 1.Suspend 55.2g in 1Lof distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Do not autoclave ,cool to 50℃ and pour into sterile petri dishes. 2.Dilluted and treated samples.
Quality control: Quality control strains were inoculated ,and cultured at 36 ± 1 ℃ for 24h ,results show as follows: strain name strain code growth feature Arizona bacteria CMCC (B) 47001 good black colonies Salmonella typhimurium CMCC (B) 50115 good black colonies Streptococcus faecalis CMCC32223 prohibited —
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
