029050 VP Reagent Veoges-Proskauer

Description

Our SNPsig® kits use our own proprietary genotyping method to enable the identification of SARS-CoV-2 variants of concern. These products can be used on any real-time PCR machine using familiar protocols, whilst resulting in exceptional genotyping data.

Positive control templates for wild-type and variants are supplied in every kit to make data interpretation simple.

Our SNPsig® technology provides an alternative to sequencing as well as S gene target failure (SGTF) that enables scientists to analyse and monitor these specific genomic mutations.  Our kits can provide a pivotal role in screening for SARS-CoV-2 variants for the purpose of genomic surveillance and studies.

For the detection of the SARS-CoV-2 variants with the VUI-21APR-01 and VUI-21APR-03 (India)
Rapid detection of specific detection profiles
High priming efficiency
Sensitive to < 100 copies of target Positive copy number standard curve for quantification Accurate controls to confirm findings 96 reactions, includes master mix

Overview

• Reduce endotoxin levels to 0.1 EU/µg DNA or less
• Available in 3 formats: Mini, Midi, and Maxi to suit your desired input volume
• Remove endotoxins from up to 1 mg of DNA with the Maxi format kit
• Fast and easy processing using a rapid spin-column format

These kits are designed for the rapid spin column removal of endotoxins from previously purified DNA. Norgen’s spin columns bind DNA while endotoxins, salts and other contaminants are washed away. These kits reduce endotoxins to 0.1 EU/μg DNA or less providing plasmid DNA that is immediately ready for transfections or other endotoxin-sensitive applications. Typical recovery of DNA is >90% of the starting sample.

Endotoxin Removal Kit (Mini)

This kit is designed for the rapid spin column removal of endotoxins from up to 25 µg of previously purified DNA. The convenient spin column procedure can be completed in approximately 20 minutes.

Endotoxin Removal Kit (Midi)

This kit is designed for the rapid spin column removal of endotoxins from up to 200 μg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.

Endotoxin Removal Kit (Maxi)

This kit is designed for the rapid spin column removal of endotoxins from up to 1 mg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.

About Endotoxins

Endotoxins (also called lipopolysaccharides), are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin sensitive cell lines. Therefore, the removal of endotoxins from plasmid preparations is often necessary prior to the use of the DNA in such downstream applications.

Details

Supporting Data

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Kit Specifications
Maximum DNA Input	25 μg
Final Endotoxin Level	≤ 0.1 EU/µg DNA
Size of DNA Purified	Up to 13,000 bp
Maximum DNA Volume Input	100 μL
Average Recovery	> 90%
Time to Complete 10 Purifications	20 minutes

 
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 22700 (25 preps)	Cat. 52200 (10 preps)	Cat. 21900 (4 preps)
Buffer SK	15 mL	60 mL	60 mL
Wash Solution H	18 mL	18 mL	18 mL
Elution Buffer I	6 mL	12 mL	12 mL
Endotoxin Removal Solution	1.5 mL	1.5 mL	1.5 mL
Precipitation Solution	–	1.5 mL	1.5 mL
Spin Columns (assembled with Collection Tubes)	–	10	4
Spin Columns	25	–	–
Collection Tubes	25	–	–
Elution Tubes (1.7 mL)	25	–	–
Elution Tubes (15 mL)	–	10	–
Elution Tubes (50 mL)	–	–	4
Product Insert	1	1	1