Naegleria Fowleri

Introduction

Usages:

For confirmatory test of colifrom in water.

Principle:

Peptone, yeast extract powder, beef extract powder provides carbon and nitrogen sources, vitamins and minerals; lactose as fermentable sugars; agar as medium coagulant; potassium hydrogen phosphate as buffer.

Formulation(per liter):

Peptone 10g

Yeast extract powder 5g

Beef extract powder 5g

Lactose 10g

Agar 15g

Dipotassium hydrogen phosphate 3.5g

Sodium sulphite 5g

Fuchsin 1g

Final pH 7.2 ± 0.2

How to use:

1.Suspend 54.5g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, autoclave at 115 for 20min. Cool to 50 and pour into petri dishes.

2.Diluted and treated samples.

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

500g

Overview

• Reduce endotoxin levels to 0.1 EU/µg DNA or less
• Available in 3 formats: Mini, Midi, and Maxi to suit your desired input volume
• Remove endotoxins from up to 1 mg of DNA with the Maxi format kit
• Fast and easy processing using a rapid spin-column format

These kits are designed for the rapid spin column removal of endotoxins from previously purified DNA. Norgen’s spin columns bind DNA while endotoxins, salts and other contaminants are washed away. These kits reduce endotoxins to 0.1 EU/μg DNA or less providing plasmid DNA that is immediately ready for transfections or other endotoxin-sensitive applications. Typical recovery of DNA is >90% of the starting sample.

Endotoxin Removal Kit (Mini)

This kit is designed for the rapid spin column removal of endotoxins from up to 25 µg of previously purified DNA. The convenient spin column procedure can be completed in approximately 20 minutes.

Endotoxin Removal Kit (Midi)

This kit is designed for the rapid spin column removal of endotoxins from up to 200 μg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.

Endotoxin Removal Kit (Maxi)

This kit is designed for the rapid spin column removal of endotoxins from up to 1 mg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.

About Endotoxins

Endotoxins (also called lipopolysaccharides), are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin sensitive cell lines. Therefore, the removal of endotoxins from plasmid preparations is often necessary prior to the use of the DNA in such downstream applications.

Details

Supporting Data

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Kit Specifications
Maximum DNA Input	25 μg
Final Endotoxin Level	≤ 0.1 EU/µg DNA
Size of DNA Purified	Up to 13,000 bp
Maximum DNA Volume Input	100 μL
Average Recovery	> 90%
Time to Complete 10 Purifications	20 minutes

 
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 22700 (25 preps)	Cat. 52200 (10 preps)	Cat. 21900 (4 preps)
Buffer SK	15 mL	60 mL	60 mL
Wash Solution H	18 mL	18 mL	18 mL
Elution Buffer I	6 mL	12 mL	12 mL
Endotoxin Removal Solution	1.5 mL	1.5 mL	1.5 mL
Precipitation Solution	–	1.5 mL	1.5 mL
Spin Columns (assembled with Collection Tubes)	–	10	4
Spin Columns	25	–	–
Collection Tubes	25	–	–
Elution Tubes (1.7 mL)	25	–	–
Elution Tubes (15 mL)	–	10	–
Elution Tubes (50 mL)	–	–	4
Product Insert	1	1	1

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target Accurate controls to confirm findings