Discover our Tryptose Phosphate Broth, formulated to support the cultivation of fastidious microorganisms, including Haemophilus influenzae and Neisseria species. Ideal for microbiological research……
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Discover our Tryptose Phosphate Broth, formulated to support the cultivation of fastidious microorganisms, including Haemophilus influenzae and Neisseria species. Ideal for microbiological research, pharmaceutical testing, and vaccine production.
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Hepatitis C Virus (HCV) TaqMan RT-PCR Detection Kits
Product Info
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Product Info
Overview
Detection kits for HCV
Available in TaqMan format for analysis
Hepatitis C virus infection is a major cause of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma (HCC) worldwide. Neither a vaccine nor an effective treatment is available for HCV. Pegylated interferon-α (PEG-IFN- α), combined with ribavirin, is the current standard therapy. Its efficiency ranges between 20-80%, depending on the HCV genotype. Unfortunately, many HCV infected patients do not respond to or do not tolerate the IFN-based therapy. Therefore, the number of patients progressing to HCC, as a result of HCV infection, is expected to increase over the next 20-30 years. The natural history of the disease reveals the elusive nature of the virus in a number of features. First, the infection is often detected incidentally at the time of blood donation as the acute infection is clinically asymptomatic in most patients. Second, HCV successfully escapes multi-specific host immune responses in the majority of patients which establishes persistent infection. Third, a significant number of persistently infected individuals remain unaware of the infection for decades, until liver fibrosis, cirrhosis and/or hepatocellular carcinoma develop.
HCV TaqMan RT-PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
HCV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
ArcticZymes’ Shrimp Alkaline Phosphatase (SAP), including its recombinant version (rSAP), is the gold standard and the first heat-labile, all-purpose alkaline phosphatase on the market.
Originally isolated from Pandalus borealis (Arctic shrimp), it has been purified from a recombinant source since 2010. Unlike other alkaline phosphatases, rSAP can be fully inactivated by a short heat treatment of 15 minutes at 65°C.
This added convenience through complete heat inactivation has made SAP one of the most sold DNA modifying enzymes.
For added flexibility, especially when lyophilisation may be desired, rSAP is also available in a Glycerol FREE format. First launched in 1993, SAP’s unique features continue to make it a valuable tool in various applications.
Key Features
Gold standard heat labile, all-purpose alkaline phosphatase.
Completely inactivated after 5 min at 65°C or 1 min at 75°C.
Robust: Active in most restriction enzyme buffers, no need for supplemental zinc or other additives for activity.
Simple, hassle-free dephosphorylation of DNA, RNA, dNTPs and proteins for subsequent use in cloning or end-labelling of probes.
No vector purification necessary.
Easy inactivation of unincorporated dNTPs in PCR products prior to DNA sequencing or SNP analysis.
Ideal for MALDI-TOF, cloning, HLA typing, genotyping and sequencing.
Preparing substrate in protein kinase studies.
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ArcticZymes’ Shrimp Alkaline Phosphatase (SAP), including its recombinant version (rSAP), is the gold standard and the first heat-labile, all-purpose alkaline phosphatase on the market.
Methyltetrazine-PEG7-DBCO is a PEG linker with a terminal TCO reactive reagent and a DBCO group. DBCO is commonly used for copper-free Click Chemistry reactions. Methyltetrazine can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Methyltetrazine-PEG7-DBCO is a PEG linker with a terminal TCO reactive reagent and a DBCO group. DBCO is commonly used for copper-free Click Chemistry reactions. Methyltetrazine can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.