Usages: For selective cultivation of bacteria especially Vibrio.
Principle: Peptone provide carbon and nitrogen sources; sodium chloride to maintain osmotic equilibrium; higher pH can suppress the growth of coliform and other bacteria, it is conducive to the growth of Vibrio cholerae.
How to use： 1.Suspend 18g in 1L of distilled water, stirring heated to boiling to completely dissolve, autoclave at 121℃ for 15 minutes. 2.Diluted and treated samples.
Quality control：
Item
The name and number of strain
Growth
Colony Color
1
Vibrio cholerae VbO
Good
Turbid broth
2
Escherichia coli ATCC25922
Poor
—
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 250g/bottle
Other Products
resDNASEQ E.coli Residual DNA Quantitation kit
Product Info
Document
Product Info
Description
Features of the sDNASEQ E.coli Residual DNA Quantitation kit include:
Simpler and Rapid
Only three steps will be need for Sample Preparation and All components of the Sample Preparation Kit can be stored at room temperature.
Only one Reagent for qPCR;
Only 1.5 hours will be needed for the whole test.
Accurate
Perfect amplification curve, good amplification efficiency and good precision.
Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology.
Limit of Detection (LoD): 1 fg/μL; Limit of Quantification (LoQ):5 fg/μL.
The recovery rate of different concentration samples in the linear range is between 70% and 130%
Kit Performance
Fig 1. Only three steps will be need for Sample Preparation and only 20 minitutes will be taken for Sample Preparation.
Fig 2. Seven concentration samples of 5fg/μL, 10fg/μL, 20fg/μL, 30fg/μL, 300fg/μL, 3pg/μL, 30pg/μL, 300pg/μL were detected. CV of each concentration was < 30%, Regression coefficient associated with standard solutions was 0.99975, and amplification efficiency was 100.068%.
Fig 3. Four concentration samples of 5fg/μL, 10fg/μL, 20fg/μL, and 30fg/μL were detected, and 10 multiple holes were detected for each concentration. The detection values of 5fg/μL and above were CV ＜30%.
Fig 4. DNA recovery can be determined by including samples spiked with known DNA amounts which are prepared from the corresponding DNA standards. Typically, the range for this value varies from 70% to 130%.
Fig 5. Only one Reagent for qPCR MIX.
Document
Note: Price not include shipment & duty, contact us to get full quote. The resDNASEQ E.coli Residual DNA Quantitation kit is designed for the quantification of residual DNA from E. coli, in cell lines which are used for production of biopharmaceutical products. The resDNASEQ E.coli Residual DNA Quantitation kit use TaqManTM quantitative PCR to perform rapid, specifc quantitation of femtogram levels of residual host-cell or plasmid DNA. The kit was developed to meet the sensitivity requirements defined by WHO (10 ng E. coli DNA per therapeutic dose).
[RI1001] RNAok™ RNase Inhibitor, 20 U/μl, 2000 U x 5
Product Info
Document
Product Info
Description
The RNAok™ RNase Inhibitor is a recombinant mammalian RNase inhibitor which possesses very high affinity for eukaryotic pancreatic-type ribonuclease. The RNAok™ RNase Inhibitor forms a 1:1 complex with pancreatic RNase A by noncovalent binding, presenting a noncompetitive inhibitory activity on these pancreatic enzymes. RNAok™ RNase Inhibitor is active against RNase A, RNase B, RNase C but not RNAse H, RNase I, RNase T1, RNase T2, and S1 nuclease. RNAok™ RNase Inhibitor is compatible with RT-PCR enzymes such as AMV, M-MLV and ExcelRT™ Reverse Transcriptase or Taq DNA polymerase.
Application
cDNA Synthesis
in vitro translation
in vitro transcription
One-step RT-PCR
Separation and identification of specific ribonuclease activities
Storage Buffer
40 mM HEPES-KOH (pH 7.5), 100 mM KCl, 8 mM DTT, 0.1 mM EDTA, stabilizer and 50% (v/v) glycerol
Storage
-20°C for 24 months
Document
The RNAok™ RNase Inhibitor is a recombinant mammalian RNase inhibitor which possesses very high affinity for eukaryotic pancreatic-type ribonuclease. The RNAok™ RNase Inhibitor forms a 1:1 complex with pancreatic RNase A by noncovalent binding, presenting a noncompetitive inhibitory activity on these pancreatic enzymes. RNAok™ RNase Inhibitor is active against RNase A, RNase B, RNase C but not RNAse H, RNase I, RNase T1, RNase T2, and S1 nuclease. RNAok™ RNase Inhibitor is compatible with RT-PCR enzymes such as AMV, M-MLV and ExcelRT™ Reverse Transcriptase or Taq DNA polymerase.
An adapter for Cerillo’s Stratus plate reader. This adapter allows the Stratus plate reader to fit perfectly on the deck of the Opentrons Flex or OT-2 to allow for pipetting directly into the reader.
Document
An adapter for Cerillo’s Stratus plate reader. This adapter allows the Stratus plate reader to fit perfectly on the deck of the Opentrons Flex or OT-2 to allow for pipetting directly into the reader.
