Exceptional value for money Rapid detection of all clinically relevant subtypes Positive copy number standard curve for quantification Highly specific detection profile High priming efficiency Broad dynamic detection range (>6 logs) Sensitive to < 100 copies of target
Accurate controls to confirm findings
50 & 150 reactions
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection
Product parameters:
Kit composition(WLRE8208KIT 48T/Pack)
Composition
Content
E buffer
1 mL×2 Tubes
B buffer
150 μL×1Tube
Positive control template
100 μL×1Tube
Reagents
48T
Product features and advantages:
Product features and advantages
Features
Advantages
High sensitivity
1 copies/ml
Strong specificity
Specificity is superior to PCR
Short reaction time
20min
Polymorphic reagent
Liquid, freeze-dried powder, freeze-dried ball
Easy to operate
Few steps of liquid dispensing; It is even possible to add only samples and amplify to get the resultsDesign of primers and probe is simple
Easy to store and transport
It is best preserved at -20℃, and can be stored at room temperature for up to 30 days in a cool place away from light.The freeze-dried form has a long storage time and is convenient for transportation
Low requirements on equipment
Applicable to Applied Biosystems 7500, QuantStudio 3/5, QuantStudio 6/7 Flex fluorescence quantitative PCR instrument;Applicable to our WL-16-III and other isothermal fluorescence detector
Product application:
Application
Ultra-clean laboratory
African Swine Fever Virus Isothermal Detection
Indoor
Operation procedure and process:
Operation procedure and process:
Take out the liquid components of the kit in advance, thaw at room temperature, and shake to mix.
Prepare freeze-dried reagents according to the number of samples to be tested, negative and positive controls, and add 37.5 μL E buffer to each freeze-dried reagent.
Select the appropriate nucleic acid extraction method and reagent to extract the sample nucleic acid
Add 10 μL nucleic acid template to the reaction tube (the amount of template can be adjusted to be filled with sterile water; That is, 10 μL nucleic acid template plus sterile water), 10 μL positive control template was added to positive control, and 10 μL sterile water was added to negative control
Add 2.5μL B buffer to each reaction tube and close the tube cap (for multiple reactions, it is recommended to add B buffer to the inside of the tube cap)
Thoroughly mix the reaction tube upside and down for 8-10 times. After mixing, shake (or rapidly centrifuge) the reaction liquid to the bottom of the tube and transfer it to the amplification zone.
Performence Comparison Data:
No.
TT value
Template concentration
Result determination
1
4.2
10-1
Positive
2
5.5
10-2
Positive
3
10.2
10-3
Positive
4
17.0
10-4
Positive
5
12.0
10-5
Positive
6
–
10-6
Negative
7
–
10-7
Negative
8
Negative control
Negative
Support and Services:
Livestock Disease Kit Technical Support and Services
We provide technical support and services for our Livestock Disease Kit. We are committed to helping you get the most out of your product and ensuring your satisfaction with our products and services.
Our technical support staff is available to answer any questions you may have about the product.
We offer online tutorials and user guides to help you understand the product better.
We provide technical assistance to help troubleshoot any issues you may be having with the product.
We offer a warranty on our products.
If you have any questions or need technical support, please do not hesitate to contact us. We are here to help you make the most of your Livestock Disease Kit.
FAQ:
Q: What diseases can Livestock Disease Kit detect? A: Livestock Disease Kit can detect different infectious diseases, such as Foot and Mouth Disease, Bluetongue Disease, Bovine Viral Diarrhea.
Q: How does Livestock Disease Kit work? A: Livestock Disease Kit uses advanced MIRA technology to amplify the target DNA/RNA targent sequences , and detect the disease by analyzing the amplified sequences.
Q: How long does it take to get results from Livestock Disease Kit? A: Livestock Disease Kit can give a result within 5-20 mins.
Q: What type of sample is suitable for Livestock Disease Kit? A: Livestock Disease Kit is suitable for blood, serum, and tissue samples.
Q: Does Livestock Disease Kit require professional equipment? A: No, Livestock Disease Kit does not require any professional equipment,can use with our isothermal equipment or pcr machine
Document
Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection
CE-IVD marked version available for in vitro diagnostic use
Available in TaqMan format for analysis
Norovirus is a single-stranded, non-enveloped RNA virus belonging to the Caliciviridae family. Norovirus is considered the major causative agent of non-bacterial gastroenteritis in the world. The main channel of transmittance is via contaminated food or water, as well as human-to-human contact. Most Noroviruses infecting humans belong to genogroup I and II (particularly genotype 4). Norovirus is very stable in the environment and is resistant to some surface disinfectants such as alcohols and detergents. Moreover, it is very difficult to culture Norovirus in vitro, making its identification by standard microbiological assays challenging.
NoV TaqMan RT-PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
NoV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix
Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
