Description
Specifications
| Clone | IHC583 |
| Source | Mouse Monoclonal |
| Positive Control | Breast Carcinoma, Urothelial Carcinoma |
| Dilution Range | 1:200 |
GATA3 is a transcription factor important in cell proliferation, development, and differentiation. GATA3 is mostly observed in breast and urothelial carcinomas, and rarely present in other cancers such as endometrial endometrioid adenocarcinoma. Among the breast carcinomas, GATA3 has a lower expression in luminal B subtype breast carcinoma. Studies have found GATA3 expression to be associated with ER (estrogen receptor), PR (progesterone receptor), and Her2 in breast cancer cases. Urothelial carcinomas stain positively for GATA3 in invasive or high grade tumors, therefore Anti-GATA3 is useful for carcinoma diagnosis when breast and bladder are plausible.
| Clone | IHC583 |
| Source | Mouse Monoclonal |
| Positive Control | Breast Carcinoma, Urothelial Carcinoma |
| Dilution Range | 1:200 |
t-Boc-aminooxy-PEG5-propargyl is a click crosslinker. Propargyl group is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. T-Boc-aminooxy can be converted to free aminooxy under mild acidic conditions and then can react with an aldehyde or ketone. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
t-Boc-aminooxy-PEG5-propargyl is a click crosslinker. Propargyl group is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. T-Boc-aminooxy can be converted to free aminooxy under mild acidic conditions and then can react with an aldehyde or ketone. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This kit provides a simple and fast solution for the extraction of circulating nuclear acid from serum, plasma, and other cell-free liquid samples. Circulating nucleic acid refers to the free extracellular nucleic acid produced by cell apoptosis, of which fragments are generally below 1KB. The kit is based on silica gel column purification technology, which is no need for toxic phenol chloroform extraction and time-consuming alcohol precipitation during the extraction. The obtained Circulating Nucleic Acid can be directly used for quantitative PCR, liquid or solid phase chip analysis, hybridization, and SNP detection.
HiPure Circulating DNA/RNA Kit adopts a unique solution system and multiple layers of filter membranes with different pore sizes, which can efficiently process large volumes of serum and plasma samples and capture extremely small amounts of free nucleic acids.
Specifications
| Features | Specifications |
| Main Functions | Isolation both Circulating DNA/RNA (include miRNA) from 1-5 ml serum and plasma |
| Applications | qPCR / RT-PCR, liquid or solid-phasechip analysis, hybridization and SNP detection |
| Purification method | Midi spin column |
| Purification technology | Silica technology, DNA filtration technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | serum, plasma, and other cell-free liquid samples |
| Sample amount | 1-5 ml |
| Elution volume | ≥20μl |
| Time per run | ≤100 minutes |
| Liquid carrying volume per column | 4 ml |
| Binding yield of column | 1 mg |
This kit is based on silica gel column technology. Serum or other liquid samples are lysed and digested in buffer CFL. After adding buffer CFP, the protein is removed by centrifugation to obtain the supernatant. Isopropanol is added to precipitate the total nucleic acid and transferred to the column for filtration. DNA / RNA is adsorbed on the membrane of the column, while the protein is not adsorbed and removed with the filtrate.The column is washed with buffer MGW1 to remove protein and other impurities, and then washed with buffer RW2 to remove salt. Finally, DNA / RNA is eluted by low salt buffer. The eluted DNA / RNA can be directly used for quantitative PCR/ RT-PCR, liquid or solid-phase chip analysis, hybridization and SNP detection.
Advantages
Kit Contents
| Contents | R431602 | D431603 |
| Purification Times | 50 Preps | 250 Preps |
| HiPure RNA Micro Columns | 50 | 5 x 50 |
| HiPure Viral Midi Columns | 50 | 5 x 50 |
| 15 ml Collection Tubes | 50 | 5 x 50 |
| 2ml Collection Tubes | 50 | 5 x 50 |
| Buffer CFL | 150 ml | 2 x 375 ml |
| Buffer CFP | 30 ml | 150 ml |
| Buffer MGW1* | 100 ml | 2 x 250 ml |
| Buffer RW2* | 2 x 50 ml | 5 x 100 ml |
| RNase Free Water | 10 ml | 50 ml |
Storage and Stability
The kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
This kit provides a simple and fast solution for the extraction of circulating nuclear acid from serum, plasma, and other cell-free liquid samples. Circulating nucleic acid refers to the free extracellular nucleic acid produced by cell apoptosis, of which fragments are generally below 1KB. The kit is based on silica gel column purification technology, which is no need for toxic phenol chloroform extraction and time-consuming alcohol precipitation during the extraction. The obtained Circulating Nucleic Acid can be directly used for quantitative PCR, liquid or solid phase chip analysis, hybridization, and SNP detection.
HiPure Circulating DNA/RNA Kit adopts a unique solution system and multiple layers of filter membranes with different pore sizes, which can efficiently process large volumes of serum and plasma samples and capture extremely small amounts of free nucleic acids.
An enhanced PCR master mix for allele-specific assays. Improved signal to noise ratio and tight clustering. Developed specifically for genotyping direct from crude DNA samples.
PACE 2.0 Genotyping Master Mix ensures an unrivalled signal-to-noise ratio and produces tight data clusters, even when working with high-throughput, crude DNA preps, resulting in consistently exceptional performance. Efficiently streamline your workflow and reduce costs without compromising the quality of your results.
PACE 2.0 Genotyping Master Mix is an ideal solution for challenging starting material. PACE 2.0 has been specially formulated to overcome the obstacles presented by common PCR inhibitor compounds, such as phenols and tannins. Even notoriously tricky samples like oil palm and conifers can still be assayed using hot shot or other crude DNA prep methods and deliver reliable and accurate data.
PACE 2.0 Genotyping Master Mix uses a novel, universal, fluorescent reporting cassette to produce machine-readable fluorescent signals corresponding to genotypes. PACE 2.0 compatible genotyping assays are comprised of two competitive allele-specific forward primers (which differ in their terminal 3’ bases and unique 5’ tail sequences) and a common, reverse primer. PACE 2.0 Genotyping Master Mix is supplied with ROX normalising dye at a range of levels to ensure compatibility with your qPCR instrument or reader.
Genotyping assay designs are available from 3CR Bioscience through our free assay-design service; once designed, users can purchase assay primers independently or through 3CR Bioscience using our partial or full-assay validation service. PACE 2.0 Genotyping Master Mix is also compatible with KASP™ and Amplifluor® marker assays.