Introduction

Usages:

 For cultivating candida albicans.

Principle:

Corn flour extract provide carbon and other nutrients; polysorbate 80 as neutralizer; agar as medium coagulant.

Formulation(per liter):

Corn extract 6g

Polysorbate 80   10ml

Agar 15g

Final pH 5.6 ± 0.2

How to use:

1.Suspend 21g and 10ml of Tween 80 in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121 for 15 minutes.

2.Diluted and treated samples.

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

500g

Introduction

HiPure Bacterial RNA Kit uses silica gel column purification to simplify the extraction. The whole process does not require phenol chloroform extraction and time-consuming alcohol precipitation. The kit is suitable for efficiently extracting RNA from various bacterial samples. The purified RNA can be directly used for RT-PCR, Northern hybridization, etc. The kit has included lysozyme and glass beads, which can be used to treat gram-negative bacteria which is easy to be lysed, as well as gram-positive bacteria which is hard to be lysed, including enterococcus faecalis, staphylococcus, etc.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA from bacteria culture
Applications	RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Bacteria culture
Sample amount	Bacteria: <10^9
Elution volume	≥30μl
Time per run	≤40 minutes
Liquid carrying volume per column	800µl
Binding yield of column	100µg

Principle

Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

Advantages

• Fast – several samples can be extracted in 30 minutes
• High purity – the purified RNA can be directly used in various downstream applications
• High recovery – RNA can be recovered at the level of PG
• Good repeatability – silica gel column purification technology can obtain ideal results every time
• Broad spectrum – it can deal with various bacteria, including Gram-positive bacteria that are difficult to be lysed
• Sufficient components – the kit contains carried lysozyme and glass beads

Kit Contents

Contents	R418101	R418102	R418103
Purification Times	10 Preps	50 Preps	250 Preps
gDNA Filter Mini Columns	10	50	250
HiPure RNA Mini Columns	10	50	250
2ml Collection Tubes	20	100	500
Glass Beads (0.1-0.6mm)	10 g	30 g	150 g
Plastic spoon	2	4	10
Lysozyme	20 mg	90 mg	400 mg
Protease Dissolve Buffer	1.8 ml	1.8 ml	10 ml
Buffer TE	1.8 ml	1.8 ml	5 ml
Buffer STL	5 ml	20 ml	90 ml
Buffer RLC	10 ml	30 ml	150 ml
Buffer RW1	10 ml	50 ml	250 ml
Buffer RW2*	5 ml	20 ml	2 x 50 ml
RNase Free Water	1.8 ml	10 ml	30 ml

Storage and Stability

The kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions. Due to the lack of antibacterial agents, RNase Free Water may be contaminated by bacterial or fungal when placed or operated at room temperature. It is recommended to pack and store at 2-8°C to reduce contamination.

HiPure Bacterial RNA Kit uses silica gel column purification to simplify the extraction. The whole process does not require phenol chloroform extraction and time-consuming alcohol precipitation. The kit is suitable for efficiently extracting RNA from various bacterial samples. The purified RNA can be directly used for RT-PCR, Northern hybridization, etc. The kit has included lysozyme and glass beads, which can be used to treat gram-negative bacteria which is easy to be lysed, as well as gram-positive bacteria which is hard to be lysed, including enterococcus faecalis, staphylococcus, etc.

Overview

• Fast and easy high throughput processing using either a vacuum manifold or centrifugation
• Process all soil types including clay, loam, sandy soils and high humic content soils such as peat, compost and manure
• Remove organic substances using the OSR Solution
• Remove all humic acid from DNA samples using the Humic Acid Removal plate
• Isolate high quality total DNA from all soil types – ready for any downstream PCR, qPCR
• ​Excellent DNA for metagenomic studies
• No phenol or chloroform extractions

This kit provides a fast, reliable and simple procedure for high throughput isolation of DNA from all types of soil samples including common soil samples and difficult soil samples with high humic acid content such as compost and manure. A combination of chemical and physical homogenization effectively lyses all microorganisms in the soil sample, and the kit removes all traces of humic acid using the provided Organic Substance Removal (OSR) Solution and Humic Acid Removal plate (HAR). Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR and qPCR  applications and more for any metagenomic study, as all humic acid substances and PCR inhibitors are removed during the isolation.

Details

Supporting Data

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Kit Specifications
Binding Capacity Per Well	50 μg
Maximum Loading Volume Per Well	500 μL
Size of DNA Purified	All sizes
Maximum Amount of Starting Material	250 mg
Time to Complete 96 Purifications	50 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 26560 (192 preps)
Lysis Buffer G	2 x 100 mL
Lysis Additive A	25 mL
Binding Buffer I	25 mL
OSR Solution	12 mL
Wash Solution A	2 x 38 mL
Elution Buffer B	30 mL
Bead Tubes	200
HAR Plate	2
96-Well Filter Plate	2
Adhesive Tape	4
96-Well Collection Plate	4
96-Well Elution Plate	2
Product Insert	1