Description
Specifications
| Clone | IHC583 |
| Source | Mouse Monoclonal |
| Positive Control | Breast Carcinoma, Urothelial Carcinoma |
| Dilution Range | 1:200 |
GATA3 is a transcription factor important in cell proliferation, development, and differentiation. GATA3 is mostly observed in breast and urothelial carcinomas, and rarely present in other cancers such as endometrial endometrioid adenocarcinoma. Among the breast carcinomas, GATA3 has a lower expression in luminal B subtype breast carcinoma. Studies have found GATA3 expression to be associated with ER (estrogen receptor), PR (progesterone receptor), and Her2 in breast cancer cases. Urothelial carcinomas stain positively for GATA3 in invasive or high grade tumors, therefore Anti-GATA3 is useful for carcinoma diagnosis when breast and bladder are plausible.
| Clone | IHC583 |
| Source | Mouse Monoclonal |
| Positive Control | Breast Carcinoma, Urothelial Carcinoma |
| Dilution Range | 1:200 |
Cryptosporidium is a parasite found in water that causes an infection in mammals termed cryptosporidiosis. It is one of the most common water-borne diseases and is found world-wide. It affects the intestines of mammals and typically causes an acute short-term infection. The most common symptom is self-limiting diarrhea in healthy individuals, however in immunocompromised individuals the symptoms are particularly severe and often fatal. There is no specific treatment for cryptosporidiosis other than fluid rehydration and management of any pain. Therefore early detection of Cryptosporidium in water is the foremost action to prevent the infection.
Cryptosporidium TaqMan RT-PCR Kit, 100 reactions
Cryptosporidium TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
Figure 1 / 3
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Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM39150 (100 preps) | Cat. TM39110 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| Cryptosporidium Primer & Probe Mix | 280 μL | 280 μL |
| Cryptosporidium Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
Magnetic Beads (tRNA Purification)
Solid Phase Reversible Immobilization magnetic beads has been used for nucleic acids purification because they are effective, simple, and fast. The carboxyl groups coated beads are paramagnetic particles that reversibly bind to nucleic acid. However, there is a drawback for magnetic beads that it can only purify DNA/RNA that are 100 base pairs or longer. tRNA and other DNA/RNA fragments shorter than 100 base pairs are not effectively recovered. We have developed Magnetic Beads (tRNA Purification) to overcome the hurdle.
The Magnetic Beads (tRNA Purification) solves the tRNA and short DNA/RNA purification problem. The beads with our proprietary technology purify tRNA effectively by removing impurities and unwanted components such as dNTPs, detergents, salts, proteins, and other contaminants. The magnetic bead reagents can be used for oligo (>70 nt) applications.
Workflow without large RNA/DNA contamination
In the case of samples contaminated with RNA/DNA such as rRNA and DNA, our magnetic beads can effectively remove RNA/DNA that are 180 nt and larger. Purified tRNA are ideal for applications requiring high quality, as the fragments are free of impurities and contaminants.
Workflow with large RNA/DNA contamination
Comparison of tRNA and 70 nt oligos recovery. BioDynami beads (tRNA purification) successfully recovered DNA fragments both yeast tRNA and 70 nt oligos.
Recovery of tRNA and 70 nt oligos with BioDynami magnetic Beads (tRNA Purification). Yeast tRNA and 70 nt oligos were used as input. Input and recovered oligos were quantified with ssDNA Quantification kit (BioDynami Cat. # 40043) and RNA Quantification kit (BioDynami Cat. # 40044).
Depletion of larger RNAs. Left panel: depletion of 28S rRNA and 18S rRNA. Right panel: depletion of RNA of 180 nt and larger.
Features
Removal of unwanted components and other impurities
Purification of tRNA and oligos (>70 nt)
tRNA
RNA fragments 70 nt or longer
DNA/RNA hybrid fragments 70 nt or longer
Oligo and chimeric oligo 70 nt or longer
dsDNA fragments 70 bp or longer
ssDNA fragments 70 nt or longer
Removal of larger RNA/DNA contamination:
18S rRNA
28S rRNA
RNA/DNA> 180 nt
Solid Phase Reversible Immobilization magnetic beads has been used for nucleic acids purification because they are effective, simple, and fast. The carboxyl groups coated beads are paramagnetic particles that reversibly bind to nucleic acid. However, there is a drawback for magnetic beads that it can only purify DNA/RNA that are 100 base pairs or longer. tRNA and other DNA/RNA fragments shorter than 100 base pairs are not effectively recovered. We have developed Magnetic Beads (tRNA Purification) to overcome the hurdle.
Norgen’s Stool Nucleic Acid Collection and Preservation System is a complete system designed for collection, ambient storage and transport of nucleic acids from stool specimens. Each of the 50 clamshells contain: nitrile gloves, alcohol swabs, Fe-Col® Collection Paper, and Norgen’s Stool Nucleic Acid Collection and Preservation Tube containing Norgen’s Stool Preservative in a liquid format. The user simply collects the stool specimen using the provided Fe-Col® Collection Paper, and then transfers the stool into the Stool Nucleic Acid Collection and Preservation Tube using the spoon attached to the lid. The sample is then mixed gently until the stool is well submerged under the preservative. The Stool Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Stool Preservative allow samples to be stored at room temperature for over 2 years for DNA and up to 7 days for RNA. To extend the stool RNA stability in the preservative, storage at -20°C or -70°C is recommended upon arrival at the testing facilities until RNA purification. The Stool Preservative also prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely.
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Tips and tricks for isolating high yield and quality DNA, RNA, miRNA and EV’s from fecal samplesDownload for Free
Figure 1 / 7
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| Kit Specifications | |
| Stability of Stool Nucleic Acids at Room Temperature | 2 years for DNA Up to 7 days for RNA* |
* The RNA stability will vary depending on the samples
Storage Conditions and Product Stability
All tubes should be kept tightly sealed and stored at room temperature (15 – 25°C) for up to 2 years without any reduction in performance.
| Kit Components | Cat. 63700 |
|---|---|
| Stool Nucleic Acid Collection and Preservation System | 50 |
| Product Insert | 1 |
| Individual Stool Nucleic Acid Collection and Preservation Device Contents | |
| Stool Nucleic Acid Collection and Preservation Tube | 1 |
| Nitrile Gloves | 2 |
| Fe-col® Collection Paper | 1 |
| Alcohol Swabs | 2 |
| Instruction Card | 1 |
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Tel : 081-875-1869 , 02-328-7179
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