GeneAb™ TFE3

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Applications

• Viral Bioprocessing
• Complete Removal of DNA

M-SAN HQ  – Peak performance where it matters most at physiological conditions

Medium-Salt Active Nuclease High Quality (M-SAN HQ) is a Bioprocessing Grade nuclease developed for removal of both single and double-stranded DNA and RNA at the physiological salt conditions most often used in bioprocessing and biomanufacturing workflows. M-SAN HQ allows you to directly replace Benzonase without changing your workflow.

This novel, nonspecific endonuclease is active over a broad pH range and displays optimum activity at salt concentrations between 125 – 250 mM. Due to its excellent performance at physiological conditions, M-SAN HQ can be used directly in the cell medium or the harvested supernatant without buffer adjustments. This makes M-SAN HQ ideal for the manufacturing of fragile vectors such as lentiviruses and retroviruses.

M-SAN HQ can be directly used in medium without buffer adjustments The high activity of M-SAN HQ at standard cell medium conditions leads to improved DNA clearance compared to other commonly used nucleases. In the data (see Figure 6), an over 2-fold reduction in residual DNA was achieved. 

Key Benefits

• Compatibility: Ideal for working with both fragile and robust viral vectors and proteins in a variety of cell media
• Optimum activity: Optimization for cell media salinity allows both shorter DNA fragments and reduced incubation times.
• Cost-Effectiveness: Reduced need for additional reagents and steps can lower production costs
• Quality: Maintains the integrity of sensitive or labile biological molecules, ensuring a higher quality end product
• Flexibility: Can be used directly in a variety of media without the need for customization

Key Features 

• High purity (≥ 99%)
• No protease detected
• Endotoxin-tested
• Animal origin-free production
• Supplied with extended product documentation

Adapted to use in medium without salinity adjustments 

Optimal activity at physiological salinity and pH makes it ideal for DNA removal from mammalian cell media. (see Figures)

The high activity of M-SAN HQ at the physiological salinity and pH found in standard cell medium conditions leads to improved DNA clearance compared to commonly used nucleases.

Simple to Optimize in Cell Media

M-SAN HQ is uniquely formulated to excel in physiological pH conditions, offering high performance at the commonly used cell media pH of 7.4.

While other nucleases often require more alkaline environments, M-SAN HQ stands out for its adaptability and effectiveness in cell media. It’s the nuclease that truly aligns with your bioprocessing needs. (See Figure section)

M-SAN HQ ELISA Kit

The M-SAN HQ ELISA Kit confirms the removal of M-SAN High Quality in bioprocessing and biomanufacturing applications with high accuracy: 

• 100% (±10%) Measuring range 0.47 – 7.50 ng/ml
• 100% (±20%) Measuring range 0.12 – 0.47 ng/ml

Features:

Shelf life: 18 months

Fast: 1.5 – 2 hours

Sensitive – Quantification range: 0.12 – 7.5 ng/ml

Accurate

Flexible – 96-well plate in 8-well strip format

Medium-Salt Active Nuclease High Quality (M-SAN HQ) is a Bioprocessing Grade nuclease developed for removal of both single and double-stranded DNA and RNA at the physiological salt conditions most often used in bioprocessing and biomanufacturing workflows. M-SAN HQ allows you to directly replace Benzonase without changing your workflow.

Permagen’s 0.2 mL PCR / 1.5 mL Microcentrifuge tube separation rack is designed for labs with both PCR strip and 1.5 mL separation protocols. On the top, hold up to two of either 8 or 12 strips, or up to 24 individual tubes. Flip over and hold up to twelve 1.5 mL Microcentrifuge Tubes

Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, rubber feet on both sides to help prevent slipping on work bench, less tippy than common plastic products, and fast separations using any magnetic beads

SKU #

MSR1224B

Features

• Aluminum alloy design for years of trouble-free use
• Fast magnetic bead separations
• Held to tighter tolerances for more consistent results
• Pulls beads to side of wells for easy tip tracking down opposite side
• Rubber feet to help prevent slippage
• Stable design
• Angled tube holes which allow the tubes to come close to magnets

Compatibility

• Most 1.5 mL Microcentrifuge tubes
• Some 2.0 mL Microcentrifuge tubes
• 8-strip PCR tubes
• 12-strip PCR tubes
• Individual PCR tubes 
• Any Magnetic Beads

Volumes

Minimum Volume 0.2 mL PCR Tube – 10 µL

Maximum Volume 0.2 mL PCR Tube – 0.2 mL

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Minimum Volume 1.5 mL Microcentrifuge Tube – .5 mL

Maximum Volume 1.5 mL Microcentrifuge Tube – 1.5 mL

Permagen’s 0.2 mL PCR / 1.5 mL Microcentrifuge tube separation rack is designed for labs with both PCR strip and 1.5 mL separation protocols. On the top, hold up to two of either 8 or 12 strips, or up to 24 individual tubes. Flip over and hold up to twelve 1.5 mL Microcentrifuge Tubes

K-FRUCHK

SKU: 700004286

50 assays per kit

Content:	50 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Fructan
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	4 to 80 µg of D-glucose, D-fructose or sucrose per assay
Limit of Detection:	1 g/100 g
Total Assay Time:	~ 30 min
Application examples:	Flours, plant materials (e.g. onion), food products and other materials

The Fructan HK test kit is suitable for the specific measurement and analysis of all fructo-oligosaccharides (reducing and non-reducing) and of fructan polysaccharides but is not suitable for the analysis of samples containing high levels of D-glucose, D-fructose, sucrose or maltose.

View our full range of assay kits for polysaccharides.

Advantages

• Very cost effective 
• All reagents stable for > 12 months after preparation 
• Fructan kits are available only from Megazyme 
• Simple format 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included

The Fructan HK test kit is suitable for the specific measurement and analysis of all fructo-oligosaccharides (reducing and non-reducing) and of fructan polysaccharides but is not suitable for the analysis of samples containing high levels of D-glucose, D-fructose, sucrose or maltose.