Introduction
Discover Huankai’s GC Agar Base, designed for the selective cultivation and isolation of Neisseria gonorrhoeae and Neisseria meningitidis, ensuring reliable and accurate microbial detection.
Discover Huankai’s GC Agar Base, designed for the selective cultivation and isolation of Neisseria gonorrhoeae and Neisseria meningitidis, ensuring reliable and accurate microbial detection.
Discover Huankai’s GC Agar Base, designed for the selective cultivation and isolation of Neisseria gonorrhoeae and Neisseria meningitidis, ensuring reliable and accurate microbial detection.
Sample type purification kit guide
The 16S V1-V2 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V1-V2 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The 16S V1-V2 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V1-V2 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
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| Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
| Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s 16S V1-V2 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
| Step | Component | Cat. 70100 (24 preps) | Cat. 70110, 70120, 70130, 70140 (96 preps) |
|---|---|---|---|
| Amplicon PCR (PCR 1) | MGX Master Mix | 330 µL | 1,320 µL |
| 16S V1-V2 Primer Mix | 70 µL | 280 µL | |
| Index PCR (PCR 2) | Indexing Master Mix | 660 µL | 2 x 1,320 µL |
| N7xx Index Primer | 50 µL | 50 µL | |
| S5xx Index Primer | 70 µL | 70 µL | |
| PCR Clean-Up | Resuspension Buffer | 2 x 1,250 µL | 2 x 5,000 µL |
| Nuclease-free water | 1,250 µL | 1 x 6,000 µL |
TXK4 Blood card centrifuge, ID card Centrifuge, Gel card centrifuge
Application:
TXK4 blood group serological tests multipurpose centrifuge widely used in the test of blood group serology, blood type, Microcolumn agglutination, immunodetection and etc.
Features:
1. Brushless motor to make sure free maintenance,no powder polution, micro computer control and digital display the time, speed and RCF to let operation easily.
2. Special centrifugation technical to different reagent according to the requirement.
3. Regular centrifugation program, customers can press the button to choose the required one.
4. Electrical lid lock, over speed and imbalance protection.
Technical Parameters:
| Max speed | 4000rpm | Max RCF | 2250xg |
| Max volume | 24 cards | Noise | ≤45dBA |
| Timer | 0～99min | Net weight | 23KG |
| Dimension(HxDxW) | 483×320×265mm | Power supply | AC 220 50HZ 2A |
| Speed accuracy | ±10rpm | Package | carton box |
Matched Rotors for TXK4:
| Order No. | Rotor Type | Max Speed (rpm) | Max Volume (ml) | Max RCF(g) |
| NO33183 | Swing Rotor | 3000 | 24 cards | 1080 |
| 4000 | 24 cards | 2120 | ||
| NO31605 | Fixed Rotor | 4000 | 30×7/5ml | 2250 |
| NO31601 | Fixed Rotor | 4000 | 12×10ml | 2150 |
TXK4 blood group serological tests multipurpose centrifuge widely used in the test of blood group serology, blood type, Microcolumn agglutination, immunodetection and etc.
K-YBGL
SKU: 700004358
100 assays per kit
| Content: | 100 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | β-Glucan |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 510 |
| Signal Response: | Increase |
| Linear Range: | 4 to 100 μg of glucose per assay |
| Limit of Detection: | 1 g/100 g |
| Total Assay Time: | ~ 100 min |
| Application examples: | Yeast preparations, mushroom preparations and other materials. |
| Method recognition: | Novel method |
The β-Glucan Assay Kit (Yeast and Mushroom) is suitable for the indirect measurement of 1,3:1,6-β-glucan in yeast and mushroom preparations containing starch, glycogen, sucrose and trehalose.
We are happy to announce a new and improved procedure!
Trehalase enzyme has now been added to the β-Glucan Assay Kit (Yeast and Mushroom), further increasing the accuracy of β-glucan measurement for certain samples. The addition of trehalase removes instances of overestimation of β-glucan content in samples containing trehalose. Please refer to the assay protocol and the FAQ here for more information.
This product replaces the Enzymatic Yeast β-Glucan Assay Kit (K-EBHLG) as the method of measurement for β-glucan measurement in yeast samples. See here for more information.
See our complete list of available polysaccharide assay kits.
Advantages
The β-Glucan Assay Kit (Yeast and Mushroom) is suitable for the indirect measurement of 1,3:1,6-β-glucan in yeast and mushroom preparations containing starch, glycogen, sucrose and trehalose.