Attogene’s Carbon Dioxide Enzymatic Assay Kit is a simple, direct method for measuring Carbon Dioxide levels in the environment. The assay uses a coupled enzyme assay to detect CO2 (as HCO3-) as follows. In the first step, the bicarbonate condenses with phosphoenol pyruvate to form oxalate (and phosphoric acid); this reaction is catalyzed by the enzyme Phosphoenolpyruvate Decarboxylase, PEPC. The oxalate is then enzymatically reduced by the enzyme Malate Dehydrogenase (using an NADH cofactor) to form malate and NAD+.
Amp-Future Isothermal Amplification Kit, RNA Detaction, Freeze-Dried Form, High Specificity
Product Detail
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature:at room temperature and constant temperature(generally 39℃~42℃),reverse transcriptaseuses specific primers and template RNA tosynthesize cDNA strands,and binds the auxiliary protein and single strand with the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology positionand strand exchange begins; accompanied by the recombinase from the complex upon dissociation,the polymerase also bindsto the 3′ end of the primer,initiating chainextension.It is suitable for laboratory-level RNA amplification and RNA amplification for other detection purposes.
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
Parameters
Details
Product Name
RNA Isothermal Amplification Kit Basic
Manufacturer
Amp-future
Storage Temperature
-20°C
Kit Components
Enzymes, Buffers ,Reagents
Packaging
48 Tests/box
Detection Limit
500-1000copies/µL
Shipping
ICE
Test Time
5-20mins
Isothermal nucleic acid Applications
Suitable for RNA isothermal rapid amplification kit(Basic type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
RNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
Fast and easy high throughput processing using either a vacuum manifold or centrifugation
Process all soil types including clay, loam, sandy soils and high humic content soils such as peat, compost and manure
Remove organic substances using the OSR Solution
Remove all humic acid from DNA samples using the Humic Acid Removal plate
Isolate high quality total DNA from all soil types – ready for any downstream PCR, qPCR
Excellent DNA for metagenomic studies
No phenol or chloroform extractions
This kit provides a fast, reliable and simple procedure for high throughput isolation of DNA from all types of soil samples including common soil samples and difficult soil samples with high humic acid content such as compost and manure. A combination of chemical and physical homogenization effectively lyses all microorganisms in the soil sample, and the kit removes all traces of humic acid using the provided Organic Substance Removal (OSR) Solution and Humic Acid Removal plate (HAR). Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR and qPCR applications and more for any metagenomic study, as all humic acid substances and PCR inhibitors are removed during the isolation.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
DBCO-NHCO-PEG4-acid is an analog of DBCO-Acid with a hydrophilic PEG spacer arm, which improves water solubility. This reagent is a non-activated building block with enhanced solubility in aqueous media and can thus be used to derivatize amine-containing molecule through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
DBCO-NHCO-PEG4-acid is an analog of DBCO-Acid with a hydrophilic PEG spacer arm, which improves water solubility. This reagent is a non-activated building block with enhanced solubility in aqueous media and can thus be used to derivatize amine-containing molecule through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
