Introduction
Discover Huankai’s Lysine Iron Agar, designed for the differentiation and identification of enteric bacteria, particularly Salmonella, through lysine decarboxylation and hydrogen sulfide production.
Discover Huankai’s Lysine Iron Agar, designed for the differentiation and identification of enteric bacteria, particularly Salmonella, through lysine decarboxylation and hydrogen sulfide prod……
Discover Huankai’s Lysine Iron Agar, designed for the differentiation and identification of enteric bacteria, particularly Salmonella, through lysine decarboxylation and hydrogen sulfide production.
| Clone | IHC583 |
| Source | Mouse Monoclonal |
| Positive Control | Breast Carcinoma, Urothelial Carcinoma |
| Dilution Range | 1:200 |
GATA3 is a transcription factor important in cell proliferation, development, and differentiation. GATA3 is mostly observed in breast and urothelial carcinomas, and rarely present in other cancers such as endometrial endometrioid adenocarcinoma. Among the breast carcinomas, GATA3 has a lower expression in luminal B subtype breast carcinoma. Studies have found GATA3 expression to be associated with ER (estrogen receptor), PR (progesterone receptor), and Her2 in breast cancer cases. Urothelial carcinomas stain positively for GATA3 in invasive or high grade tumors, therefore Anti-GATA3 is useful for carcinoma diagnosis when breast and bladder are plausible.
These kits provide a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from mammalian blood samples. These kits are supplied with an RBC (red blood cell) Lysis Buffer for selective removal of red blood cells and fractionation of leukocytes by centrifugation. Isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. These kits are able to isolate total leukocyte RNA, including both large mRNA and all small RNA species containing microRNA (miRNA) and small silencing RNA (siRNA). The purified RNA is of the highest quality and can be used in a number of downstream applications.
Leukocyte RNA Purification Kit (Spin Column)
This kit provides a rapid method for the isolation and purification of total leukocyte RNA from mammalian blood samples in 40 minutes. Allowable blood input ranges from 10 μL to 2 mL or 3 x 106 Leukocytes
Leukocyte RNA Purification Plus Kit (Plus)
Norgen’s Leukocyte RNA Purification Plus Kit provides a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from up to 3 mL of mammalian blood samples. Complete 10 purifications in as little as 40 minutes.
Leukocyte RNA Purification 96-Well Kit (High Throughput)
Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Norgen’s kit allows for the isolation of total leukocyte RNA, including all small RNA species. The purified RNA is of the highest quality and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, northern blotting, RNase protection and primer extension, and expression array assays. Allowable blood input ranges from 10 μL to 1 mL or 1.5 x 106 Leukocytes.
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| Kit Specifications | |
| Maximum Column Binding Capacity | 10 µg |
| Maximum Column Loading Volume | 650 μL |
| Size of RNA Purified | All sizes, including small RNA (<200 nt) |
| Maximum Blood Input Volume | 3 mL |
| Time to Complete 10 Purifications | 40 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RBC Lysis Buffer should be stored at 4°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.
| Component | Cat. 21200 (50 preps) | Cat. 21250 (50 preps) | Cat. 37800 (2 x 96 preps) |
|---|---|---|---|
| RBC Lysis Buffer | 2 x 100 mL | 2 x 1 L | 2 x 90 mL |
| Buffer RL | 30 mL | 30 mL | – |
| Binding Solution | – | – | 2 x 40 mL |
| Wash Solution A | 38 mL | 38 mL | – |
| Wash Solution | – | – | 2 x 30 mL |
| Elution Solution A | 6 mL | 6 mL | – |
| Elution Solution | – | – | 2 x 20 mL |
| Enzyme Incubation Buffer | – | 6 mL | 1 |
| DNase I | – | 1 Vial | – |
| Mini Spin Columns | 50 | – | – |
| Lysate Homogenization Column | – | 50 | – |
| Single Cell RNA Column | – | 50 | – |
| RBC Lysis 96-Well Plate | – | – | 2 |
| 96-Well Filter Plate | – | – | 2 |
| Adhesive Tape | – | – | 4 |
| Collection Tubes | 50 | 100 | – |
| 96-Well Collection Plate | – | – | 2 |
| Elution Tubes (1.7 mL) | 50 | 50 | – |
| 96-Well Elution Plate | – | – | 2 |
| Product Insert | 1 | 1 | 1 |
It is suitable for the extraction and purification of viral nucleic acid and free nucleic acid. It can bind the nucleic acids in solution through hydrophobic, hydrogen bonding and electrostatic interaction under high salt conditions, without binding with other impurities (such as proteins), and quickly separate nucleic acids from biological samples. The operation is safe and simple, which is very conducive to the automatic and high-throughput extraction of short fragments or low abundance nucleic acids.
Note: Price not include shipment & duty, contact us to get full quote.
Magnetic beads are specially designed for nucleic acid extraction and purification, with good suspension performance. The surface is modified with a large number of carboxyl groups, which can bind the nucleic acid in the sample through hydrophobic, hydrogen bonding and electrostatic interaction under high salt and low pH conditions, without binding with other impurities (such as proteins), and quickly separate nucleic acid from biological samples. The operation is safe and simple. It is beneficial to the automation and high throughput extraction of nucleic acid.