Overview

• Simultaneous isolation of both host DNA and microbial DNA (universal protocol)
• Fully compatible with Norgen’s Stool Nucleic Acid Collection and Transport Tubes
• Eliminates PCR inhibitors including humic acids
• High quality DNA for sensitive downstream applications including PCR, qPCR, Sequencing and microarray
• 96-Well and Magnetic Bead System formats also available

These kits provide a convenient and rapid method to isolate total DNA from fresh, frozen and preserved stool samples, including those preserved using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The universal protocol conveniently allows for the isolation of total genomic DNA from all the various microorganisms and host cells found in the stool sample simultaneously. Purifies the DNA with high yields and molecular weights of up to 50 kb plus. The purified DNA can be used with a number of downstream applications.

Stool DNA Isolation Kit (Spin Column)

Universal method to detect microorganism and host cell DNA simultaneously in stool samples. Eliminates PCR inhibitors including all traces of humic acid using a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.

Stool DNA Isolation 96-Well Kit (High Throughput)

Fast and easy high throughput processing using centrifugation. Eliminates PCR inhibitors including all traces of humic acid using a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.

Stool DNA Isolation Kit (Magnetic Bead System)

Fast and easy processing using a magnetic bead system. Robust lysis system (chemical lysis combined with a mechanical homogenization).

Stool DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)

This kit provides fast and easy processing using a magnetic bead system, and a robust lysis system (chemical lysis combined with a mechanical homogenization). High throughput and compatible with an automation robotic system.

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Details

Supporting Data

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Kit Specifications
Maximum Stool Input	200 mg (fresh/frozen stool) or 400 μL (preserved stool)
Type of Stool Processed	Frozen, fresh or preserved stool
Format	Spin Column
Maximum Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Elution Volume	50 μL
Time to Complete 10 Purifications	30 minutes
Applications	PCR, qPCR, Southern Blot Analysis, Sequencing, Microarray Analysis.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 27600 (50 preps)	Cat. 65600 (192 preps)	Cat. 55700 (50 preps)	Cat. 63100 (192 preps)
Lysis Buffer L	60 mL	2 x 105 mL	60 mL	3 x 60 mL 1 x 30 mL
Lysis Additive A	6 mL	25 mL	6 mL	25 mL
Binding Buffer I	7 mL	25 mL	7 mL	25 mL
Binding Buffer C	30 mL	110 mL	–	–
Wash Solution A	18 mL	2 x 38 mL	–	–
Magnetic Bead Suspension	–	–	1.1 mL	4 x 1.1 mL
Solution WN	–	–	18 mL	55 mL
Elution Buffer B	8 mL	30 mL	8 mL	30 mL
Bead Tubes	50	200	50	196
Mini Spin Columns	50	–	–	–
96-Well Plate	–	2	–	2
Adhesive Tape	–	4	–	2
Collection Tubes	50	–	–	–
96-Well Collection Plate	–	2	–	–
Elution Tubes (1.7 mL)	50	–	50	–
96-Well Elution Plate	–	2	–	2
Product Insert	1	1	1	1

Introduction

This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 100 mg of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic,mitochondrial, and chloroplast) for reliable PCR and southern blotting in less than 1 hour. Purification requires no phenol or chloroform extraction oralcohol precipitation and involves minimal handling.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from100 mg simple plant
Applications	PCR, SSR, AFLP, RAPD and southern blot, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Economic plant samples
Sample amount	Fresh / frozen plant samples: ≤100mggDried plant / seed samples: ≤20mg
Elution volume	≥30μl
Time per run	30-50 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Principle

Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated.Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.

