Intended Use For the enumeration of enterococci in water and other liquids by the membrane filtration technique. Principle and Interpretation The growth of the entire accompanying Gram-neg……
Detail
Introduction
Intended Use
For the enumeration of enterococci in water and other liquids by the membrane filtration technique.
Principle and Interpretation
The growth of the entire accompanying Gram-negative microbial flora is inhibited by sodium azide. Enterococci reduce 2,3,5-Triphenyl tetrazolium chloride (TTC) to give a red formazan inside the bacterial cell, their colonies are thus red. Nitrogen, minerals, and amino acids are provided by the tryptose whilst yeast extract supplies vitamins. Glucose acts as the carbon source, dipotassium phosphate buffers the medium, and agar-agar is the solidifying agent.
Formulation
Ingredients
/liter
Tryptose
20.0g
Yeast extract
5.0g
Glucose
2.0g
Disodium hydrogen phosphate
4.0g
Sodium azide
0.4g
2,3,5-Triphenyl Tetrazolium chloride
0.1g
Agar
10.0g
pH 7.2±0.1 at 25°C
Preparation
Dissolve 41.5 g in 1 L of purified water. Heat in boiling water and agitate frequently until completely dissolved. Sterilize by further heat for 20 minutes in the boiling water bath.
Quality Control
Cultural characteristics observed after incubation at 34-38°C for 40-48hours
Quality control strains
Growth
Colony color
Enterococcus faecalis ATCC29212
Good growth,PR≥0.5
deep red coloured colonies
Escherichia coli ATCC25922
Total inhibition
–
Sorage and Shelf Life
Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Other Products
LowRanger 100 bp DNA Ladder
Product Info
Document
Product Info
Overview
Ready-to-use
Quantitative
Highly Stable
Precise
Eleven discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments
Higher intensity reference band at 500 bp
The Norgen LowRanger 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our LowRanger contains eleven discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments with a higher intensity reference band at 500 bp. This Ladder is ideal for fast running times and accurate visual determination.
Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
LowRanger 100bp DNA Ladder (Cat# 11500) – 100 loads
Ladder Properties: • Eleven discrete bands, ranging from 100 bp to 2000 bp • Higher intensity band at 500 bp for easy reference.
Fragment
Size (bp)
Mass (ng)
1
2000
110
2
1500
83
3
1000
55
4
800
44
5
700
39
6
600
33
7
500
55
8
400
22
9
300
17
10
200
22
11
100
22
Recommended Use: Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage: Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Eleven discrete fragments ranging from 50 bp to 1000 bp
Higher intensity reference band at 500 bp
The Norgen PCR Ranger 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our PCR Ranger DNA Ladder contains eleven discrete fragments ranging from 50 bp to 1000 bp with a higher intensity reference band at 500 bp. This Ladder is ideal for assessment of a range of PCR product sizes.
Contents 1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
PCR Ranger 100 bp DNA Ladder (Cat# 11300) – 100 loads
Ladder Properties: • Eleven discrete bands, ranging from 50 bp to 1,000 bp • Higher intensity band at 500 bp for easy reference
Fragment
Size (bp)
Mass (ng)
1
1000
91
2
900
66
3
800
61
4
700
51
5
600
43
6
500
70
7
400
28
8
300
23
9
200
30
10
100
22
11
50
15
Recommended Use: Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage: Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
These kits provides a rapid method for the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva collected on Norgen’s Saliva RNA Collection and Preservation Devices Dx (Cat. 53800) or preserved swabs collected in Norgen’s Total Nucleic Acid Preservative Tubes Dx (Cat. Dx69200). Purification is based on using Norgen’s proprietary resin separation matrix. RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The chemistry employed in the kit allows the purification of total RNA, including viral and bacterial RNA, irrespective of size or GC content. The purified RNA is ideal for in vitro diagnostic use for medical purposes.
Saliva/Swab RNA Purification Kit Dx (Spin Column)
This kit is optimized to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Norgen’s Saliva / Swab RNA Purification Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.
To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used.
Norgen’s Saliva/Swab RNA Purification Kit Dx is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques.
Norgen’s Saliva/Swab RNA Purification Kit Dx does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.
This kit is optimized to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Norgen’s Saliva / Swab RNA Purification 96-Well Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings. To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used. Norgen’s Saliva / Swab RNA Purification 96-Well Kit Dx is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques. Norgen’s Saliva / Swab RNA Purification 96-Well Kit Dx does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.
High throughput 96-well plate format for rapid purifications
Very consistent well-to-well RNA isolation
Bind & elute all RNA irrespective of size or GC content, without bias
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. All solutions and plastics can be used until the expiration date specified on their labels.
