Introduction

This product allows rapid and reliable isolation of high-quality genomic DNA from various soil and stool samples. Up to 100mg stool sample and 500 mg soil samples can be processed in 60 minute. The system combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of spin column technology to eliminate PCR inhibiting compounds such as humic acid from soil samples. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed inparallel.

Details

Specifications

Features	Specifications
Main Functions	Isolation DNA from 200-500mg soil, 50-100mg stool, or 100-500mg other environmental samples using 96 plate
Applications	PCR, southern blot and enzyme digestion, etc.
Purification method	96 well plate
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Soil, stool, other environmental samples
Sample amount	Soil: 200-500mgStool: 50-100mgOther environmental samples: 100-500mg
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column

Principle

Soil/Stool sample is homogenized and then treated in a specially formulated buffer containing detergent to lyse bacteria, yeast, and fungal samples. Humic acid, proteins, polysaccharides, and other contaminants are removed using our proprietary Absorber Solution. Binding conditions are then adjusted and the sample is applied to a DNA Mini Column. Two rapid wash steps remove trace contaminants and pure DNA is eluted in low ionic strength buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

Advantages

• Fast – several samples can be extracted in 40 minutes (after digestion)
• High purity – the purified DNA can be directly used in various downstream applications
• Good repeatability – silica technology can obtain ideal results every time
• High recovery – DNA can be recovered at the level of PG

Kit Contents

Contents	D314401	D314402	D314403
Purification Times	1 x 96 Preps	4 x 96 Preps	20 x 96 Preps
HiPure DNA Plate	1	4	20
96 Well Plate (2.2ml)	1	4	20
1.6ml Collection Plate	1	4	20
0.8ml Collection Plate	1	4	20
2ml Bead Tubes	100	400	2000
Buffer SOL	100 ml	360 ml	2 x 900 ml
Buffer SDS	10 ml	36 ml	180 ml
Reagent DX	1 ml	1.8 ml	9 ml
Buffer PS	20 ml	80 ml	400 ml
Absorber Solution	20 ml	80 ml	400 ml
Buffer GDP	150 ml	500 ml	3 x 900 ml
Buffer GW2*	50 ml	100 ml	4 x 200 ml
Buffer AE	30 ml	120 ml	500 ml

Storage and Stability

Absorber Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

This product allows rapid and reliable isolation of high-quality genomic DNA from various soil and stool samples. Up to 100mg stool sample and 500 mg soil samples can be processed in 60 minute. The system combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of spin column technology to eliminate PCR inhibiting compounds such as humic acid from soil samples. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed inparallel.