Description
Specifications
| Clone | IHC026 |
| Source | Rabbit Monoclonal |
| Positive Control | Colon Cancer |
| Dilution Range | 1:200 |
MutS Homolog 6 (MSH6) is a protein involved in the mismatch repair pathway. This protein is commonly associated with hereditary non-polyposis colorectal cancer, and mutations in this gene are correlated with the development of sporadic colorectal carcinoma. Studies have shown that mutations in MSH6, when co-indicated with mutations in MSH1 and MSH2, contribute to the development of sporadic colorectal carcinoma. Use of Anti-MSH2 is optimized when paired with MSH6, MLH1, and PMS2 in an IHC panel.
| Clone | IHC026 |
| Source | Rabbit Monoclonal |
| Positive Control | Colon Cancer |
| Dilution Range | 1:200 |
t-Boc-N-Amido-PEG5-propargyl comprises a propargyl group and Boc-protected amine. The propargyl group reacts with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The Boc group can be deprotected under mild acidic conditions to form the free amine. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
t-Boc-N-Amido-PEG5-propargyl comprises a propargyl group and Boc-protected amine. The propargyl group reacts with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The Boc group can be deprotected under mild acidic conditions to form the free amine. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The Flex caddy allows the Temperature Module GEN2 to be installed on the Opentrons Flex deck and sit below the deck, enabling below-deck cable routing and labware placed on top of modules to remain closer to the deck surface. The calibration adapter is used to calibrate the location of the Temperature Module. Caddies and calibration adapters are specific to the type of module.
Note: This item does not include the Temperature Module. The module is available with or without the Flex Caddy and Calibration tool: Temperature Module GEN2
The Flex caddy allows the Temperature Module GEN2 to be installed on the Opentrons Flex deck and sit below the deck, enabling below-deck cable routing and labware placed on top of modules to remain closer to the deck surface. The calibration adapter is used to calibrate the location of the Temperature Module. Caddies and calibration adapters are specific to the type of module.
Note: This item does not include the Temperature Module. The module is available with or without the Flex Caddy and Calibration tool: Temperature Module GEN2
HiPure DNA Clean Up 96 Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Specifications
| Features | Specifications |
| Main Functions | Recover DNA fragments between 60bp-10kbp from PCR, Reactions, crude DNA using 96 well Bind Plate |
| Applications | PCR, sequencing, labeling reactions, ligations and restriction digestion, etc. |
| Purification method | 96 well Bind Plate |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | PCR product, enzymatic reaction |
| Sample amount | Appropriate |
| Recovery | ≥80% |
| Elution volume | ≥75μl |
| Time per run | ≤60 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 20µg |
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
Kit Contents
| Contents | D212201 | D212202 | D212203 |
| Purification Times | 1 x 96 Preps | 4 x 96 Preps | 20 x 96 Preps |
| Buffer GDP | 120 ml | 400 ml | 4 x 400 ml |
| Buffer DW2 | 20 ml | 100 ml | 3 x 100 ml |
| Elution Buffer | 20 ml | 20 ml | 30 ml |
| HiPure DNA Plate | 1 | 4 | 20 |
| 2.2ml Collection Plate | 1 | 4 | 20 |
| 1.6 ml Collection Plate | 1 | 4 | 20 |
| 0.8 ml Collection Plate | 1 | 4 | 20 |
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.
HiPure DNA Clean Up 96 Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.