Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Other Products
TD4Z Mini Centrifuge – Vortex
Product Info
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Product Info
Product Highlights
Speed: Adjustable speed, up to 4500 rpm
Vortex Oscillation Intensity:1-4 intensity levels, selectable and adjustable
Programming:Programmable,freely choose combinations of oscillation strength,duration.centrifugation speed, and time
Safety:Safety interlock device to ensure user safety
Compatibility: Single tube or multi-tube centrifugation/mixing with one-button operation, saving time and cost
Display:Touch buttons and an LCD screen clearly indicate and set oscillation level, duration, and cycle count
Convenience: Multi-functional, compact design, occupies minimal space
Technical Specifications
Dimensions (mm)
200(w)x243(H)x127(D)
Working temperature
5℃ to 40℃
Oscillation Duration
0-30 seconds (increment of 1 second)
TD4Z Speed range
1000-3600 rpm (increment of 100 rpm)
Power Supply
AC 220V 50/60 Hz
Spin Timer
0 seconds to 30 minutes
Weight
3.5kg (including power supply)
Oscillation Intensity
Level 1-4
Speed range TD4Z
1-99 programs
Centrifugation Adjustment TD4Z
1000-3600 rpm (increment of 100 rpm)
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PCR laboratories and general molecular biology laboratories
Model:
TD4Z
Solid Phase Reversible Immobilization (SPRI) beads are a simple and effective reagent for DNA purification, but not for short oligo purification. The beads are paramagnetic particles coated with carboxyl groups that can reversibly bind to nucleic acid. However, SPRI beads can only purify DNA/RNA fragments that are 100 base pairs or longer. DNA/RNA fragments shorter than 100 base pairs are not effectively recovered.
Oligo purification can also be performed using spin column-based technology. The oligo size limitation for recovery is around 20 nt, as oligos under 20 nt have a very low recovery rate.
We have developed Magnetic Beads (Short Oligo Purification) Kit for short oligo purification. Our proprietary bead technology enables the recovery of oligos as short as 6 nt. 80% of the 6 nt oligos and 90% of the > 8 nt oligos can be recovered. The reagent also effectively removes impurities and unwanted components such as salts, proteins, dNTPs, detergents, and other contaminants. The magnetic bead reagents are RNase free, and can be used for both DNA and RNA applications.
Recovery rates of of short oligos. Oligos with different sizes were used as input. BioDynami Short Oligo Quantification Kit (Cat. # 40046) was used to quantify the recovery rates with modifications.
Features
Effective purification of short oligos
6 nt oligos: 80% recovery rate
>8 nt oligos: >90% recovery rate
Removal of impurities and unwanted reaction components
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Solid Phase Reversible Immobilization (SPRI) beads are a simple and effective reagent for DNA purification, but not for short oligo purification. The beads are paramagnetic particles coated with carboxyl groups that can reversibly bind to nucleic acid. However, SPRI beads can only purify DNA/RNA fragments that are 100 base pairs or longer. DNA/RNA fragments shorter than 100 base pairs are not effectively recovered.
Attogene’ s Cylindrospermopsin Lateral Flow Kit can be used to detect Cylindrospermopsin in source water samples.
Format: Rapid-Water – Run Time: 30 Minutes, enough to run two samples at 10 and 100 fold dilutions and two negative controls.
Cylindrospermopsin (CYN) is a potent cyanotoxin synthesized by select species of cyanobacteria, prominently including Cylindrospermopsin raciborskii. It belongs to the tricyclic alkaloid class, exhibiting a molecular weight of approximately 415 Da. Structurally, cylindrospermopsin features an uracil ring fused with a hydantoin moiety, alongside a guanidino group, attributes that render it highly soluble and polar in aqueous environments.
Cylindrospermopsin is notorious for its profound toxicity towards aquatic organisms and its potential threat to human health through exposure via contaminated water and food sources. Consequently, rigorous monitoring protocols are essential in regions prone to cyanobacterial blooms, where cylindrospermopsin can accumulate in freshwater reservoirs and other aquatic habitats. In recognition of these risks, regulatory bodies such as the United States Environmental Protection Agency (EPA) have implemented an action level guideline. As of 2019, EPA 10-day drinking water health advisory for cylindrospermopsin recommended a threshold of 0.7 parts per billion (ppb), or 700 parts per trillion (ppt) for children under the age of six, and 3 parts per billion, 3000 parts per trillion for anyone older, to effectively manage cylindrospermopsin levels. This precautionary measure aims to uphold both environmental sustainability and public health integrity by minimizing exposure risks. The EPA has also drafted a human health recreational water quality criterion to protect human health at 8,000ppt.
Do not Drink – 0.7 μg/L for bottle fed infants and preschool children, pregnant and nursing woman, elderly immunocompromised and liver conditions
Do not Drink – 3.0 μg/L for school age children to adults
Do Not Use – 20 μg/L
EPA Draft Human Health Recreational Ambient Water Quality Criteria to protect human health: 8 μg/L
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Screening of Cylindrospermopsin in water samples at or above 30ppt
Format: 15 tests (5 samples at 2 dilutions/5 controls)
Syringe
Sample Filter
Sample Dilution Buffer
Water Collection Bottle
Water Collection Tube
200ul fixed volume pipette
Water Sample Bottle
Negative control
