GeneAb™ Tau

Overview

• Detection kits for Mycoplasma DNA
• Available in TaqMan format for analysis

Mycoplasma is a common and serious contaminant of cell cultures. Mycoplasma is often not visible and does not respond to antibiotics, and therefore it is a major issue that requires monitoring and early detection. Up to 30% of cell cultures may be contaminated with Mycoplasmas, the main contaminants being the species M. orale, M. laidlawii, M. arginini and M. hyorhinis. These organisms produce a variety of effects on the infected cells in culture, including changes in metabolism growth, viability and morphology, thereby altering the phenotypic characteristics of the host cells. Therefore there is an absolute requirement for routine, periodic assays to diagnose possible Mycoplasma contamination of all cell cultures, particularly continuous or established cell lines.

Mycoplasma TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Mycoplasma TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM33150 (100 preps)	Cat. TM33110 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
Mycoplasma Primer & Probe Mix	280 μL	280 μL
Mycoplasma Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Overview

• The preservative provides sample homogeneity eliminating sample variability
• Preserve and transport DNA & RNA safely at ambient temperature
• Nucleic acid preservation at room temperature over 2 years for DNA and 7 days for RNA
• No cold shipping/storage needed – hassle-free and cost effective
• Isolate nucleic acids for any application including 16s NGS
• Robust preservation over a range of temperatures
• Customizable with various accessories for easy and safe collection
• Eliminates odor and renders samples safe and non-infectious

Norgen’s Stool Nucleic Acid Collection and Preservation Tubes are designed for the rapid preservation of nucleic acids from fresh stool specimens. The Stool Nucleic Acid Collection and Preservation Tubes contain Norgen’s Stool Preservative in a liquid format. The user simply collects stool into the tubes (fill up to the line indicated on the tube) and mixes gently until the stool is well submerged under the preservative. The Stool Preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the Stool Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Stool Preservative allow samples to be stored at room temperature for over 2 years for DNA and 7 days for RNA. To extend the stool RNA stability in the preservative, storage at -20°C or -70°C is recommended at the arrival to testing facilities until the RNA purification.

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Extracting Biological Insights from Stool

Tips and tricks for isolating high yield and quality DNA, RNA, miRNA and EV’s from fecal samplesDownload for Free

Details

Supporting Data

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Kit Specifications
Stool Input	2 g
Stability of Stool Nucleic Acids at Room Temperature	2 years for DNA 7 days for RNA*

* The RNA stability will vary depending on the samples

Storage Conditions and Product Stability

All tubes should be kept tightly sealed and stored at room temperature (15 – 25 °C) for 2 years after the date of shipment without any reduction in performance.

Kit Components	Cat. 45660
Stool Nucleic Acid Collection and Preservation Tubes	50
Product Insert	1

K-CATAL

SKU: 700004271

100 / 200 assays per kit

Content:	100 / 200 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Catalase
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	520
Signal Response:	Increase
Limit of Detection:	0.5 U/mL
Total Assay Time:	20 min
Application examples:	Various food, biological and bacterial samples.
Method recognition:	Novel method

Megazyme’s catalase assay kit provides a simple colourimetric method for the measurement of catalase activity. The method is delivered in a fast and reliable format and may be used to detect catalase activity in various samples, including food, biological and bacterial samples. The calculation method included allows for significant variation in the concentration of the H₂O₂ substrate solution that is employed in the assay which translates into a reduction in the ‘hands on’ time required by the analyst in comparison to other commercial kits.

See our complete list of assay kits for the measurement enzyme activity.

Advantages

• Very cost effective 
• Simple, convenient, rapid assay 
• Standard included  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing

Megazyme’s catalase assay kit provides a simple colourimetric method for the measurement of catalase activity. The method is delivered in a fast and reliable format and may be used to detect catalase activity in various samples, including food, biological and bacterial samples. The calculation method included allows for significant variation in the concentration of the H2O2 substrate solution that is employed in the assay which translates into a reduction in the ‘hands on’ time required by the analyst in comparison to other commercial kits.