GeneAb™ MSH6

Description 

The G-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase suitable for GC-rich templates that are difficult to amplify. The fidelity of G-HiFi™ DNA Polymerase is 70 times higher than that of Taq DNA polymerase. The high extension rate of G-HiFi™ DNA Polymerase is achieved by blending the DNA polymerase with an elongation enhancer. The optimized 5X G-HiFi™ Buffer includes special ingredients that suppress non-specific amplification as well as plateau effect produced by conventional PCR. With the optimized 5X G-HiFi™ Buffer, G-HiFi™ DNA Polymerase is capable to amplify most templates, such as longer targets (up to 40 kb from lambda DNA) and that contain GC-rich sequences. 

Features

• 5’→3’ DNA polymerase activity 
• 3’→5’ exonuclease (proofreading) activity
• Suitable for GC-rich templates 
• High reaction rate: 7 seconds/kb 
• High fidelity: 70 times higher than Taq polymerase 
• Generates blunt end amplicons  
• Vast elongation capability (up to 40 kb) 
• Thermo-stable for more than 10 hrs at 95°C 

Storage

[TF3000] G-HiFi™ DNA Polymerase

-20°C for 24 months

The G-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase suitable for GC-rich templates that are difficult to amplify. The fidelity of G-HiFi™ DNA Polymerase is 70 times higher than that of Taq DNA polymerase. The high extension rate of G-HiFi™ DNA Polymerase is achieved by blending the DNA polymerase with an elongation enhancer. The optimized 5X G-HiFi™ Buffer includes special ingredients that suppress non-specific amplification as well as plateau effect produced by conventional PCR. With the optimized 5X G-HiFi™ Buffer, G-HiFi™ DNA Polymerase is capable to amplify most templates, such as longer targets (up to 40 kb from lambda DNA) and that contain GC-rich sequences.

Overview

• Protocol optimized for DNA isolated from a diversity of samples including stool, soil, water, saliva, plant, urine, skin, and more
• Simple and quick workflow: library could be prepared in less than 4 hours
• Component of Norgen’s metagenomics workflow
• A single NGS run can be prepared with up to 384 unique dual-index libraries

Sample type purification kit guide 

The ITS2 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the fungal target ITS2 libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XPMagneticBeads(supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.

The ITS2 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the ITS2 region of the fungal DNA. The post-PCR reaction is then cleaned up using AMPure XPbeads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XPbeads. The libraries are then ready for quantification, pooling and sequencing.

Details

Supporting Data

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Minimum amount of starting material:	2.5 µL of DNA (5 ng/µL)
Time to complete library preparation:	4 hours

Storage Conditions and Product Stability
Norgen’s ITS2 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.

All kit components will remain stable for at least 1 year when stored at the specified storage conditions.

Features:

1. Programmable microprocessor control system, touch screen indicates rotor No., speed, time, temperature, RCF, rate of speed as well as fault information.  

2. Adopts band heater, fast heating speed, temperature uniform, with the function of temperature-control and constant.

3. Centrifugal tube made in special skill, anti-broken when work.

4. Great toque brushless motor, maintenance-free, no powder, fast acceleration and deceleration, low noise, which improve the stability a lot.

5. Electric lid lock, over-speed and over-temperature protection

6.3 phase to keep the best performance.

7. Stainless steel chamber, safe and reliable

TD5B Technical Parameter：

Max. Speed	4000rpm
Max. RCF	2498×g
Max. Capacity	4×100ml
Time Range	0~99min
Rotor	Swing   Out Rotor
Noise (dB)	≤ 58
Temperature	Room   temperature:+10~80℃
Acc/Dcc	10 Kinds
Speed Accuracy	±20r/min
Temperature   Accuracy	±1℃
Oil tube size	37×200mm
Size (W x D x Hmm)	685×500×385mm
Net Weight(Kg)	67KG

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