Advantages

• Safe – require no phenol or chloroform extraction
• Fast – several samples can be extracted in 30 minutes by silica technology
• High purity – high quality DNA, completely remove inhibitors
• High yield – silica technology can achieve the highest yield

Kit Contents

Contents	D316402	D316403
Purification Times	50 Preps	250 Preps
RNase A	10 mg	50 mg
Protease Dissolve Buffer	1.8 ml	5 ml
Buffer SPL	30 ml	150 ml
Buffer PS	10 ml	50 ml
Buffer GW1	22 ml	110 ml
Buffer GW2*	12 ml	2 x 50 ml
Buffer AE	15 ml	60 ml
HiPure gDNA Mini Columns	50	2 x 125
2 ml Collection Tubes	50	2 x 125

Storage and Stability

RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Experiment Data

Purchase Guide

Name	CAT NO	Fresh / frozen tissue amount	Dried tissue amount	Column type	Elution volume	Yield (muscle)
HiPure Plant DNA Mini Kit	D3187	150mg	40mg	1.5ml column	50 – 100μl	3-70μg
HiPure HP Plant DNA Maxi Kit	D3163	5g	1g	50ml column	0.7 – 3ml	75-1250μg
HiPure SF Plant DNA Kit	D3164	100mg	20mg	1.5ml column	50 – 100μl	3-50μg
HiPure SF Plant DNA 96 Kit	D3167	50mg	15mg	96 well plate	75 – 150μl	3-30μg

This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 100 mg of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic,mitochondrial, and chloroplast) for reliable PCR and southern blotting in less than 1 hour. Purification requires no phenol or chloroform extraction oralcohol precipitation and involves minimal handling.

K-MRGLUC

1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser)

Content:	1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	D-Glucose
Assay Format:	Spectrophotometer, Microplate, Auto-analyser
Detection Method:	Absorbance, Fluorescence
Wavelength (nm):	(Fluorescence) 530-560 (ex), ~ 590 (em) (Absorbance) 570
Signal Response:	Increase
Linear Range:	(Fluorescence) 0.009-0.18 μg of D-glucose per assay (0.5-10 μM in-assay) (Absorbance) 0.9-9.0 μg of D-glucose per assay (5-50 μM in-assay)
Limit of Detection:	(Fluorescence) 0.09 ug/mL (Absorbance) 0.25 ug/mL
Reproducibility (%):	(Fluorescence) ~ 6% (Absorbance) ~ 7%
Reaction Time (min):	(Fluorescence) 30 min (Absorbance) 30 min
Application examples:	Food and beverage samples such as wine, beer, fruit juices, soft drinks, milk, jam, dietetic foods, bakery products, candies, fruit and vegetables. Other samples such as tobacco, cosmetics, pharmaceuticals (e.g. infusions), feed, paper (and cardboard) and other materials (e.g. biological cultures, samples, etc.).

This product has been discontinued.

The D-Glucose Assay Kit (Megaplex Red) provides a simple robust method for the measurement of D-glucose. This kit utilises a highly sensitive Megaplex Red probe which when coupled to horseradish peroxidase (HRP) and glucose oxidase (GOX) enzymatic reactions, allows for the measurement of D-glucose in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation between 530-560 nm and emission at ~ 590 nm* or colourimetrically at 570 nm.

*The excitation and emission maxima of Megaplex Red are 570 nm and 585 nm respectively.

See more related mono/disaccharide assay kit products. 

Fluorometric/UV method for the determination of D-glucose content in a variety of samples.

Advantages

• Simple format
• Very competitive price (cost per test)
• Ability to run in fluorescence or absorbance mode
• User friendly – Detailed protocol provided for the creation of calibration curve and the calculation of concentration in fluorescence mode. Single point standard for quicker and simpler analysis in absorbance mode.
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
• Suitable for manual, microplate and auto-analyser formats
• All reagents stable for > 2 years

The D-Glucose Assay Kit (Megaplex Red) provides a simple robust method for the measurement of D-glucose. This kit utilises a highly sensitive Megaplex Red probe which when coupled to horseradish peroxidase (HRP) and glucose oxidase (GOX) enzymatic reactions, allows for the measurement of D-glucose in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation between 530-560 nm and emission at ~ 590 nm* or colourimetrically at 570 nm.