Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Other Products
Urine Collection and Preservation Devices
Product Info
Document
Product Info
Overview
RNA/microRNA/DNA/Proteins are preserved for more than 2 years at room temperature in Norgen’s Urine Preservative
Compatible with most DNA, Total RNA, microRNA and protein isolation methods
Preservative is available in a single dose liquid format (ampule)
Preservative is also available in a dried format in tubes – Urine Collection and Preservation Tubes
Urine Collection and Preservation Devices are available in 4 convenient sizes: 5 cc tubes, 15 cc tubes, 50 cc tubes, and 120 cc cups
Norgen’s Urine Preservative is designed for the rapid preservation of DNA, RNA, microRNA and proteins from fresh urine samples. In addition, the Urine Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins.
Norgen’s Urine Preservative is available in 2 convenient formats:
1. Urine Preservative Single Dose Ampules With this product Norgen’s Urine Preservative is supplied in a liquid format in single dose ampules. The user simply collects 5 – 50 mL of urine into a urine collection container and adds the contents of the Urine Preservative Single Dose (Cat# 18126). The urine and preservative are then mixed, and the urine nucleic acids and proteins are preserved at room temperature.
2. Urine Collection and Preservation Tubes Norgen’s Urine Preservative is also available in a dried format in Norgen’s Urine Collection and Preservation Tubes. The user simply collects urine into the tubes and mixes gently until the orange preservative pellet in the tube has dissolved. Norgen’s Urine Collection and Preservation Tubes are available in 3 convenient sizes:
Select Urine Tested with the Norgen Urine Collection and Preservation Tubes
Human Mouse Lynx Wolf Urine collected from snow
Available Sizes and Formats
Urine Collection and Preservation Tubes (50 cc) – Urine inputs from 5 – 45 mL
Storage Conditions and Product Stability All tubes should be kept tightly sealed and stored at room temperature (15 – 25°C) for up to 2 years without any reduction in performance.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
L-Arabinose, D-Galactose
Assay Format:
Spectrophotometer, Microplate, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
4 to 80 μg of L-arabinose or D-galactose per assay
Limit of Detection:
0.58 mg/L (L-arabinose), 0.69 mg/L (D-galactose)
Reaction Time (min):
~ 12 min (L-arabinose), ~ 6 min (D-galactose)
Application examples:
Analysis of hydrolysates of oligo- and polysaccharides (e.g. arabinan, arabinoxylan, galactan, arabinogalactan), milk, dairy products, foods containing milk (e.g. dietetic foods, bakery products, baby food, chocolate, sweets and ice-cream), food additives (e.g. sweeteners), cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.).
Method recognition:
Novel method
The L-Arabinose/D-Galactose test kit is a simple, reliable and accurate UV method for the measurement and analysis of L-arabinose and/or D-galactose in various materials including foods, feeds, beverages and plant products.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
Very rapid reaction due to inclusion of galactose mutarotase (patented technology)
Very cost effective
All reagents stable for > 2 years after preparation
Only enzymatic kit available
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Suitable for manual, microplate and auto-analyser formats
Document
The L-Arabinose/D-Galactose test kit is a simple, reliable and accurate UV method for the measurement and analysis of L-arabinose and/or D-galactose in various materials including foods, feeds, beverages and plant products.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
endo-Cellulase
Assay Format:
Spectrophotometer, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
400
Signal Response:
Increase
Limit of Detection:
1.2 x 10-3 U/mL
Reproducibility (%):
~ 3%
Total Assay Time:
10 min
Application examples:
Fermentation broths, industrial enzyme preparations and biofuels research.
Method recognition:
Novel method
The K-CellG5-2V pack size has been discontinued (read more).
Cellulase Activity Assay Kit.
The CellG5 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) and 2) thermostable β-glucosidase. The ketone blocking group prevents any hydrolytic action by the β-glucosidase on BPNPG5. Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of BPNPG5 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).
The CellG5 assay represents a huge step forward in the methodology for the measurement of cellulase that traditionally relied on substrates such as CM-cellulose, Avicel, cellooligosaccharides, filter paper or dyed polysaccharides including CMC Congo red or cellulose azure.
The CellG5 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) and 2) thermostable β-glucosidase. The ketone blocking group prevents any hydrolytic action by the β-glucosidase on BPNPG5. Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of BPNPG5 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).
