Usages: For monitoring and detection of planktonic bacteria and settlement bacteria in pharmaceutical industry & clean room.
Advantage:
1.This series of products was filling at A level of environment, and final sterilization by Gamma ray irradiation, triple packaging insure sterility and long shelf life. 2.Each dish was marking label product name, batch number, expiration date .Information is available of traceability. 3.Inner additional desiccant, reducing formation of condensation water, while inner additional sterile paper and plastic bags for easier transfer and cultivation. 4.Triple packing dense bags to avoid the penetration of hydrogen peroxide; clean gas was filled as a buffer to reduce broken bags and dish in transit. 5.The 90mm medium plate loading capacity of 30mL, to meet requirement of the environmental sampling for long exposure of up to 4h and 5-7 days of culture. 6. This series of products is available to store at (2-25 ℃), shelf life of up to 6 months.
Storage: Store in a cool (2-25 ℃), dry place, away from bright light. Storage period of 6 months.
Specifications: 90mm*10 plates / bag
Other Products
HAM009 Yeast Extract Powder
Product Info
Document
Product Info
Introduction
Usages: Yeast extract is made from yeast, used in microbiological culture media as a source of nitrogen and B vitamins.
Technical specification: Total nitrogen(TN)—————————≥10.0% Amino nitrogen(AN) ————————≥4.0% Moisture—————————————≤5.0% Ash———————————————≤10.0% Clˉ——————————————–＜2.0%
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light.
Hipure Stool RNA Kit is specially designed for stool RNA extraction. This kit is suitable for extracting high-purity microbial or host cell RNA from ≤0.1g stool samples. The kit adopts silica gel column purification technology and original solution system, which can effectively remove humic acid and other inhibitory factors in stool samples. The purified RNA can be directly used in RT-PCR, Northern hybridization and other experiments.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from 100-150mg stool sample
Applications
RT-PCR, Northern hybridization and other experiments
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Stool
Sample amount
100-150 mg
Elution volume
≥30μl
Time per run
≤50 minutes
Liquid carrying volume per column
100µg
Binding yield of column
800µl
Principle
The HiPure silica gel column uses a high binding ability glass fiber filter membrane as the substrate. Under the condition of high concentration of ionizing agent (such as Guanidinium chloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bonding and electrostatic and other physical factors, while protein or other impurities are not adsorbed and removed. The filter membrane that has adsorbed nucleic acids is washed to remove proteins and salts. Finally, low salt buffer solution (such as Buffer TE) or water can be used to wash out the nucleic acids adsorbed on the filter membrane. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
The stool samples are homogenized in the lysis solution, further lysed in a high-temperature water bath, and RNA is released into the lysis solution. Chloroform extraction removes genomic DNA and impurities, transfer the supernatant to an alcohol free binding solution, purify RNA through a column, and finally elute RNA with RNase Free Water. The purified RNA can be directly used for experiments such as PCR, Southern hybridization, and enzyme digestion.
Advantages
High purity – unique adsorbent for more efficient removal of inhibitors
High concentration – maximum extraction of total RNA from stool samples
Sensitive – RNA can be purified at the level of PG
Kit Contents
Contents
R418502
R418503
Purification Times
50 Preps
250 Preps
HiPure RNA Mini Columns
50
250
2ml Collection Tubes
50
250
Glass Beads (0.1~0.6mm)
30 g
150 g
Buffer SPL
30 ml
140 ml
Buffer PHC
30 ml
140 ml
Buffer GRP
60 ml
250 ml
Buffer RW1
50 ml
250 ml
Buffer RW2 *
20 ml
2 x 50 ml
RNase Free Water
15 ml
30 ml
Storage and Stability
The kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions. At low temperatures, Buffer SPL may form precipitates, dissolve it by 55°C water bath. After receiving the product, Buffer PHC should be stored at 2-8°C.
Document
Hipure Stool RNA Kit is specially designed for stool RNA extraction. This kit is suitable for extracting high-purity microbial or host cell RNA from ≤0.1g stool samples. The kit adopts silica gel column purification technology and original solution system, which can effectively remove humic acid and other inhibitory factors in stool samples. The purified RNA can be directly used in RT-PCR, Northern hybridization and other experiments.
HiPure DNA Clean Up Kit usesproprietary chemistry and HiPure technology to recover DNA Fragments between20bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing,restriction digestion, or various labeling reactions. In addition, this kit canbe also used to recover DNA directly from enzymatic reactions such as PCR andenzyme digestion reactions.
Details
Specifications
Features
Specifications
Main Functions
Recover DNA fragments between 20bp-20kbp from crude DNA and enzymatic reaction solution
Applications
PCR, sequencing, labeling reactions, ligations and restriction digestion, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Crude DNA, enzymatic reaction solution
Sample amount
<150μl
Recovery
80%
Elution volume
≥15μl
Time per run
≤10 minutes
Liquid carrying volume per column
800µl
Binding yield of column
20µg
Principle
The HiPure system uses a simple bind-wash-elute procedure. Gelslices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
High recovery efficiency – up to 80% DNA recovery
Fast – isolation can be completed in 10 minutes by column gel method
Low elution volume – elution volume can be as low as 10μl
Efficient – efficient removal of various inhibitory factors
Kit Contents
Contents
D214102
D214103
Purification Times
100 Preps
250 Preps
Buffer GWP
30 ml
60 ml
Buffer DW2
20 ml
50 ml
Elution Buffer
30 ml
60 ml
HiPure DNA Mini Columns I
50
250
2 ml Collection Tubes
50
250
Storage and Stability
HiPure DNA Clean Up Kit components are guaranteed for at least one year when stored at room temperature. If any precipitates form in the buffers, warm at 37℃ to dissolve.
Document
HiPure DNA Clean Up Kit usesproprietary chemistry and HiPure technology to recover DNA Fragments between20bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing,restriction digestion, or various labeling reactions. In addition, this kit canbe also used to recover DNA directly from enzymatic reactions such as PCR andenzyme digestion reactions.
