Description
Specifications:
| Clone | IHC512 |
| Source | Mouse Monoclonal |
| Positive Control | Hairy Cell Leukemia |
| Dilution Range | 1:200 |
Annexin A1 (ANXA1) is a membrane protein that plays a role in innate and adaptive immunity by controlling the biosynthesis of inflammation, prostaglandins, and leukotriene mediators. This target is overexpressed in 97% of all samples from patients with with hairy cell leukemia, and is absent in other B-cell lymphomas. High ANXA1 expression is frequently associated with advanced stage esophageal and esophagogastric junction adenocarcinoma, and is also linked to advanced and metastatic disease states.
| Clone | IHC512 |
| Source | Mouse Monoclonal |
| Positive Control | Hairy Cell Leukemia |
| Dilution Range | 1:200 |
MagPure Stool DNA Kit is specially designed for high throughput DNA extraction from stool samples. It can get high purity microbial DNA from stool samples (≤200mg). This kit is based on magnetic beads purification and unique inhibiting factors adsorption technology, no phenol-chloroform extraction or alcohol precipitation. It can adsorb humic acid and other inhibiting factors in the solution efficiently. DNA can be directly used for downstream applications such as PCR, Viral DNA testing, bacterial DNA testing, etc.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from 100-150mg stool samples |
| Applications | PCR, southern blot and enzyme digestion, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Stool |
| Sample amount | 100-150 mg |
| Elution volume | |
| Time per run |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Kit Contents
| Contents | D636401 | D636402 | D636404 |
| Purification Times | 48 Preps | 96 Preps | 400 Preps |
| MagPure Particles N | 1.7 ml | 3.4 ml | 14 ml |
| 2ml Bead Tubes | 48 | 96 | 400 |
| RNase A | 10 mg | 20 mg | 75 mg |
| Proteinase K | 24 mg | 48 mg | 180 mg |
| Protease Dissolve Buffer | 3 ml | 5 ml | 20 ml |
| Buffer ATL | 60 ml | 110 ml | 300 ml |
| PVP-10 | 1.2 g | 2.2 g | 6 g |
| Buffer PCI | 50 ml | 100 ml | 300 ml |
| Buffer MLE | 30 ml | 60 ml | 180 ml |
| Buffer GW1* | 22 ml | 44 ml | 132 ml |
| Elution Buffer | 20 ml | 20 ml | 60 ml |
Storage and Stability
MagPure Particles, RNase A and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
MagPure Stool DNA Kit is specially designed for high throughput DNA extraction from stool samples. It can get high purity microbial DNA from stool samples (≤200mg). This kit is based on magnetic beads purification and unique inhibiting factors adsorption technology, no phenol-chloroform extraction or alcohol precipitation. It can adsorb humic acid and other inhibiting factors in the solution efficiently. DNA can be directly used for downstream applications such as PCR, Viral DNA testing, bacterial DNA testing, etc.
100 bp DNA Ladder in 2% of agarose gel.
• For sizing and quantification of double strand DNA fragments.
• Composed of nine bands as shown on right.
• The 500-bp band with higher concentration is easily distinguishable from the others.
• Premixed with 6X DNA loading buffer for direct gel loading.
100 bp DNA Ladder in 2% of agarose gel.
• For sizing and quantification of double strand DNA fragments.
• Composed of nine bands as shown on right.
• The 500-bp band with higher concentration is easily distinguishable from the others.
• Premixed with 6X DNA loading buffer for direct gel loading.
Norgen’s EXTRAClean Urine Cell-Free Circulating RNA Purification Kits provide a fast, reliable, reproducible, and simple procedure for isolating circulating RNA and exosomal RNA from various urine inputs ranging from 250 μL and up to 30 mL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extensions, and expression array assays.
EXTRAClean Cell-Free Circulating RNA Purification Mini Kit:
For sample volumes ranging from 250 μL to 2 mL
EXTRAClean Cell-Free Circulating RNA Purification Midi Kit:
For sample volumes ranging from 2mL to 10mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL
EXTRAClean Cell-Free Circulating RNA Purification Maxi Kit:
For sample volumes ranging from 10 mL to 30 mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL.
Figure 1 / 3
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| Kit Specifications | |
|---|---|
| Sample Type | Fresh or frozen urine |
| Sample Volume Range | 2 to 10 μL |
| Minimum Elution Volume | 50 μL |
| Maximum Elution Volume | 100 μL |
| Time to Complete 10 Purifications | 40–45 minutes |
| Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
| Average Yields ¥ | Variable depending on specimen |
¥Please check page 6 of the protocol for average urine yields and common RNA quantification methods.
All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
| Component | Mini (Cat. 72900) | Midi (Cat. 72910) | Maxi (Cat. 72920) |
|---|---|---|---|
| Binding Solution K | 25 mL | 75 mL | 1 x 75 mL 1 x 25 mL |
| Lysis Buffer A | 30 mL | 20 mL | 20 mL |
| Wash Solution A | 18 mL | 18 mL | 18 mL |
| Elution Solution A | 6 mL | 6 mL | 6 mL |
| Collection Tubes | 50 | 20 | 10 |
| Elution Tubes (1.7 mL) | 50 | 20 | 10 |
| EXTRAClean Mini Spin Columns | 50 | 20 | 10 |
| EXTRAClean Midi Spin Columns | – | 20 | – |
| EXTRAClean Maxi Spin Columns | – | – | 10 |
| Product Insert | 1 | 1 | 1 |
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Email : hej@a3p-scientific.com
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