Enterovirus Species

Overview

• Detection kits for Candida albicans
• Lyophilized format designed for room temperature shipping
• Available in TaqMan format for analysis

Norgen’s Candida albicans TaqMan PCR Lyophilized Kit is designed for the detection of Candida albicans specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.

Norgen’s Candida albicans TaqMan Lyophilized Probe/Primer and Control Set is designed for the detection of Candida albicans specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.

Details

Supporting Data

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Storage Conditions and Product Stability

• All kit components should be stored at -20°C upon arrival.
• Once reconstituted, repeated thawing and freezing (>2 times) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
• Each kit is provided with 4 tubes of 2X PCR Master Mix and each tube is enough to run 25 reactions. It is not necessary to reconstitute all Master Mix tubes at once. The Master Mix tubes can be reconstituted as and when needed.
• All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

Component	Cat. TM34050L (100 preps)	Cat. TM34010L (100 preps)
MDx TaqMan 2X PCR Master Mix (Lyo)	4 x 350 μL	–
Candida albicans Primer & Probe Mix (Lyo)	280 μL	280 μL
Candida albicans Positive Control (Lyo)	150 μL	150 μL
Nuclease-Free Water (Negative Control)	3 x 1.25 mL	1 x 1.25 mL
Product Insert	1	1

Description 

The PM5000 ExcelBand™ 3-color Pre-Stained Protein Ladder Regular Range is a ready-to-use three-color protein standard with 13 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine Buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and 10 to 170 kDa Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The PM5000 ExcelBand™ 3-color Pre-Stained Protein Ladder Regular Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Features

• Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.
• Three reference bands — 75 kDa (red), 40 kDa (green), and 20 kDa (blue)

Contents 

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol). 

Quality Control 

Under suggested conditions, PM5000 ExcelBand™ 3-color Pre-Stained Protein Ladder Regular Range resolves 13 major bands in SDS-PAGE (Tris-Glycine, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane. 

Storage

4°C for 3 months 
-20°C for long term storage

The PM5000 ExcelBand™ 3-color Pre-Stained Protein Ladder Regular Range is a ready-to-use three-color protein standard with 13 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine Buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and 10 to 170 kDa Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The PM5000 ExcelBand™ 3-color Pre-Stained Protein Ladder Regular Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Overview

• High yield and high purity DNA ready for any application
• Available in a variety of formats to properly suit your needs
• Compatible with blood collected on a variety of commercially available tubes

These kits allow for the isolation of DNA from the blood of various species, including humans and will recover genomic DNA, mitochondrial DNA, viral DNA and bacterial DNA. The purified DNA is of excellent quality and yield and completely compatible with any downstream application including PCR, qPCR and genotyping.

Blood DNA Isolation Mini Kit

Norgen’s Blood DNA Isolation Mini Kit is designed for the rapid preparation of DNA from up to 200 µL of whole blood using a rapid spin column protocol. Preparation time for a single sample is less than 30 minutes and each kit contains sufficient materials for 50 preparations.

Blood DNA Isolation Midi Kit

Norgen’s Blood DNA Isolation Kit is designed for the rapid spin column preparation of DNA from 0.3 to 2 mL volumes of whole blood. Preparation time for a single sample is less than 30 minutes, and each kit contains sufficient materials for 20 preparations.

Blood DNA Isolation Maxi Kit

This kit is designed for the rapid preparation of DNA from 3 mL up to 10 mL of whole blood. Preparation time for a single sample is less than 30 minutes.

Blood DNA Isolation 96-Well Kit (High Throughput)

This kit provides a rapid method for the high-throughput isolation of DNA from up to 200 µL of whole blood. Fast and easy processing using either a vacuum manifold or centrifugation.

Blood DNA Isolation Kit (Magnetic Bead System)

Norgen’s Blood DNA Isolation Kit (Magnetic Bead System) is designed for the rapid preparation of genomic DNA from up to 200 µL of whole blood from various species, including human. Purification is based on magnetic beads as the separation matrix. Norgen’s magnetic beads bind DNA under optimized salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The genomic DNA is preferentially purified from other cellular proteinaceous components. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including real-time PCR, NGS and microarray analysis.

Blood DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)

96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.

Details

Supporting Data

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Kit Specifications
Maximum Blood Input	200 µL
Average Yield (200 µL blood)*	4-12 µg
Average Purity (OD260/280)	1.8 – 1.9
Time to Complete Purifications	40 minutes (hands-on time Cat. 59800) 50 minutes (hands-on time Cat. 62600)

* Average DNA yield will vary depending on the donor

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Component	Cat. 46300 (50 preps)	Cat. 46380 (100 preps)	Cat. 51400 (20 preps)	Cat. 31200 (12 preps)	Cat. 46350 (192 preps)	Cat. 59800 (50 preps)	Cat. 62600 (192 preps)
Lysis Buffer B	20 mL	2 x 20 mL	2 x 40 mL	2 x 110 mL	2 x 40 mL	20 mL	1 x 40 mL 1 x 20 mL
Solution WN	18 mL	2 x 18 mL	55 mL	55 mL	55 mL	18 mL	55 mL
Wash Solution A	18 mL	2 x 18 mL	2 x 38 mL	2 x 38 mL	2 x 38 mL	–	–
Elution Buffer B	30 mL	2 x 30 mL	30 mL	30 mL	2 x 30 mL	15 mL	1 x 30 mL 1 x 15 mL
Proteinase K in Storage Buffer	1.2 mL	2 x 1.2 mL	4.4 mL	8 mL	4.5 mL	1.2 mL	4 mL
Magnetic Bead Suspension	–	–	–	–	–	2 x 1.1 mL	8.5 mL
Maxi Spin Columns	–	–	–	12	–	–	–
Mini Spin Columns	50	100	–	–	–	–	–
Midi Spin Columns	–	–	20	–	–	–	–
96-Well Plate	–	–	–	–	2	–	2
Adhesive Tape	–	–	–	–	4	–	2
Collection Tubes	50	100	20	12	–	–	–
96-Well Collection Plate	–	–	–	–	2	–	–
Elution Tubes	50	100	20	12	–	50	–
96-Well Elution Plate	–	–	–	–	2	–	2
Product Insert	1	1	1	1	1	1	1