Description
Specifications
| Clone | IHC623 |
| Source | Mouse Monoclonal |
| Positive Control | Breast, Colon, Associated Adenocarcinomas |
| Dilution Range | 1:200 |
Mucin 1 (MUC1) is a membrane-bound glycoprotein involved in a number of protective and cell-signaling functions, including cell-cell adhesion, proliferation, motility, invasion, and survival. Overexpression of MUC1 is clinically indicated in breast carcinomas, papillary thyroid carcinomas, and thymic carcinomas, and reports have named MUC1 as a useful marker for differentiating thymic carcinoma from type B3 thymoma. The expression of MUC1 is correlated with the grade of malignancy in thymic epithelial tumors, and loss of MUC1 expression has been associated with reactive gastropathy. MUC1 is not expressed in normal human epidermis, but has been detected in the epidermis of psoriatic plaques of biopsies from patients diagnosed with psoriasis vulgaris.
| Clone | IHC623 |
| Source | Mouse Monoclonal |
| Positive Control | Breast, Colon, Associated Adenocarcinomas |
| Dilution Range | 1:200 |
Propargyl-PEG12-methylamine is a propargyl linker that is commonly used as a Click Chemistry reagent for copper-catalyzed reactions with azides. The methylamine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde), etc. The PEG spacer helps improve the water solubility of the molecule in aqueous media. Reagent grade, for research use only.
Propargyl-PEG12-methylamine is a propargyl linker that is commonly used as a Click Chemistry reagent for copper-catalyzed reactions with azides. The methylamine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde), etc. The PEG spacer helps improve the water solubility of the molecule in aqueous media. Reagent grade, for research use only.
West Nile Virus (WNV) belongs to the RNA virus family of Flaviviridae. It is known to spread primarily through arthropod vectors such as mosquitoes. The virus infects mainly birds but is also reported to infect humans as well as pets such as cats and dogs. In humans, the majority of the infections caused by WNV are asymptomatic. However, some individuals (the majority of the confirmed cases) could enter a second, febrile stage with flu-like symptoms – commonly known as West Nile Fever. In a more serious stage, the disease becomes neuroinvasive, causing meningitis or encephalitis. Such severe conditions could lead to mortality. As the symptoms of WNV are very similar to other common diseases but can be fatal at a severe stage, it is important to distinguish the virus early in the diagnosis, particularly at the molecular level.
WNV TaqMan RT-PCR Kit, 100 reactions
WNV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
Figure 1 / 3
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Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM44250 (100 preps) | Cat. TM44210 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| WNV Primer & Probe Mix | 280 μL | 280 μL |
| WNV Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
Mal-amido-PEG4-alkyne is a short PEG linker featuring a maleimide and a terminal alkyne. Maleimide is a covalent ligand used for linking thiols such as those in cysteines residues in proteins, while terminal alkynes can be used in copper (I) click chemistry with azides on target molecules.
Mal-amido-PEG4-alkyne is a short PEG linker featuring a maleimide and a terminal alkyne. Maleimide is a covalent ligand used for linking thiols such as those in cysteines residues in proteins, while terminal alkynes can be used in copper (I) click chemistry with azides on target molecules.